Eif4g2 (NM_001040131) Mouse Untagged Clone

CAT#: MC222254

Eif4g2 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif4g2
• Synonyms: DAP-5; E130105L11Rik; Nat1; Natm1; p97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222254 representing NM_001040131
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG
  GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC
  TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA
  AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG
  ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT
  GATTGTGGACAAAGCCTTAGAAGAGCCAAAGTATAGCTCGCTGTATGCTCAGCTATGTCTGCGATTGGCA
  GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA
  GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA
  GCGTGAAAATCCCCTCCTTCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC
  ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA
  CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT
  AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA
  ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACTGTAGAGT
  TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG
  TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC
  CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTTGCTGATATGT
  TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT
  GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGGCACATCATGCCTCCCACGCAATCGCAG
  TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGA
  ATAAAAATCAGGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCAC
  TCAAACACCGCCTCTGGGACAGACACCTCAACTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAG
  CCTGCCAAGACTAGCAAAAAGCCACCACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCCGTTGTGA
  CTGACTATCTGAACAGTGGAAATGCCAACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACA
  CTTTCTTCCTGAGATGCTAAGCAAAGTGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAA
  GCAAGCTCTTTAATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCC
  TGAATGTATTGGAGCAGTGCCCCAAACTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTT
  TGCAGCTCGTGCTATAATTTCAGAGTTGGTGAGCATTTCCGAACTAGCTCAACCACTGGAGAGTGGCACC
  CACTTCCCTCTCTTCTTACTTTGTCTTCAACAATTAGCTAAATTGCAAGACCGAGAGTGGTTAACCGAAC
  TTTTTCAACAAAGCAAGGTCAATATGCAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTT
  GGAGATTTTGGAAGGAAAGGGACTGAGTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAG
  CAAATTAAGCTGGATCCATCCCCTCAAACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTC
  ATGTAGATAAAGGATTCGTGAACATCTTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTAAGCCC
  ACCCAGCGATGAAACAGATTCTTCCTCTGCTCCTTCCAAAGAGCAGTTAGAGCAGGAAAAACAGCTGCTG
  CTCTCTTTTAAGCCAGTGATGCAGAAATTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATG
  CTTTGCAGGTGCACTGTTACAACAGCAGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCACTTCTA
  TGACATGGAAATTATTGAAGAGGAAGCTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGA
  AAAGGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAG
  AGGAAGAAGCTGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040131
• ORF Size: 2607 bp
• Insert Size: 2607
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001040131.2, NP_001035221.1
• RefSeq Size: 7646
• RefSeq ORF: 2607
• Locus ID: 13690
• Gene Summary: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.