Robo4 (BC071193) Mouse Tagged ORF Clone

CAT#: MR211107

Robo4 (Myc-DDK-tagged) - Mouse roundabout homolog 4 (Drosophila) (cDNA clone MGC:78016 IMAGE:5060473)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Robo4
• Synonyms: 1200012D01Rik|AI593217
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211107 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCTGGAGGAACGGGCCTCCTGGGGACGGAGTGGCCTCTGCCTCTGCTGCTGCTTTTCATCATGG
  TCCTCCAGGAGGACTTCCAGATCCAACCTCGGGACACAGTGGCCGTGGTGGGAGAGAGCTTGGTTCTTGA
  GTGTGGTCCTCCCTGGGGCTACCCAAAACCCTCGGTCTCATGGTGGAAAGACGGGAAACCCCTGGTCCTC
  CAGCCAGGGAGGCGCACAGTATCTGGGGATTCCCTGATGGTGTCAAGAGCAGAGAAGAATGACTCGGGGA
  CCTATATGTGTATGGCCACCAACAATGCTGGGCAACGGGAGAGCCGAGCAGCCAGGGTGTCTATCCAGGA
  ATCCCAGGACCACAAGGAACATCTAGAGCTTCTGGCTGTTCGCATTCAGCTGGAAAATGTGACCCTGCTA
  AACCCCGAACCTGTAAAAGGTCCCAAGCCTGGGCCATCCGTGTGGCTCAGCTGGAAGGTGAGCGGCCCTG
  CTGCACCTGCTGAGTCATACACAGCTCTGTTCAGGACTCAGAGGTCCCCCAGGGACCAAGGATCTCCATG
  GACAGAGGTGCTGCTGCGTGGCTTGCAGAGTGCAAAGCTTGGGGGTCTCCACTGGGGCCAAGACTATGAA
  TTCAAAGTGAGACCGTCCTCCGGCCGGGCTCGAGGCCCTGACAGCAATGTGTTGCTCCTGAGGCTGCCTG
  AACAGGTGCCCAGTGCCCCACCTCAAGGAGTGACCTTAAGATCTGGCAACGGTAGTGTCTTTGTGAGTTG
  GGCTCCACCACCTGCTGAAAGCCATAATGGTGTCATCCGTGGTTACCAGGTCTGGAGCCTGGGCAATGCC
  TCATTGCCTGCTGCCAACTGGACCGTAGTGGGTGAACAGACCCAGCTGGAGATCGCCACACGACTGCCAG
  GCTCCTATTGTGTGCAAGTGGCTGCAGTCACTGGAGCTGGTGCTGGAGAACTCAGTACCCCTGTCTGCCT
  CCTTTTAGAGCAGGCCATGGAGCAATCAGCACGAGACCCCAGGAAACATGTTCCCTGGACCCTGGAACAG
  CTGAGGGCCACCTTGAGACGACCAGAAGTCATTGCCAGTAGTGCTGTCCTACTCTGGTTGCTGCTACTAG
  GCATTACTGTGTGTATCTACAGACGACGCAAAGCTGGGGTGCACCTGGGCCCAGGTCTGTACAGATACAC
  CAGCGAGGACGCCATTCTAAAACACAGGATGGACCACAGTGACTCCCCATGGCTGGCAGACACCTGGCGT
  TCCACCTCTGGCTCTCGAGACCTGAGCAGCAGCAGCAGCCTTAGTAGTCGGCTGGGATTGGACCCTCGGG
  ACCCACTAGAGGGCAGGCGCTCCTTGATCTCCTGGGACCCTCGGAGCCCCGGTGTACCCCTGCTTCCAGA
  CACGAGCACGTTTTACGGCTCCCTCATTGCAGAGCAGCCTTCCAGCCCTCCAGTCCGGCCAAGCCCCAAG
  ACACCAGCTGCTAGGCGCTTTCCATCCAAGTTGGCTGGAACCTCCAGCCCCTGGGCTAGCTCAGATAGTC
  TCTGCAGCCGCAGGGGACTCTGTTCCCCACGCATGTCTCTGACCCCTACAGAGGCTTGGAAGGCCAAAAA
  GAAGCAGGAATTGCACCAAGCTAACAGCTCCCCACTGCTCCGGGGCAGCCACCCCATGGAAATCTGGGCC
  TGGGAGTTGGGAAGCAGAGCCTCCAAGAACCTTTCTCAAAGCCCAGGTCCAAACTCTGGTTCCCCAGGAG
  AAGCGCCCCGAGCCGTGGTATCCTGGCGTGCTGTGGGACCACAACTTCACCGCAACTCCAGTGAGCTGGC
  ATCTCGTCCACTCCCTCCAACACCCCTTTCTCTTCGTGGAGCTTCCAGTCATGACCCACAGAGCCAGTGT
  GTGGAGAAGCTCCAAGCTCCCTCCTCTGACCCACTGCCAGCAGCCCCTCTCTCCGTCCTCAACTCTTCCA
  GACCTTCCAGCCCCCAGGCCTCTTTCCTCTCCTGTCCTAGCCCATCCTCCAGCAACCTGTCCAGCTCCTC
  GCTGTCATCCTTAGAGGAGGAGGAGGATCAGGACAGCGTGCTCACCCCCGAGGAGGTAGCCCTGTGTCTG
  GAGCTCAGTGATGGGGAGGAGACACCCACGAACAGTGTATCTCCTATGCCAAGAGCTCCTTCCCCGCCAA
  CAACCTATGGCTATATCAGCATACCAACCTGCTCAGGACTGGCAGACATGGGCAGAGCTGGCGGGGGCGT
  GGGGTCTGAGGTTGGGAACTTACTGTATCCACCTCGGCCCTGCCCCACCCCTACACCCAGCGAGGGCTCC
  CTGGCCAATGGTTGGGGCTCAGCTTCTGAGGACAATGTCCCCAGCGCCAGGGCCAGCCTGGTTAGCTCTT
  CTGATGGCTCCTTCCTCGCTGATACTCACTTTGCTCGTGCCCTGGCAGTGGCTGTGGATAGCTTTGGCCT
  CAGTCTGGATCCCAGGGAAGCTGACTGTGTCTTCACTGATGCCTCATCACCTCCCTCCCCTCGGGGTGAT
  CTCTCCCTGACCCGAAGCTTCTCTCTGCCTTTGTGGGAGTGGAGGCCAGACTGGTTGGAAGATGCTGAGA
  TCAGCCACACCCAGAGGCTGGGGAGGGGGCTGCCTCCCTGGCCTCCTGATTCTAGGGCCTCTTCCCAGCG
  AAGTTGGCTAACTGGTGCTGTGCCCAAGGCTGGTGATTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211107 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSGGTGLLGTEWPLPLLLLFIMVLQEDFQIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPLVL
  QPGRRTVSGDSLMVSRAEKNDSGTYMCMATNNAGQRESRAARVSIQESQDHKEHLELLAVRIQLENVTLL
  NPEPVKGPKPGPSVWLSWKVSGPAAPAESYTALFRTQRSPRDQGSPWTEVLLRGLQSAKLGGLHWGQDYE
  FKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQGVTLRSGNGSVFVSWAPPPAESHNGVIRGYQVWSLGNA
  SLPAANWTVVGEQTQLEIATRLPGSYCVQVAAVTGAGAGELSTPVCLLLEQAMEQSARDPRKHVPWTLEQ
  LRATLRRPEVIASSAVLLWLLLLGITVCIYRRRKAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWR
  STSGSRDLSSSSSLSSRLGLDPRDPLEGRRSLISWDPRSPGVPLLPDTSTFYGSLIAEQPSSPPVRPSPK
  TPAARRFPSKLAGTSSPWASSDSLCSRRGLCSPRMSLTPTEAWKAKKKQELHQANSSPLLRGSHPMEIWA
  WELGSRASKNLSQSPGPNSGSPGEAPRAVVSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQC
  VEKLQAPSSDPLPAAPLSVLNSSRPSSPQASFLSCPSPSSSNLSSSSLSSLEEEEDQDSVLTPEEVALCL
  ELSDGEETPTNSVSPMPRAPSPPTTYGYISIPTCSGLADMGRAGGGVGSEVGNLLYPPRPCPTPTPSEGS
  LANGWGSASEDNVPSARASLVSSSDGSFLADTHFARALAVAVDSFGLSLDPREADCVFTDASSPPSPRGD
  LSLTRSFSLPLWEWRPDWLEDAEISHTQRLGRGLPPWPPDSRASSQRSWLTGAVPKAGDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC071193
• ORF Size: 2703 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC071193, AAH71193
• RefSeq Size: 3419 bp
• RefSeq ORF: 2705 bp
• Locus ID: 74144
• MW: 96.7 kDa