Aldh1l1 (BC028817) Mouse Tagged ORF Clone

CAT#: MR211113

Aldh1l1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 1 family, member L1 (cDNA clone MGC:36396 IMAGE:5101815)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh1l1
• Synonyms: 1810048F20Rik|FDH|Fthfd|Neut2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211113 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTGCAGTAATCGGGCAGAGCCTGTTTGGCCAGGAGGTTTACTGCCAGCTAAGGAAGGAGGGCC
  ATGAGGTGGTGGGTGTGTTCACCATCCCAGACAAGGATGGGAAAGCAGATCCCCTGGGCCTGGAGGCCGA
  GAAGGATGGCGTGCCCGTGTTTAAGTTCCCTCGGTGGCGGGCTCGAGGACAGGCTCTGCCCGAGGTCGTG
  GCCAAGTACCAGGCTCTGGGTGCAGAACTCAACGTGCTCCCCTTCTGCAGCCAGTTCATACCCATGGAGG
  TCATCAATGCCCCCCGGCATGGCTCCATCATCTACCATCCATCTCTGCTGCCCAGGCACCGAGGGGCCTC
  AGCCATCAACTGGACCCTCATTCATGGAGATAAGAAAGGAGGCTTCACTATCTTCTGGGCTGATGACGGT
  CTGGACACTGGTGACCTTCTGCTGCAGAAGGAGTGTGACGTGCTTCCAGATGACACCGTGAGCACGCTGT
  ACAACCGTTTCCTCTTCCCAGAGGGCATCAAAGGGATGGTTCAGGCTGTGCGACTGATTGCAGAGGGCAC
  AGCCCCACGCCGTCCCCAGCCTGAGGAAGGAGCCACCTATGAGGGCATTCAGAAAAAGGAGACAGCCATG
  ATCAACTGGGACCAGCCAGCAGAGGCCATTCACAACTGGATCCGTGGGAATGACAAGGTGCCAGGTGCCT
  GGACAGAGGCTTGTGGGCAGAAGCTGACGTTTTTCAACTCGACACTCAACACTTCAGGACTGGTGGCTCA
  GGGAGAAGCTCTACCCATCCCAGGAGCCCATCGTCCAGGCCTAGTCACCAAAGCGGGACTCATCCTCTTT
  GGGAATGATGATAGAATGTTGCTGGTGAAGAATATCCAGCTGGAAGATGGCAAGATGATGCCAGCCTCCC
  AGTTCTTCAAGGGGTCTGCTAGCAGTGCCCTGGAGCTGACCGAGGAGGAGCTGGCCACAGCAGAGGCCGT
  GCGGAGCTCTTGGATGCGAATTCTGCCCAATGTCCCAGAGGTAGAAGACTCTACAGATTTCTTCAAGTCA
  GGAGCTGCATCCGTAGATGTTGTGAGGCTGGTGGAGGAGGTGAAGGAGCTGTGTGACGGGCTGGAGTTAG
  AAAATGAGGATGTTTACATGGCCACCACCTTCGGGGACTTCATCCAGCTCCTAGTGAGGAAGTTGAGAGG
  GGAGGACGGCGAGAGCGAGTGTGTCATTAACTACGTGGAAAAGGCAGTGAAGAAACTGACTCTCCAAATG
  CCCTACCAGCTCTTCATAGGCGGCGAGTTTGTGGATGCTGAGGGCGCGAAGACCTACAGTACCATAAACC
  CAACGGATGGAAGTGTCATCTGCCAGGTGTCTCTAGCTCAGGTGAGTGATGTTGACAAGGCGGTGGCAGC
  AGCGAAGGAGGCCTTTGAGAATGGACTGTGGGGAAAGATAAATGCGCGTGACCGGGGCCGGCTCCTGTAC
  AGGTTGGCGGACCTCATGGAGCAGCACCAGGAAGAGCTAGCCACCATTGAGGCCCTGGACGCAGGTGCCG
  TCTACACGCTGGCCCTGAAGACGCATGTGGGCATGTCCATCCAGACCTTCCGATACTTCGCTGGCTGGTG
  TGATAAGATCCAGGGTGCCACCATCCCCATCAACCAGGCTAGACCCAACCGCAACCTGACCTTGACCAAG
  AAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAATGATGCTGTCCTGGAAGA
  CAGCAGCCTGCCTGGCTGCCGGGAACACCGTGGTGATCAAGCCTGCCCAGGTGACCCCACTCACAGCCTT
  GAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGC
  TCGCTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGG
  TGGGAAAACACATCATGAAAAGCTGTGCCCTGAGTAATGTGAAGAAGGTCTCCCTGGAGCTGGGTGGAAA
  GTCACCCCTTATCATCTTTGCTGACTGTGACCTCAACAAAGCTGTGCAGATGGGCATGAGCTCCGTTTTC
  TTCAACAAAGGGGAGAACTGCATTGCGGCAGGCCGGCTCTTTGTGGAGGACTCCATCCATGACCAGTTTG
  TGCAGAAAGTGGTGGAGGAAGTAGGGAAGATGAAAATCGGCAACCCCCTGGACAGAGATACCAACCATGG
  CCCGCAGAACCATGAGGCCCACCTGAGGAAGCTGGTGGAGTATTGCCAACGTGGTGTGAAGGAAGGGGCC
  ACACTGGTCTGTGGTGGGAACCAAGTCCCAAGGCCAGGCTTCTTCTTTCAGCCAACCGTTTTCACAGACG
  TGGAGGACCACATGTACATCGCTAAGGAGGAGTCCTTCGGGCCCATCATGATCATCTCTCGGTTTGCTGA
  TGGGGACGTGGATGCAGTGCTATCTCGGGCCAATGCTACAGAATTTGGCCTGGCCTCTGGTGTCTTCACT
  CGGGATATCAACAAGGCCCTGTATGTCAGTGACAAACTGCAGGCGGGCACTGTGTTTGTCAACACATACA
  ACAAGACCGATGTGGCCGCACCTTTTGGAGGATTCAAGCAGTCTGGATTTGGCAAAGACCTGGGAGAGGC
  GGCCCTGAATGAGTACCTGCGGATCAAGACTGTGACTTTTGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211113 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKIAVIGQSLFGQEVYCQLRKEGHEVVGVFTIPDKDGKADPLGLEAEKDGVPVFKFPRWRARGQALPEVV
  AKYQALGAELNVLPFCSQFIPMEVINAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFTIFWADDG
  LDTGDLLLQKECDVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGTAPRRPQPEEGATYEGIQKKETAM
  INWDQPAEAIHNWIRGNDKVPGAWTEACGQKLTFFNSTLNTSGLVAQGEALPIPGAHRPGLVTKAGLILF
  GNDDRMLLVKNIQLEDGKMMPASQFFKGSASSALELTEEELATAEAVRSSWMRILPNVPEVEDSTDFFKS
  GAASVDVVRLVEEVKELCDGLELENEDVYMATTFGDFIQLLVRKLRGEDGESECVINYVEKAVKKLTLQM
  PYQLFIGGEFVDAEGAKTYSTINPTDGSVICQVSLAQVSDVDKAVAAAKEAFENGLWGKINARDRGRLLY
  RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGATIPINQARPNRNLTLTK
  KEPVGVCGIVIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNILPGSG
  SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCALSNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF
  FNKGENCIAAGRLFVEDSIHDQFVQKVVEEVGKMKIGNPLDRDTNHGPQNHEAHLRKLVEYCQRGVKEGA
  TLVCGGNQVPRPGFFFQPTVFTDVEDHMYIAKEESFGPIMIISRFADGDVDAVLSRANATEFGLASGVFT
  RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRIKTVTFEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC028817
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028817, AAH28817
• RefSeq Size: 3045 bp
• RefSeq ORF: 2708 bp
• Locus ID: 107747
• MW: 98.7 kDa