L3mbtl3 (BC082309) Mouse Tagged ORF Clone

CAT#: MR210974

L3mbtl3 (Myc-DDK-tagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3mbtl3
• Synonyms: AI481284|MBT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210974 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAATCTGCATCTAGCACAAGTGGTCAAGAGTTTGATGTGTTTAGTGTTATGGACTGGAAGGATG
  GCGTTGGTACCCTACCAGGGAGTGATTTAAAGTTTCGAGTAAATGAGTTTGGAGCTTTGGAAGTTATCAC
  AGACGAAAGCGAGATGGAAAGTGTTAAGAAAGCTACAGCCACTACCACATGGATGGTCCCAACTGCTCAA
  GACGTCTTTTCAGAGAAGACTGGGGTACCCTTCAGGCTGAAGGAGCAATCAAAAGCAGACGGGCTTCAGT
  TTTGTGAGAACTGTTGTCAGTATGGCAACGGAGATGAATGTCTTTCGGGAGGAAAGTATTGCAGCCAGAA
  TTGTGCTCGGCATGCCAAAGACAAAGATCAAAAGGATGAACGGGATGGAGGCGAAGACAACGATGAGGAA
  GATCCTAAGTGTAGTCGAAAGAAAAAGCCAAAATTGTCTCTGAAGGCGGACTCCAAGGACGATGGAGAAG
  AGAGAGATGATGAAATGGAGAACAAACAAGATGGAAGGATCCTGAGGGGTTCTCAGAGAGCCAGAAGGAA
  AAGACGCGGGGATTCTGCCGTCTTAAAGCAAGGCTTGCCTCCTAAAGGCAAGAAGACCTGGTGTTGGGCT
  TCCTACCTGGAGGAGGAGAAAGCAGTGGCTGTGCCTACGAAGCTGTTCAAAGAGCACCAATCCTTCCCAT
  ATAACAAAAATGGATTCAAAGTGGGCATGAAGCTAGAAGGCGTGGACCCTGACCACCAGGCAATGTACTG
  CGTCCTTACTGTGGCTGAGGTTTGTGGATATAGGATAAAACTTCACTTTGACGGTTATTCTGATTGCTAT
  GACTTCTGGGTAAATGCAGATGCTCTGGACATTCACCCAGTAGGCTGGTGTGAGAAGACTGGCCATAAGC
  TCCGCCCTCCAAAAGGGTATAAAGAAGAAGAATTTAATTGGCAGTCCTACCTTAAGACTTGCAAAGCCCA
  GGCTGCTCCCAAGTCATTATTTGAAAATCAGAACATAACAGTGATCCCGTCAGGCTTTCGAGTTGGGATG
  AAGCTCGAGGCTGCAGACAAAAAGAGCCCTTCTGTCATCTGTGTTGCTACGGTAACAGATATGGTGGACA
  ATCGTTTCCTGGTACATTTTGACAACTGGGATGAGAGCTATGACTATTGGTGTGAATCAAACAGTCCACA
  CATTCATCCAGTCGGCTGGTGTAAAGAGCACAGAAGAACCCTGATTACTCCACCAGGCTATTCTCATGTG
  AAACACTTTTCCTGGGATAAATATTTAGAAGAAACCAATTCTTTACCTGCTCCTGCAAGAGCTTTCAAAG
  TGAAACCTCCACATGGATTCCAGAAGAAAATGAAGCTGGAAGCTGTGGACAAAAGAAACCCGCTGTTCAT
  CAGAGTAGCAACTGTAGCCGACACGGATGACCACCGGATAAAGGTTCACTTTGATGGCTGGAGCAGTTGC
  TATGACTACTGGATTGATGCAGACTCTCCAGATATTCACCCCGTGGGCTGGTGTTCCAAGACCGGGCACC
  CTCTTCAGGCTCCGCTAAGCCCTGCAGAATTAATGGAACCATCAGAAACCGGAGGATGTCCAACTCTAGG
  CTGCAGGGGTGTTGGTCATTTTAAAAAATCGAGATACCTGGGCACTCAGAGTGGTGCCAACTGTCCCTAT
  TCAGAAATCAACCTGAGTAAGGAACGCATCTTCCCAGACCGCCTAAGTGGTGACACCTCTCCACCTACTA
  CTCCCTCGTTCCCAAGGAGTAAAAGGATGGACACCAGAGAAAGTTCCTCGTCTCCTGAAACCAGGGAAAA
  GCATGCCAATAACTTCAAAGAAGATTCTGAAAAGAAAAAAGAAAATGAAGTAAAGACATCAGCCGAAGCC
  AAAGTTGTTCGGGAAGAGCCTACCCCTAGTGTACAGCAGAGTCAGCCACCTCAGCAGGTGCAGCAAGTGC
  AGCATGCTCAACCTCCTCAGCAGGCACAGAAGGCTCCGCAGGCCCAGCAGGCCCAGCAAGCCCAGCAGGC
  CCAGCAGGCTCCACAGGCCCCACAGACTCCACAGCCCCAGCAGGCGCCACAGGTCCAGCAGGCCCAGCAG
  GCTCCACAGGCTCAGCAGGCCCAGCAGCCCCAGCAGGCCCAGCAGCCTCAGCAGGCCCCACCCGTCCAAC
  AGCCTCAGCAGGTTCAACAGGCTCAGCCCACCCAGCAGCAAGCCCAGACCCAGCAGCAGGCCCAGCGACG
  CTCAGCAGTATTTCTCTCCTTTAAGCCTCCCATTCCGTGTCTGCCCTTGCGCTGGGAGCAGCAGAGCAAG
  CTTCTTCCGACTGTCGCTGGTATCCCAGCTAGTAGAGTTTCCAAATGGAGCACAGACGAGGTGTCAGAAT
  TCATTCAGAGCTTGCCTGGGTGTGAAGAACATGGAAAGGTGTTTAAGGATGAACAAATTGATGGAGAAGC
  ATTTTTACTCATGACCCAAACAGACATTGTTAAAATCATGAGCATTAAGCTGGGCCCAGCTCTCAAAATT
  TTCAACTCTATTCTGATGTTCAAAGCTGCGGAGAAGAATTCCCACAATGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210974 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDESEMESVKKATATTTWMVPTAQ
  DVFSEKTGVPFRLKEQSKADGLQFCENCCQYGNGDECLSGGKYCSQNCARHAKDKDQKDERDGGEDNDEE
  DPKCSRKKKPKLSLKADSKDDGEERDDEMENKQDGRILRGSQRARRKRRGDSAVLKQGLPPKGKKTWCWA
  SYLEEEKAVAVPTKLFKEHQSFPYNKNGFKVGMKLEGVDPDHQAMYCVLTVAEVCGYRIKLHFDGYSDCY
  DFWVNADALDIHPVGWCEKTGHKLRPPKGYKEEEFNWQSYLKTCKAQAAPKSLFENQNITVIPSGFRVGM
  KLEAADKKSPSVICVATVTDMVDNRFLVHFDNWDESYDYWCESNSPHIHPVGWCKEHRRTLITPPGYSHV
  KHFSWDKYLEETNSLPAPARAFKVKPPHGFQKKMKLEAVDKRNPLFIRVATVADTDDHRIKVHFDGWSSC
  YDYWIDADSPDIHPVGWCSKTGHPLQAPLSPAELMEPSETGGCPTLGCRGVGHFKKSRYLGTQSGANCPY
  SEINLSKERIFPDRLSGDTSPPTTPSFPRSKRMDTRESSSSPETREKHANNFKEDSEKKKENEVKTSAEA
  KVVREEPTPSVQQSQPPQQVQQVQHAQPPQQAQKAPQAQQAQQAQQAQQAPQAPQTPQPQQAPQVQQAQQ
  APQAQQAQQPQQAQQPQQAPPVQQPQQVQQAQPTQQQAQTQQQAQRRSAVFLSFKPPIPCLPLRWEQQSK
  LLPTVAGIPASRVSKWSTDEVSEFIQSLPGCEEHGKVFKDEQIDGEAFLLMTQTDIVKIMSIKLGPALKI
  FNSILMFKAAEKNSHNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082309
• ORF Size: 2574 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082309, AAH82309
• RefSeq Size: 4280 bp
• RefSeq ORF: 2576 bp
• Locus ID: 237339
• MW: 96.6 kDa