Rapgef3 (NM_144850) Mouse Tagged ORF Clone

CAT#: MR211040

Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rapgef3
• Synonyms: 2310016P22Rik; 9330170P05Rik; Epac; Epac1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211040 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTGAAGAGAATGCACCGTCCCCGCTGCTGCTCTTACCAGCTAGTGTTCGAGCACCGGCGCCCCA
  GCTGCATCCAGGGACTCCGCTGGACACCACTTACCAACAGCGAGGACTCCCTGGATTTCAGAGTGAGTCT
  GGAGCAGGCCACCACAGAGCATGTGCACAAGGCAGGGAAGCTCCTGCACCGCCATCTCTTGGCCACGTAC
  CCTACCCTCATCCGAGACAGAAAATACCATCTGCGACTATATCGGCATTGCTGCTCTGGCCGGGAGCTAG
  TGGATGGGATCTTGGCTCTGGGGCTTGGGGTCCACTCACGGAGCCAAGCCGTGGGCATCTGCCAAGTGTT
  GCTGGATGAGGGTGCCCTTTGTCATGTAAAACATGACTGGACCTTCCAGGACCGAGATGCCCAATTCTAC
  AGGTTCCCTGGACCGGAGCCCGAGCCTACAGGAACTCAAGATGTGGAAGAGGAGCTTGTTGAGGCTATGG
  CCCTCCTGTCCCAGCGAGGGCCTGATGCCCTACTCACCGTGGCGCTCCGGAAGCCCCCAGGTCAGCGTAC
  GGATGAAGAACTGGACCTGATCTTTGAAGAGCTGCTGCATATCAAGGCGGTGGCACACCTTTCTAACTCG
  GTGAAGCGGGAACTAGCTGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGAACTGTGTTGTTCAGCC
  AGGGGGACAAGGGTACCTCGTGGTACATTATCTGGAAGGGATCTGTCAATGTGGTGACCCATGGCAAGGG
  GCTGGTGACCACGTTGCACGAGGGAGATGACTTTGGACAGCTGGCTCTGGTGAACGACGCACCTCGGGCA
  GCCACCATCATCCTTCGAGAAAATAACTGTCACTTTCTGCGTGTGGACAAGCAGGACTTCAACCGCATCA
  TCAAGGATGTGGAAGCAAAAACCATGAGACTGGAAGAACACGGCAAAGTGGTCTTAGTTCTGGAGAGAAC
  CTCTCAGGGTGCTGGCCCTTCCCGTCCCCCGACCCCAGGCAGGAACCGGTATACGGTCATGTCTGGCACC
  CCAGAGAAAATCCTAGAACTGCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAGAGA
  CGTTCCTCAGTGACTTCCTGCTGACCCACAGTGTCTTCATGCCCAGCACCCAGCTCTTCACTGCCCTCCT
  GCACCACTTCCACGTGGAGCCAGCAGACCCTGCTGGAGGCAGCGAGCAGGAGCACAGCACCTACATCTGC
  AACAAGAGGCAGCAGATCCTGCGGCTAGTTGGCCGATGGGTGGCCCTGTATAGCCCGATGCTCCACTCGG
  ATCCCGTGGCCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGAGCAGAGATGCCCGACTTAGCAACTT
  GCTGAGGGAACAGTATCCAGAGAGACGGCGACACCACAGGTTGGAAAATGGCTGTGGGAACGTATCTCCT
  CAGACCAAGGCCCGGAATGCACCTGTTTGGCTCCCTAACCAGGAGGAACCCCTCCCAAGCAGCGCGGGTG
  CCATCCGAGTTGGGGACAAAGTCCCCTACGACATCTGCAGACCTGACCACTCAGTGCTGACCCTGCACCT
  GCCGGTGACGGCCTCGGTGAGAGAAGTGATGGCAGCTTTGGCCCATGAGGACCACTGGACCAAGGGACAG
  GTGTTGGTGAAGGTCAATTCTGCCGGTGATGTCGTTGGCTTGCAGCCAGATGCCCGTGGTGTGGCCACAT
  CCCTGGGGCTCAACGAGCGGCTCTTTGTTGTCGACCCACAGGAAGTGCATGAGCTGACCCCACACCCTGA
  GCAGCTGGGCCCCACTCTGGGTTCTTCTGAGATGCTGGACCTAGTGAGCGCCAAGGACCTGGCAGGCCAG
  CTGACCGACCATGACTGGAACCTCTTCAACAGGATCCACCAGGTGCAGGAGCACCTGCGGGATGTCACCA
  CCGCGAACCTGGAGCGCTTCATGCGCCGCTTCAACGAGCTGCAGTACTGGGTGGCCACAGAGCTCTGTCT
  CTGCCCTGTTCCTGGCTCCCGGGCTCAGCTACTCAGGAAGTTCATCAAGCTGGCAGCCCACCTCAAGGAG
  CAGAAGAACCTCAACTCTTTCTTTGCGGTCATGTTTGGCCTCAGCAACTCGGCCATCAGCCGGCTGGCCC
  ACACCTGGGAGCGCCTGCCCCATAAAGTACGGAAGCTGTACTCAGCCCTGGAAAGGTTGCTGGACCCTTC
  CTGGAACCACCGAGTGTACCGATTGGCTCTCACCAAGCTCTCCCCTCCTGTCATCCCCTTCATGCCCCTG
  CTGCTCAAAGACGTGACCTTCATCCATGAGGGGAACCACACACTGGTAGAGAACCTCATCAACTTTGAGA
  AGATGCGGATGATGGCCAGAGCGGTGCGGATGCTTCACCACTGCCGAAGTCACAGTACCGCGCCTCTATC
  ACCACTCAGAAGCCGAGTGTCCCACATCCACGAGGACAGCCAGGGATCAAGGATCTCCACGTGTTCTGAG
  CAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGAAGGTCATTGACAACC
  AGCGGGAACTGTCCCGCCTCTCCCGGGAGCTGGAACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211040 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEDSLDFRVSLEQATTEHVHKAGKLLHRHLLATY
  PTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFY
  RFPGPEPEPTGTQDVEEELVEAMALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNS
  VKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA
  ATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGT
  PEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPSTQLFTALLHHFHVEPADPAGGSEQEHSTYIC
  NKRQQILRLVGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQYPERRRHHRLENGCGNVSP
  QTKARNAPVWLPNQEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQ
  VLVKVNSAGDVVGLQPDARGVATSLGLNERLFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQ
  LTDHDWNLFNRIHQVQEHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQLLRKFIKLAAHLKE
  QKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPL
  LLKDVTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQGSRISTCSE
  QSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144850
• ORF Size: 2631 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144850.1, NP_659099.1
• RefSeq Size: 3801 bp
• RefSeq ORF: 2757 bp
• Locus ID: 223864
• MW: 99.2 kDa