Eif4g2 (NM_001040131) Mouse Tagged ORF Clone

CAT#: MR211011

Eif4g2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4g2
• Synonyms: DAP-5; E130105L11Rik; Nat1; Natm1; p97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211011 representing NM_001040131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG
  GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC
  TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA
  AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG
  ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT
  GATTGTGGACAAAGCCTTAGAAGAGCCAAAGTATAGCTCGCTGTATGCTCAGCTATGTCTGCGATTGGCA
  GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA
  GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA
  GCGTGAAAATCCCCTCCTTCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC
  ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA
  CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT
  AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA
  ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACTGTAGAGT
  TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG
  TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC
  CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTTGCTGATATGT
  TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT
  GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGGCACATCATGCCTCCCACGCAATCGCAG
  TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGA
  ATAAAAATCAGGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCAC
  TCAAACACCGCCTCTGGGACAGACACCTCAACTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAG
  CCTGCCAAGACTAGCAAAAAGCCACCACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCCGTTGTGA
  CTGACTATCTGAACAGTGGAAATGCCAACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACA
  CTTTCTTCCTGAGATGCTAAGCAAAGTGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAA
  GCAAGCTCTTTAATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCC
  TGAATGTATTGGAGCAGTGCCCCAAACTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTT
  TGCAGCTCGTGCTATAATTTCAGAGTTGGTGAGCATTTCCGAACTAGCTCAACCACTGGAGAGTGGCACC
  CACTTCCCTCTCTTCTTACTTTGTCTTCAACAATTAGCTAAATTGCAAGACCGAGAGTGGTTAACCGAAC
  TTTTTCAACAAAGCAAGGTCAATATGCAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTT
  GGAGATTTTGGAAGGAAAGGGACTGAGTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAG
  CAAATTAAGCTGGATCCATCCCCTCAAACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTC
  ATGTAGATAAAGGATTCGTGAACATCTTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTAAGCCC
  ACCCAGCGATGAAACAGATTCTTCCTCTGCTCCTTCCAAAGAGCAGTTAGAGCAGGAAAAACAGCTGCTG
  CTCTCTTTTAAGCCAGTGATGCAGAAATTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATG
  CTTTGCAGGTGCACTGTTACAACAGCAGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCACTTCTA
  TGACATGGAAATTATTGAAGAGGAAGCTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGA
  AAAGGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAG
  AGGAAGAAGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211011 representing NM_001040131
  Red=Cloning site Green=Tags(s)
  
  VESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANE
  KERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLA
  EDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGN
  IKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFAR
  MCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGRNDFF
  LEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQ
  FGEMGGKFMKSQISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEK
  PAKTSKKPPPSKEELLKLTEAVVTDYLNSGNANDAVSGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEK
  ASSLISLLKQEGIATSDNFMQAFLNVLEQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGT
  HFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLK
  QIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVSPPSDETDSSSAPSKEQLEQEKQLL
  LSFKPVMQKFLHDHVDLQVSALYALQVHCYNSSFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPG
  KGKALFQVNQWLTWLETAEEEESEEEAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040131
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001040131.1, NM_001040131.2, NP_001035221.1
• RefSeq Size: 7646
• RefSeq ORF: 2607
• Locus ID: 13690
• MW: 98.3 kDa
• Gene Summary: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]