Manba (NM_027288) Mouse Tagged ORF Clone

CAT#: MR211048

Manba (Myc-DDK-tagged) - Mouse mannosidase, beta A, lysosomal (Manba)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Manba
• Synonyms: 2410030O07Rik; B930014J03Rik; Bmn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211048 representing NM_027288
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTTCACCTGCTCTTGATATTGGCGCTATTTCGGGCCGGCTGCGTGGTGGCAGGTCCTAGCTACA
  GCCTGAGCGGGAGCTGGCGGGTGAGCAACGGGAATGGCTCCCTGGAGCTACCGGCGACTGTCCCCGGCTA
  TGTGCACAGCGCCCTGCACCAGCACGGGCTGATCCAGGATCCTTACTACAGATTTAATGACCTGAACTAT
  AGATGGATTTCCTTAGATAACTGGACCTACAGCACGGAATTTAAAATCCCCTTTAATCTCAGTGAATGGC
  AGAAGGTAAAGTTGATCTTTGATGGAGTTGACACGGTTGCAGAAATCCTGTTCAACAATGTCACCATTGG
  GAAGACAGACAACATGTTCACTGGATACAGCTTTGACGTCACCAACGTAGTCAAAGACGTGAACTCCTTA
  AAGCTGCAGTTCCGGTCAGCAGTGCAGTATGCAGAGTGTCAGAGCAAGGCTCACACTAGCTACCGGGTGC
  CCCCAGAGTGCCCCCCGGTGGAGCAGAAGGGTGAATGTCACGTCAACTTCATCCGAAAGGCACAGTGTTC
  CTTCAGCTGGGACTGGGGCCCTTCCTTCCCCTCTCAGGGAATCTGGAAAGATGTTAGAATCGAAGCCTAT
  AACATTGCTCATCTGGATTACCTCACGTTTTTGCCAGTATATGATAACGCTTCTCAGGCCTGGAATATTG
  AAATAGAGGCTTCTTTTGATGTCGCCAGCTCGAAGTCCGTTAGTGGTCAGGTGACAGTAGCCATCCCTCA
  ACTGAAAACACAGCAGACAAACGACATTGAACTTCAACAAGAGCAAAGGATTGTTAAACTACTGGTCAAA
  ATTAGGAAGGACGTTGCTGTGGAGACCTGGTGGCCTCGTGGACATGGGAACCAGACTGGGTACAACATGA
  CAATTCTCTTTGCTCTGGATGGAGGCTTAAAAATTGAGAAAGCAGCCAAGGTTTATTTCAGGACAGTGCA
  ACTGATAGAAGAGGGCATAAAAGGGTCTCCTGGTCTGAGTTTCTACTTCAAAATTAATGGGCTTCCTATA
  TTTCTGAAAGGCTCAAATTGGATTCCAGCCGACTCCTTCCAAGACAAAGTCACTTCCGATCGGCTGCAGC
  TCCTTTTCCAGTCCGTCGTGGATGCGAACATGAACACTCTGCGCGTGTGGGGGGGTGGCATCTACGAGCA
  GGATGAGTTCTATGCGCTCTGTGATGAACTTGGGATAATGGTGTGGCAGGACTTTATGTTTGCCAGTGCC
  CTTTATCCAACTGAGCCTGGCTTCTTAGCCTCCGTGAGGAAAGAAGTCACCTACCAGGTCAGGAGACTGA
  AATCTCACCCCTCCATCATCATATGGAGTGGTAATAATGAGAATGAAGTGGCTCTCAGCGTGAACTGGTT
  CCACGTAAATCCCAGGGACATGAAAACGTACATTGACGACTACGTGACCCTCTATGTGAAGAACATCAGG
  AAGATTGTCTTATCAGAAGACAAGAGCCGTCCTTTCATTGCATCCAGCCCAACCAACGGGATGAAAACCA
  TGGAGGAAGGCTGGATCTCTTACGACCCTTATAGCATCCAGTATGGTGATATTCATTTTTATAACTATGC
  CGATGACTGCTGGAATTGGAAGATCTTCCCAAAAGCTCGATTAGTGTCTGAATATGGCTACCAGTCCTGG
  CCTTCTTTTAGCACACTAGAAAAGGTCTCATCTCAGGAGGACTGGGCTTACAACAGCCGCTTCTCCCTTC
  ATCGGCAGCATCATGAAGATGGCAACCATCAGATGCTGCACCAGGTCAAGATGCATTTCAAACTCCCCCA
  GGGGACAGACCCATTGCGCACATTTAAAGACACTATCTACCTTACTCAGGTGATGCAAGCCCAGTGCATC
  AAAACAGAAACCGAGTTCTACCTGCGCAGCCGCAGCGAGATAGTGGACGGAAAAGGCCACACCATGGGGG
  CACTGTACTGGCAGCTGAACGATATCTGGCAGGCTCCTTCCTGGGCCTCTCTTGAGTACGGAGGGAAATG
  GAAAATGCTGCATTACTTCGCTCGGCGTTTCTTCGCTCCACTGTTGCCAGTGGGATTTGAAGATGAAGGT
  GTTTTTTACGTCTATGGCGTCTCAGATCTTCACAAAGACCACCACACGCAGCTCACCGTGAGGCTCCATC
  ACTGGAGCTCCCCGAAGCCTTTGTGCTCCCTCGTGAATTCAAGTATTGTGGTTAAGGCCGGAGAGGCAGT
  GGTTCTGTTCCAGATGCCGGTGTCTGAGCTGCTGAAGAGATGCAGGGGATGCACGAGGGAGACATGCGTG
  GTCTCCTTTTACTTTTCAACTGACAAAGAACTCTTCAGCCCAACCAACTACCACTTCCTGTCCTCCCTGA
  AGGACGCTAAAGGGCTGCTCGAGGCCAACATCACTGTCAACATCTCTCAGAAGGGCAATGTTTTTGTTTT
  TGATCTGGAAACGTCTGCGGTCGCTCCCTTTGTTTGGTTGGATGTAGGAAGTATCCCCGGGAGATTTAGT
  GATAACGGCTTCCTCATGATTCGGAAGAAACTCTCGGTACTGTTTTACCCTTGGAAGCCCACCAGCAAGA
  GTGAGCTGCAGCAGGCCTTCAGCGTCACCTCCCTGACGGATACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211048 representing NM_027288
  Red=Cloning site Green=Tags(s)
  
  MHLHLLLILALFRAGCVVAGPSYSLSGSWRVSNGNGSLELPATVPGYVHSALHQHGLIQDPYYRFNDLNY
  RWISLDNWTYSTEFKIPFNLSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTGYSFDVTNVVKDVNSL
  KLQFRSAVQYAECQSKAHTSYRVPPECPPVEQKGECHVNFIRKAQCSFSWDWGPSFPSQGIWKDVRIEAY
  NIAHLDYLTFLPVYDNASQAWNIEIEASFDVASSKSVSGQVTVAIPQLKTQQTNDIELQQEQRIVKLLVK
  IRKDVAVETWWPRGHGNQTGYNMTILFALDGGLKIEKAAKVYFRTVQLIEEGIKGSPGLSFYFKINGLPI
  FLKGSNWIPADSFQDKVTSDRLQLLFQSVVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASA
  LYPTEPGFLASVRKEVTYQVRRLKSHPSIIIWSGNNENEVALSVNWFHVNPRDMKTYIDDYVTLYVKNIR
  KIVLSEDKSRPFIASSPTNGMKTMEEGWISYDPYSIQYGDIHFYNYADDCWNWKIFPKARLVSEYGYQSW
  PSFSTLEKVSSQEDWAYNSRFSLHRQHHEDGNHQMLHQVKMHFKLPQGTDPLRTFKDTIYLTQVMQAQCI
  KTETEFYLRSRSEIVDGKGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFARRFFAPLLPVGFEDEG
  VFYVYGVSDLHKDHHTQLTVRLHHWSSPKPLCSLVNSSIVVKAGEAVVLFQMPVSELLKRCRGCTRETCV
  VSFYFSTDKELFSPTNYHFLSSLKDAKGLLEANITVNISQKGNVFVFDLETSAVAPFVWLDVGSIPGRFS
  DNGFLMIRKKLSVLFYPWKPTSKSELQQAFSVTSLTDTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027288
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027288.1, NM_027288.2, NM_027288.3, NP_081564.3
• RefSeq Size: 3688
• RefSeq ORF: 2640
• Locus ID: 110173
• MW: 100.8 kDa