Aldh1l1 (BC028817) Mouse Tagged ORF Clone

CAT#: MR211113

  • TrueORF®

Aldh1l1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 1 family, member L1 (cDNA clone MGC:36396 IMAGE:5101815)


  "BC028817" in other vectors (3)


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Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Aldh1l1
Synonyms 1810048F20Rik|FDH|Fthfd|Neut2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211113 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATTGCAGTAATCGGGCAGAGCCTGTTTGGCCAGGAGGTTTACTGCCAGCTAAGGAAGGAGGGCC
ATGAGGTGGTGGGTGTGTTCACCATCCCAGACAAGGATGGGAAAGCAGATCCCCTGGGCCTGGAGGCCGA
GAAGGATGGCGTGCCCGTGTTTAAGTTCCCTCGGTGGCGGGCTCGAGGACAGGCTCTGCCCGAGGTCGTG
GCCAAGTACCAGGCTCTGGGTGCAGAACTCAACGTGCTCCCCTTCTGCAGCCAGTTCATACCCATGGAGG
TCATCAATGCCCCCCGGCATGGCTCCATCATCTACCATCCATCTCTGCTGCCCAGGCACCGAGGGGCCTC
AGCCATCAACTGGACCCTCATTCATGGAGATAAGAAAGGAGGCTTCACTATCTTCTGGGCTGATGACGGT
CTGGACACTGGTGACCTTCTGCTGCAGAAGGAGTGTGACGTGCTTCCAGATGACACCGTGAGCACGCTGT
ACAACCGTTTCCTCTTCCCAGAGGGCATCAAAGGGATGGTTCAGGCTGTGCGACTGATTGCAGAGGGCAC
AGCCCCACGCCGTCCCCAGCCTGAGGAAGGAGCCACCTATGAGGGCATTCAGAAAAAGGAGACAGCCATG
ATCAACTGGGACCAGCCAGCAGAGGCCATTCACAACTGGATCCGTGGGAATGACAAGGTGCCAGGTGCCT
GGACAGAGGCTTGTGGGCAGAAGCTGACGTTTTTCAACTCGACACTCAACACTTCAGGACTGGTGGCTCA
GGGAGAAGCTCTACCCATCCCAGGAGCCCATCGTCCAGGCCTAGTCACCAAAGCGGGACTCATCCTCTTT
GGGAATGATGATAGAATGTTGCTGGTGAAGAATATCCAGCTGGAAGATGGCAAGATGATGCCAGCCTCCC
AGTTCTTCAAGGGGTCTGCTAGCAGTGCCCTGGAGCTGACCGAGGAGGAGCTGGCCACAGCAGAGGCCGT
GCGGAGCTCTTGGATGCGAATTCTGCCCAATGTCCCAGAGGTAGAAGACTCTACAGATTTCTTCAAGTCA
GGAGCTGCATCCGTAGATGTTGTGAGGCTGGTGGAGGAGGTGAAGGAGCTGTGTGACGGGCTGGAGTTAG
AAAATGAGGATGTTTACATGGCCACCACCTTCGGGGACTTCATCCAGCTCCTAGTGAGGAAGTTGAGAGG
GGAGGACGGCGAGAGCGAGTGTGTCATTAACTACGTGGAAAAGGCAGTGAAGAAACTGACTCTCCAAATG
CCCTACCAGCTCTTCATAGGCGGCGAGTTTGTGGATGCTGAGGGCGCGAAGACCTACAGTACCATAAACC
CAACGGATGGAAGTGTCATCTGCCAGGTGTCTCTAGCTCAGGTGAGTGATGTTGACAAGGCGGTGGCAGC
AGCGAAGGAGGCCTTTGAGAATGGACTGTGGGGAAAGATAAATGCGCGTGACCGGGGCCGGCTCCTGTAC
AGGTTGGCGGACCTCATGGAGCAGCACCAGGAAGAGCTAGCCACCATTGAGGCCCTGGACGCAGGTGCCG
TCTACACGCTGGCCCTGAAGACGCATGTGGGCATGTCCATCCAGACCTTCCGATACTTCGCTGGCTGGTG
TGATAAGATCCAGGGTGCCACCATCCCCATCAACCAGGCTAGACCCAACCGCAACCTGACCTTGACCAAG
AAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAATGATGCTGTCCTGGAAGA
CAGCAGCCTGCCTGGCTGCCGGGAACACCGTGGTGATCAAGCCTGCCCAGGTGACCCCACTCACAGCCTT
GAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGC
TCGCTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGG
TGGGAAAACACATCATGAAAAGCTGTGCCCTGAGTAATGTGAAGAAGGTCTCCCTGGAGCTGGGTGGAAA
GTCACCCCTTATCATCTTTGCTGACTGTGACCTCAACAAAGCTGTGCAGATGGGCATGAGCTCCGTTTTC
TTCAACAAAGGGGAGAACTGCATTGCGGCAGGCCGGCTCTTTGTGGAGGACTCCATCCATGACCAGTTTG
TGCAGAAAGTGGTGGAGGAAGTAGGGAAGATGAAAATCGGCAACCCCCTGGACAGAGATACCAACCATGG
CCCGCAGAACCATGAGGCCCACCTGAGGAAGCTGGTGGAGTATTGCCAACGTGGTGTGAAGGAAGGGGCC
ACACTGGTCTGTGGTGGGAACCAAGTCCCAAGGCCAGGCTTCTTCTTTCAGCCAACCGTTTTCACAGACG
TGGAGGACCACATGTACATCGCTAAGGAGGAGTCCTTCGGGCCCATCATGATCATCTCTCGGTTTGCTGA
TGGGGACGTGGATGCAGTGCTATCTCGGGCCAATGCTACAGAATTTGGCCTGGCCTCTGGTGTCTTCACT
CGGGATATCAACAAGGCCCTGTATGTCAGTGACAAACTGCAGGCGGGCACTGTGTTTGTCAACACATACA
ACAAGACCGATGTGGCCGCACCTTTTGGAGGATTCAAGCAGTCTGGATTTGGCAAAGACCTGGGAGAGGC
GGCCCTGAATGAGTACCTGCGGATCAAGACTGTGACTTTTGAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211113 protein sequence
Red=Cloning site Green=Tags(s)

MKIAVIGQSLFGQEVYCQLRKEGHEVVGVFTIPDKDGKADPLGLEAEKDGVPVFKFPRWRARGQALPEVV
AKYQALGAELNVLPFCSQFIPMEVINAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFTIFWADDG
LDTGDLLLQKECDVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGTAPRRPQPEEGATYEGIQKKETAM
INWDQPAEAIHNWIRGNDKVPGAWTEACGQKLTFFNSTLNTSGLVAQGEALPIPGAHRPGLVTKAGLILF
GNDDRMLLVKNIQLEDGKMMPASQFFKGSASSALELTEEELATAEAVRSSWMRILPNVPEVEDSTDFFKS
GAASVDVVRLVEEVKELCDGLELENEDVYMATTFGDFIQLLVRKLRGEDGESECVINYVEKAVKKLTLQM
PYQLFIGGEFVDAEGAKTYSTINPTDGSVICQVSLAQVSDVDKAVAAAKEAFENGLWGKINARDRGRLLY
RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGATIPINQARPNRNLTLTK
KEPVGVCGIVIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNILPGSG
SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCALSNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF
FNKGENCIAAGRLFVEDSIHDQFVQKVVEEVGKMKIGNPLDRDTNHGPQNHEAHLRKLVEYCQRGVKEGA
TLVCGGNQVPRPGFFFQPTVFTDVEDHMYIAKEESFGPIMIISRFADGDVDAVLSRANATEFGLASGVFT
RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRIKTVTFEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028817
ORF Size 2706 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC028817, AAH28817
RefSeq Size 3045 bp
RefSeq ORF 2708 bp
Locus ID 107747
MW 98.7 kDa

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