Exoc1 (BC024678) Mouse Tagged ORF Clone

CAT#: MR211089

Exoc1 (Myc-DDK-tagged) - Mouse exocyst complex component 1 (cDNA clone MGC:30378 IMAGE:3661758)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Exoc1
• Synonyms: SEC3, Sec3p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211089 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAATCAAGCATGCGCTGCAGAGAGATATCTTCACACCAAATGATGAACGCCTGCTGAGCATCG
  TGAATGTCTGCAAAGCTGGCAAGAAGAAGAAGAACTGTTTTCTGTGTGCCACAGTGACCACTGAACGCCC
  CGTGCAGGTGAAGGTGGTCAAAGTCAAGAAATCCGACAAGGGAGATTTCTACAAAAGGCAGATTGCTTGG
  GCCCTGCGAGACCTTGCTGTGGTCGACGCCAAAGATGCCATCAAAGAAAACCCGGAATTTGATTTACATT
  TTGAGAAGGTCTATAAGTGGGTGGCCAGCAGCACGGCTGAGAAGAACGCCTTCATCTCCTGCATTTGGAA
  GCTGAACCAGCGCTATCTGCGCAAGAAAATCGACTTTGTTAATGTTAGCTCGCAGCTTTTGGAAGAATCT
  GTTCCAAGCGGAGAGAATCAGAGTGTAGCGGGAGGGGATGAAGAAGCGGTAGATGAGTACCAAGAGTTAA
  ATGCAAGAGAAGAGCAGGACATCGAGATAATGATGGAAGGCTGCGAGTGCGCCATTTCTAACGCTGAAGC
  CTTTGCAGAGAAGTTGTCCCGGGAGCTGCAGGTGCTCGATGGGGCCAACATTCAGTCAATCATGGCCTCT
  GAGAAACAAGTGAACACCCTGATGCAGTTGCTGGATGAGGCGCTAACGGAGGTGGACCAGATTGAACTGA
  AACTGAGCAGTTACGAGGAGATGCTCCAAAGTGTGAAAGAGCAGATGGATCAGATCTCTGAGAGCAACCA
  CCTGATCCATCTCAGCAACACGAATAATGTGAAGCTCCTGTCCGAGATAGAGTTCCTCGTGAACCACATG
  GACTTGGCCAAAGGTCATATAAAGGCCCTTCAGGAAGGAGATCTTGTTTCTTCCCGAGGCATCGAGGCCT
  GCACCAACGCTGCTGACGCCCTGCTGCAGTGCATGAACGTTGCTCTTCGCCCAGGCCATGACATGCTCCT
  GGCCATCAAGCAGCAGCAGCAGCGCTTCAGTGACCTGAGGGAACATTTCGCCCGGAGACTGGCCAGCCAT
  CTCAACAATGTTTTTGTTCAACAGGGTCATGATCAGAGTTCAACTCTTGCTCAGCACTCTGTTGAACTGA
  CTCTACCCAATCACCATCCATTTCATAGAGACTTGCTCCGCTATGCCAAGCTGATGGAGTGGCTAAAGAG
  TACAGACTATGGAAAATACGAGGGACTAACAAAGAATTACATGGATTATTTATCCCGACTATATGAAAGA
  GAAATCAAAGATTTCTTTGAAGTTGCAAAGATGAAGATGACTGGCACCTCTAAAGAAAGCAAGAAGTTTG
  CTACACTGCCTCGAAAAGAAAGTGCTGTCAAACAGGAAACAGAGAGTCTCCATGGAAGCTCTGGGAAACT
  CACTGGATCAACTTCTAGTCTGAATAAACTCAGTGTTCAGAGCTCGGGCAGTCGCAGGTCCCAATCGTCC
  TCCCTACTGGACATGGGGAACATGTCTGCCTCTGACCTCGATGTGGCTGACAGAACCAAATTTGATAAGA
  TCTTTGAACAGGTGCTGAGTGAACTGGAGCCACTGTGTCTGGCAGAACAGGACTTCATAAGTAAATTTTT
  CAAACTACAGCAGCATCAAAACATGTCTGCATCAATGACTGAAGCAGAAGACCTGGATGGAGGCTCATTA
  TCACGGCAGCACAGTTCTGGCACCCTGCTCCCTGTTTCCTCTGAAAAAGATATGATTCGCCAAATGATGA
  TCAAAATATTCCGTTGCATTGAACCAGAACTGAACAACCTAATTGCATTAGGAGACAAAGTAGATAGCTT
  TAACTCCCTGTACATGCTGGTCAAAATGAGTCACCACGTGTGGACCGCACAGAACGTGGACCCAGCATCT
  TTTCTAAGTACTACATTAGGAAACGTTTTGGTGACTGTGAAGAGGAACTTTGACAAATGCATTAGTAACC
  AAATAAGACAAATGGAAGAAGTAAAGATCTCAAAGAAAAGTAAAGTAGGAATTCTTCCATTTGTTGCTGA
  ATTTGAAGAATTTGCTGGGCTCGCAGAATCAATTTTCAAAAATGCTGAACGCCGTGGTGATCTGGACAAA
  GCATACACCAAATTAATCAGAGGAGTGTTCATTAATGTGGAGAAAGTAGCAAATGAGAGCCAGAAGACGC
  CCAGGGATGTGGTCATGATGGAGAACTTTCACCATATTTTTGCAACACTTTCTCGTCTGAAAATCTCATG
  TCTTGAAGCTGAGAAGAAAGAAGCCAAGCAGAAGTACACGGATCACCTTCAGTCCTACGTCATTTACTCT
  TTAGGACAGCCCCTTGAAAAGCTAAATCATTTCTTTGAAGGCGTTGAAGCTCGAGTAGCCCAGGGCATTC
  GGGAGGAGGAGGTGAGCTACCAGCTCGCGTTCAACAAGCAAGAGCTTCGCAAAGTCATTAAAGAGTATCC
  AGGCAAGGAGGTGAAGAAGGGCCTGGATAACCTCTACAAGAAGGTAGACAAGCACCTGTGTGAGGAGGAG
  AACTTACTGCAGGTGGTGTGGCACTCCATGCAAGATGAGTTTATACGCCAGTACAAGCACTTCGAGGGGT
  TGATAGCGCGCTGCTACCCTGGATCTGGAGTTACGATGGAATTCACTATTCAGGACATTCTGGATTATTG
  CTCCAGCATCGCACAGTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211089 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDKGDFYKRQIAW
  ALRDLAVVDAKDAIKENPEFDLHFEKVYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFVNVSSQLLEES
  VPSGENQSVAGGDEEAVDEYQELNAREEQDIEIMMEGCECAISNAEAFAEKLSRELQVLDGANIQSIMAS
  EKQVNTLMQLLDEALTEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHM
  DLAKGHIKALQEGDLVSSRGIEACTNAADALLQCMNVALRPGHDMLLAIKQQQQRFSDLREHFARRLASH
  LNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYER
  EIKDFFEVAKMKMTGTSKESKKFATLPRKESAVKQETESLHGSSGKLTGSTSSLNKLSVQSSGSRRSQSS
  SLLDMGNMSASDLDVADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQQHQNMSASMTEAEDLDGGSL
  SRQHSSGTLLPVSSEKDMIRQMMIKIFRCIEPELNNLIALGDKVDSFNSLYMLVKMSHHVWTAQNVDPAS
  FLSTTLGNVLVTVKRNFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDK
  AYTKLIRGVFINVEKVANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYS
  LGQPLEKLNHFFEGVEARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEE
  NLLQVVWHSMQDEFIRQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024678
• ORF Size: 2682 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024678, AAH24678
• RefSeq Size: 3292 bp
• RefSeq ORF: 2684 bp
• Locus ID: 69940
• MW: 101.9 kDa