Dapk1 (BC060161) Mouse Tagged ORF Clone

CAT#: MR211125

Dapk1 (Myc-DDK-tagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dapk1
• Synonyms: DAP-Kinase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211125 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTGTTCAGGCAGGAAAACGTGGACGACTACTACGACACCGGCGAGGAACTGGGCAGTGGACAGT
  TCGCAGTTGTGAAGAAATGTCGTGAGAAAAGTACCGGTCTTCAGTATGCGGCCAAGTTCATCAAGAAAAG
  GAGGACCAAGTCCAGCCGGCGGGGCGTGAGCCGGGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG
  ATCCGGCACCCAAATGTCATCACCCTGCATGAGGTCTATGAGAACAAGACAGATGTCATTCTGATCCTGG
  AGCTTGTTGCAGGAGGTGAGCTGTTTGACTTCCTGGCTGAGAAGGAATCTCTGACTGAAGAGGAGGCAAC
  GGAATTCCTTAAGCAGATTCTCAGCGGCGTTTACTACCTGCACTCACTGCAGATCGCTCACTTTGACCTG
  AAGCCGGAAAACATAATGCTTCTGGATAGAAATGTGCCCAAACCTCGGATCAAGATCATAGACTTTGGCT
  TGGCCCATAAAATTGACTTTGGAAATGAATTCAAAAACATATTTGGGACACCAGAGTTTGTGGCTCCGGA
  GATAGTCAACTATGAGCCCCTGGGTCTTGAGGCAGATATGTGGAGCATCGGGGTAATAACCTATATCCTC
  CTAAGTGGGGCCTCCCCTTTTCTTGGAGACACCAAGCAAGAAACATTAGCGAATGTGTCCGCTGTCAACT
  ACGACTTTGAGGAGGAATTCTTCCGGAACACCAGTACCCTTGCCAAAGATTTCATCAGAAGACTGCTGGT
  CAAGGATCCAAAGAAGAGGATGACAATCCAGGACAGTTTGCAGCACCCCTGGATCAAGCCTAAAGACACC
  CAACAAGCACTTAGTCGAAAAGCCTCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCTCGGAAAA
  AATGGAAACAATCTGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTTTTGTCCAGAAGTAA
  CATGAGTGTCGCCAGGAGTGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT
  GCCATCAATGATGACAACGTACCCGGCCTGCAGCATCTCCTGGGCTCCTTGTCCAGCTATGACGTCAACC
  AGCCCAACAAGCATGGGACACCTCCATTACTGATTGCCGCAGGCTGTGGCAACATCCAGATGTTACAGTT
  ACTCATAAAACGAGGCTCAAGGATTGACGTCCAGGATAAGGGAGGATCCAATGCCATCTACTGGGCCTCT
  CGGCATGGCCATGTGGATACTTTGAAATTTCTCAATGAGAACAAATGCCCTTTGGATGTTCAAGACAAGT
  CTGGAGAGACAGCTCTTCACGTGGCAGCCCGCTATGGCCATGCAGATGTGGTTCAACTACTGTGCAGTTT
  TGGCTCTAATCCTGATTTCCAGGACAAGGAAGAGGAAACCCCCCTGCACTGTGCTGCCTGGCATGGCTAT
  TACTCCGTGGCTAAAGCTCTTTGTGAAGTTGGCTGCAACGTGAATATCAAGAATCGGGAGGGAGAGACCC
  CATTGCTGACGGCGTCTGCCAGGGGCTATCATGACATTGTGGAGTGTCTGGCTGAACATGGAGCTGACTT
  GAATGCTTCTGACAAGGATGGACACATCGCTCTTCATCTTGCTGTGAGGCGTTGTCAGATGGAAGTCATC
  AAGACCCTCCTTGGCCATGGGTCCTTTGTGGATTTCCAGGACAGGCATGGCAACACACCCCTGCACGTGG
  CCTGCAAAGATGGAAGCGCACCTATCGTGGTGGCCCTCTGTGAAGCCAGCTGCAATCTGGACATCTCAAA
  CAAGTATGGTCGGACTCCTCTCCACCTTGCAGCCAACAACGGGATCCTAGATGTGGTCCGCTACCTCTGT
  TTGATGGGCGCCAATGTGGAGGCTCTAACCTCGGATGGAAAGACGGCCGAGGACCTCGCCAAGGCAGAAC
  AGCACGAGCATGTGGCAGGGCTCCTGGCAAGACTGCGGAAGGACACACACCGAGGACTCTTCATCCAGCA
  ACTCCGACCCACCCAGAATCTCCAGCCCAGAATCAAGCTCAAACTGTTTGGCCATTCGGGATCAGGGAAA
  TCCACCCTGGTGGAATCTCTCAAGTGTGGGCTGTTAAGGAGTTTCTTCAGAAGGCGCCGGCCCAGACTAT
  CCTCTACCAACTCCACCCGCTTCCCACCGTCACCCCTGGCTGCTAAGCCAACAGTCTCAGTGAGCATTAA
  CAACCTGTACCCCGGCTGTGAGAACGTGAGCGTAAGGAGCCGCAGCATGATGTTCGAGCCGGGCCTCACC
  AAAGGGATGCTGGAAGTGTTCGTGGCTCCGTCTCACCACCTCCACTGCTCGACTGATGACCAGTCCACCA
  AAGCCATCGACATCCAGAATGCTTATTTGAACGGAGTTGGTGATTTCAGTGTGTGGGAGTTCTCTGGAAA
  CCCTGTGTACTTCTGTTGCTATGACTACTTTGCTGCCAACGACCCCACGTCCATCCACATCATCGTTTTC
  AGTCTCGAAGAACCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCTCTGG
  TCCCAGTTGAAGAACCCATAGCATTTGGAGGCAAGCTGAAGAACCCTCTCCGAGTTGTCCTGGTGGCAAC
  ACATGCTGACATCATGAACATCCCTCGGCCTGCTGGAGGCGAGTTTGGATATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211125 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKE
  IRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDL
  KPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYIL
  LSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDT
  QQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIH
  AINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWAS
  RHGHVDTLKFLNENKCPLDVQDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGY
  YSVAKALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVI
  KTLLGHGSFVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLC
  LMGANVEALTSDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGK
  STLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLAAKPTVSVSINNLYPGCENVSVRSRSMMFEPGLT
  KGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVF
  SLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060161
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060161, AAH60161
• RefSeq Size: 5056 bp
• RefSeq ORF: 2717 bp
• Locus ID: 69635
• MW: 101.2 kDa