Vav2 (BC053060) Mouse Tagged ORF Clone

CAT#: MR210853

Vav2 (Myc-DDK-tagged) - Mouse vav 2 oncogene (cDNA clone MGC:62363 IMAGE:5704852)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vav2
• Synonyms: 2810040F13Rik|AI847175|Vav-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210853 representing BC053060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGGCGGCAATGCGGCCGCTGGCTCATCGACTGCAAGGTCCTGCCGCCCAACCACCGCGTCG
  TGTGGCCCTCGGCGGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGCGTCCTTCTGTGCCAGCTGCT
  GCACAACCTCTCCCCCGGTTCCATCGACCTTAAGGACATCAACTTCCGGCCGCAGATGTCCCAGGTCATC
  TCTGCAGTGTCCCGTCTGTCCCTGCACAGCATCGCACAGAGCAAAGGGATCAGGCCATTTCCATCAGAGG
  AGACAGCTGAAAATGATGACGACGTCTACCGGAGCCTGGAAGAGCTGGCTGACGAGCATGACCTGGGTGA
  GGACATCTACGACTGTGTCCCGTGTGAAGATGAAGGAGATGACATTTATGAGGACATCATCAAGGTGGAG
  GTGCAGCAGCCCATGATCAGATACATGCAGAAAATGGGCATGACTGAGGACGACAAGAGAAGCTGCTGCT
  TGTTAGAGATTCAGGAGACCGAGGCCAAGTACTACCGCACCCTGGAGGACATTGAGAAGAACTACATGGG
  TCCCTTGCGGCTGGTGCTGAGCCCGGCGGATATGGCTGCTGTCTTCATCAACCTGGAGGACCTCATCAAG
  GTGCATCACAGCTTTCTGCGAGCCATCGATGTGTCCATGATGGCTGGTGGCAGTACCCTGGCTAAGGTCT
  TTCTGGAGTTTAAGGAAAGGCTCCTGATCTATGGAGAGTACTGTAGCCACATGGAACACGCTCAGAGTAC
  ACTGAACCAGCTCCTCGCCAGCCGAGAGGACTTCAGGCAGAAAGTGGAGGAGTGCACACTCAGGGTTCAG
  GATGGCAAGTTCAAGCTGCAAGACCTGCTGGTGGTGCCCATGCAACGGGTGCTGAAGTACCACCTGCTGC
  TCAAGGAGCTCCTGAGCCATTCTGCAGACCGACCAGAAAGACAACAGCTCAAAGAAGCCCTGGAAGCCAT
  GCAGGACTTGGCCATGTACATCAATGAAGTGAAGCGGGACAAGGAGACCTTGAAGAAGATTAGCGAGTTC
  CAGTGCTCCATAGAAAACCTGCAAGTGAAGCTGGAGGAATTTGGGAGGCCAAAGATTGACGGGGAGCTTA
  AAGTCCGGTCCATAGTCAACCACACCAAGCAAGACAGGTACCTGTTCCTATTTGACAAGGTGGTCATCGT
  GTGCAAGAGGAAGGGCTACAGCTATGAGCTGAAGGAGGTCATTGAGCTGCTCTTCCACAAGATGACCGAT
  GACCCGATGCACAACAAGGACATCAAGAAGTCTCATGGGAAGATGTGGTCCTATGGCTTCTACCTGATTC
  ACCTCCAAGGAAAGCAAGGCTTTCAGTTCTTCTGCAAGACGGAAGACATGAAGCGGAAGTGGATGGAGCA
  GTTCGAGATGGCCATGTCAAACATCAAGCCAGATAAGGCCAATGCCAACCATCATAGCTTCCAGATGTAC
  ACATTCGACAAGACTACCAACTGCAAAGCCTGCAAGATGTTTCTCAGGGGTACCTTCTACCAGGGATACC
  TGTGTACCAGATGTGGCGTCGGGGCACACAAGGAATGCCTGGAGGTGATCCCCCCCTGCAAGATGAGTTC
  ACCTGCAGATGTGGATGCTCCTGGAGCAGGACCAGGTCCCAAGATGGTTGCTGTGCAGAATTACCATGGT
  AACCCAGCCCCTCCTGGGAAGCCTGTGTTGACCTTCCAGACAGGAGACGTGATAGAACTGCTCCGGGGTG
  ACCCCGATTCTCCTTGGTGGGAAGGGCGGCTGGTTCAAACCAGGAAGTCAGGGTATTTTCCCAGCTCATC
  TGTGAAGCCCTGCCCAGTGGATGGAAGGCCGCCTACTGGCCGCCCACCATCCCGGGAGATCGATTACACT
  GCCTACCCGTGGTTTGCTGGCAACATGGAACGGCAGCAGACGGACAATCTGCTCAAGTCTCATGCCAGTG
  GGACCTACCTCATCAGAGAGCGTCCAGCAGAGGCAGAACGCTTCGCCATCAGCATCAAGTTCAATGATGA
  GGTGAAACATATCAAGGTGGTGGAGAAAGATAGCTGGATCCACATCACGGAAGCTAAGAAGTTTGAGAGC
  CTCCTGAGCATTCTTTCAGCAATATTTGGGAGCCCTGCGCCTGAGGGAGAGGTTTTTGAGGCACTGGCCA
  TCCTAGCTGGGCTTGGCTGGAGAGCTGGTGGAGTACTACCAGTGTCACTCACTCAAGGAAAGCTTCAAGC
  AGTTGGACACTACACTCAAGTTCCCCTACAAGTCTCGGGAGCGAACCACCAGCAGGGCCTCAAGCCGGTC
  TCCAGCTTCCTGCGCTTCCTACAACTTTTCTTTTCTCAGTCCTCAGGGCCTCAGCTTTGCCCCCCAGGCC
  CCTTCTGCTCCCTTCTGGTCAGTGTTCACACCGCGGGTCATCGGCACAGCCGTGGCCAGGTACAACTTTG
  CTGCTCGAGACATGCGGGAGCTGTCACTGCGAGAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210853 representing BC053060
  Red=Cloning site Green=Tags(s)
  
  MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQVI
  SAVSRLSLHSIAQSKGIRPFPSEETAENDDDVYRSLEELADEHDLGEDIYDCVPCEDEGDDIYEDIIKVE
  VQQPMIRYMQKMGMTEDDKRSCCLLEIQETEAKYYRTLEDIEKNYMGPLRLVLSPADMAAVFINLEDLIK
  VHHSFLRAIDVSMMAGGSTLAKVFLEFKERLLIYGEYCSHMEHAQSTLNQLLASREDFRQKVEECTLRVQ
  DGKFKLQDLLVVPMQRVLKYHLLLKELLSHSADRPERQQLKEALEAMQDLAMYINEVKRDKETLKKISEF
  QCSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEVIELLFHKMTD
  DPMHNKDIKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMY
  TFDKTTNCKACKMFLRGTFYQGYLCTRCGVGAHKECLEVIPPCKMSSPADVDAPGAGPGPKMVAVQNYHG
  NPAPPGKPVLTFQTGDVIELLRGDPDSPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPTGRPPSREIDYT
  AYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDSWIHITEAKKFES
  LLSILSAIFGSPAPEGEVFEALAILAGLGWRAGGVLPVSLTQGKLQAVGHYTQVPLQVSGANHQQGLKPV
  SSFLRFLQLFFSQSSGPQLCPPGPFCSLLVSVHTAGHRHSRGQVQLCCSRHAGAVTARR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053060
• ORF Size: 2487 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053060, AAH53060
• RefSeq Size: 5113 bp
• RefSeq ORF: 2489 bp
• Locus ID: 22325
• MW: 187.4 kDa
• Gene Summary: This gene encodes a member of the Vav family of Rho guanine nucleotide exchange factors. Vav family proteins are involved in the development and activation of lymphocytes, and the encoded protein may also be involved in angiogenesis. Disruption of this gene in mice is associated with heart, artery, and kidney defects, as well as tachycardia and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]