Hectd3 (NM_175244) Mouse Tagged ORF Clone

CAT#: MR210986

Hectd3 (Myc-DDK-tagged) - Mouse HECT domain containing 3 (Hectd3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hectd3
• Synonyms: 1700064K09Rik; AI467540; AW743312
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210986 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCCTGGCCCCGGCGCGGCGCTTGAGTCCCCGCGGCAGCTCCTGGGTCGCGTGCGCTTCCTGG
  CGGAGGCGGCGCGGAGCCTCCGGGCCGGGCTGCCACTGCCTGCGGCGCTAGCTTTCGTGCCGCGGGAAGT
  GCTCTACAAGCTGTACAAGGACCCGGCGGGACCCTCGCGCGTGCTGCTGCCCGTGTGGGAAGCAGAGGGC
  CTGGGGCTGCGTGTGGGAGCCGTGGGCGCTGCCCCGGGCACAGGCTCTGGACCCCTGCGCGCCGCCCGTG
  ACAGTATCGAGCTCCGGCGCGGCGCCTGTGTGCGCACCACTGGCGAGGAGCTGTGCAACGGCCACGGGCT
  CTGGGTGAAGTTGACCAAGGAGCAGCTGGCCGAACACCTGAGTGACTGTAGTCTGGACGAAGGCTGGCTT
  CTAGTGTGTCGACCAGCAGAGGGCGGAGCCCGCCTTGTACCTATTGACACTCCCGACCACCTGCAGCGGC
  AGCAGCAGCTATTTGGCGTGGACTACCGGCCGGTGCTCAGATGGGAACAGGTAGTGGACCTGACCTATTC
  ACATCGCCTGGGATCCAGGCCTCAGCCAGCAGAGGCATATACAGAAGCTATACAAAGGCTACTCTATGTG
  CCCCCAACATGGACATATGAGTGCGACGAGGACCTGATCCACTTCTTATATGACCACCTGGGCAAAGAAG
  ATGAGAACCTGGGTAGTGTGAAGCAGTATGTGGAGAGCATAGATGTTTCTTCCTATACGGAGGAGTTCAA
  TGTGTCCTGCCTGACAGATAGCAACGCAGATACCTACTGGGAGAGTGATGGGTCCCAGTGCCAACACTGG
  GTACGGCTTACCATGAAGAAAGGCACAATTGTCAAGAAGCTGCTACTCACAGTGGATACCACAGATGACA
  ACTTCATGCCGAAACGGGTAGTAGTCTATGGGGGTGAAGGGGACAACCTGAAGAAGCTGAGTGACGTGAA
  CATTGATGAGACCCTGATCGGGGATGTCTGTGTATTAGAGGACATGACCGTCCACCTTCCAATCATTGAG
  ATCCGAATCGTTGAGTGTCGAGATGATGGGATCGATGTCCGTCTTCGAGGGGTAAAGATCAAGTCATCTA
  GACAGCGGGAGCTGGGGCTGAATGCAGACCTGTTCCAGCCGGCCAGTCTGGTGCGATATCCACGCCTGGA
  AGGCACTGACCCAGAAGTACTATACCGCAGAGCTGTCCTTCTGCAGAGATTCATCAAAATTCTGGACAGT
  GTCCTGCACCACCTAGTACCTGCATGGGACCACACCCTGGGCACCTTTAGTGAGATTAAGCAAGTGAAAC
  AGTTCCTACTGTTGTCACGCCAGCGGCCCAGCCTGGTAGCACAGTGCCTGCGTGATTCAGAAAGCAGCAA
  GCCCAGCTTCATGCCGAGGCTGTACATCAACCGCCGCCTTGCCATGGAACACCGTGCCTGCCCTTCTCGG
  GACCCTGCCTGCAAGAATGCAGTCTTCACCCAGGTTTATGAAGGTCTCAAGCCTTCTGACAAGTATGAGA
  AGCCCTTGGACTACAGGTGGCCCATGCGCTATGACCAGTGGTGGGAATGTAAATTCATTGCGGAAGGGAT
  CATTGACCAAGGAGGTGGCTTCCGTGACAGCCTGGCAGATATGTCAGAAGAACTGTGCCCCAGCTCAGCT
  GACACCCCTGTGCCTCTGCCCTTCTTTGTGCGCACAGCGAACCAGGGCAATGGCACTGGTGAGGCCCGGG
  ACATGTATGTGCCAAATCCCTCTTGCCGAGACTTTGCCAAGTATGAGTGGATTGGACAGTTGATGGGGGC
  TGCCCTTCGGGGTAAGGAGTTCCTGGTCCTGGCCCTGCCTGGGTTTGTGTGGAAGCAGCTGTCTGGAGAG
  GAAGTGAGCTGGAGCAAGGACTTCCCAGCTGTGGACTCCGTGCTGGTGAAGCTCCTGGAAGTGATGGAAG
  GAGTGGACAAGGAGACGTTTGAGTTCAAGTTTGGGAAGGAGCTGACATTCACCACCGTCCTGAGTGACCA
  ACAGGTCGTGGAGCTGATTCCTGGGGGCACGGGCATCGTGGTGGAATATGAGGATCGCTCCCGTTTCATC
  CAGCTGGTGCGGAAGGCGCGGCTAGAGGAGAGCAAGGAGCAGGTGGCAGCCATGCAGGCAGGTCTGCTGA
  AGGTGGTACCACAGGCTGTGCTGGACTTGCTGACCTGGCAAGAGTTGGAAAAGAAGGTGTGTGGGGACCC
  AGAGGTCACTGTGGATGCTCTGCGCAAGCTCACTCGGTTTGAGGACTTCGAACCATCTGACACACGCGTG
  CAGTACTTTTGGGAGGCTCTGAACAATTTCACCAATGAGGACCGGAGCCGTTTCCTGCGCTTTGTCACCG
  GCCGCAGCCGCCTCCCTGCTCGGATCTACATTTACCCAGACAAACTGGGCTATGAGACCACAGACGCCCT
  GCCTGAGTCTTCTACCTGCTCCAGCACGCTCTTCCTCCCACACTATGCCAGTGCCAAGGTATGCGAGGAG
  AAGCTCCGCTACGCCGCGTACAACTGTGTGGCCATCGACACGGACATGAGTCCCTGGGAGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210986 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGPGPGAALESPRQLLGRVRFLAEAARSLRAGLPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEG
  LGLRVGAVGAAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLSDCSLDEGWL
  LVCRPAEGGARLVPIDTPDHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYTEAIQRLLYV
  PPTWTYECDEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHW
  VRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVNIDETLIGDVCVLEDMTVHLPIIE
  IRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPASLVRYPRLEGTDPEVLYRRAVLLQRFIKILDS
  VLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPSLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSR
  DPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSA
  DTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGE
  EVSWSKDFPAVDSVLVKLLEVMEGVDKETFEFKFGKELTFTTVLSDQQVVELIPGGTGIVVEYEDRSRFI
  QLVRKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDTRV
  QYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEE
  KLRYAAYNCVAIDTDMSPWEE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175244
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175244.1, NM_175244.2, NM_175244.3, NP_780453.1
• RefSeq Size: 4582 bp
• RefSeq ORF: 2586 bp
• Locus ID: 76608
• MW: 97.3 kDa