Mthfd1 (NM_138745) Mouse Tagged ORF Clone

CAT#: MR211246

Mthfd1 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mthfd1
• Synonyms: Dcs; E430024A07Rik; Mthfd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211246 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAGCGGGAATCCTGAACGGGAAACTGGTTTCTGCGCAAATCAGGGATCGACTGAAGAACCAGG
  TAACTCGGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTGGCGATCCTGCAGGTTGGCGACAGAGA
  TGATTCTAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAGGAGATTGGGATCAAAGCCACTCACATT
  AAATTACCAAGAACATCCACAGAGTCCGAGGTATTAAAATATGTCATCTCCCTGAATGAGGATGCCTCTG
  TGCACGGATTCATAGTGCAGCTGCCTTTAGATTCAGAGAATTCCATTAACACTGAGGCAGTCATCAATGC
  CATTGCACCCGAGAAGGATGTGGATGGGTTGACGAGCGTCAGTGCTGGGAAGCTCGCCAGAGGTGATCTA
  AATGACTGCTTCATCCCATGCACACCCAAGGGATGCTTGGAACTCATCAAAGAGGCAGGGGTGCAGATTG
  CCGGAAGGCACGCGGTGGTGGTTGGTCGAAGTAAGATAGTTGGTGCACCCATGCATGACCTCCTTCTGTG
  GAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCAATCTGGACAAGGAGGTAAATAAAGGAGAC
  ATTCTGGTGGTTGCCACAGGCCAGCCTGAGATGGTGAAGGGGGAGTGGATCAAGCCTGGGGCTGTGGTCA
  TAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAATGGAAGGAAAGTCGTGGGTGATGTGGC
  CTACGATGAGGCCAAGGAGAGGGCGAGCTTTATCACGCCTGTCCCTGGTGGCGTGGGGCCCATGACCGTG
  GCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCTACAGAAATTTAAGCCAGGGAAGTGGA
  CAATTCAGTATAACAAGCTGAACCTCAAGACTCCTGTCCCAAGTGACATTGCTATATCACGATCTTGCAA
  ACCAAAGCTCATCGGGAACTTGGCCCGAGAAATCGGGCTGCTCACCGAAGAGGTGGAGTTGTACGGGGAA
  ACGAAGGCTAAAGTCTTGCTGTCAGCATTAGATCGCCTGAAGTATCAGCCAGATGGGAAATATGTCGTGG
  TGACTGGAATTACTCCAACACCGCTGGGAGAAGGGAAGAGTACCACCACGATCGGGCTGGTGCAGGCCCT
  CGGCGCCCACCTGCGACAGAACGTCTTTGCGTGTGTGCGACAGCCTTCGCAGGGCCCCACCTTTGGGATA
  AAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCAATGGAAGAGTTTAACCTCCACCTCACTG
  GTGACATCCACGCCATCACTGCTGCTAACAACCTCGTGGCTGCTGCCATTGATGCTCGGATATTTCATGA
  GCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCTTCAGTAAACGGAATAAGAAAATTCTCT
  GACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGACTGACCCCACCACGCTGACAGATGACG
  AGATAAACAGATTTGCAAGACTGGATATTGACCCAGAAACCATAACATGGCAGAGAGTGTTGGACACCAA
  CGATAGATTCCTGAGGAAGATCACAATTGGACAGTCTCCAACAGAGAAAGGCCACACACGCACGGCCCAG
  TTTGATATCTCTGTGGCCAGTGAGATCATGGCAGTCCTGGCCCTCACGAGCTCTCTGGAAGACATGAGAG
  AGAGACTGGGCAGAATGGTGGTGGCGTCCAGTAAGAAAGGGGAGCCTATCAGTTGCGAAGACCTGGGCGT
  GAGTGGGACGCTGACGGTGCTGATGAAGGATGCAATCAAGCCCAACCTCATGCAGACCTTAGAGGGCACG
  CCAGTGTTTGTCCATGCCGGGCCTTTCGCCAACATTGCACACGGGAATTCCTCCATCATTGCAGACCGGA
  TTGCACTCAAGCTGGTAGGCCCTGAGGGCTTCGTAGTGACGGAAGCTGGATTCGGAGCAGACATAGGAAT
  GGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGCCACACGTGGTGGTTCTGGTTGCCACT
  GTCAGGGCCCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGCTGGACTACCTCTTCCCAAGGCTTACA
  CAGAAGAGGACCTGGACCTGGTTGAAAAGGGCTTCAGTAACTTGAGGAAACAAATCGAAAATGCTAGAAT
  GTTTGGAGTCCCTGTCGTCGTGGCTGTGAACGTGTTCAAGACAGATACAGATGCTGAGCTGGACCTTGTC
  AGCCGCCTCTCCAGAGAACACGGGGCTTTTGATGCTGTCAAGTGCACCCACTGGGCAGAAGGGGGCCAGG
  GGGCCTTGGCCCTGGCTCAGGCTGTCCAGAGAGCGTCACAGGCCCCCAGCAGCTTCCAGCTCCTCTATGA
  CCTCAAGCTCTCAATTGAGGATAAAATCAGGATTATTGCACAGAGGATCTACGGGGCCGATGACATCGAA
  TTGCTCCCGGAAGCACAGAACAAAGCAGAAATTTACACAAAGCAGGGCTTTGGGAATCTACCCATCTGCA
  TGGCCAAAACACACTTGTCCTTGTCTCACAACCCAGAGCAAAAAGGGGTGCCTACTGGTTTCGTTCTGCC
  CATCCGGGACATCCGTGCCAGCGTTGGGGCAGGTTTCCTGTATCCTTTAGTAGGAACGATGAGCACAATG
  CCTGGACTCCCTACGAGGCCCTGTTTTTATGATATTGATTTGGATCCTGAAACGGAACAAGTGAATGGAT
  TGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211246 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAEEIGIKATHI
  KLPRTSTESEVLKYVISLNEDASVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSVSAGKLARGDL
  NDCFIPCTPKGCLELIKEAGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDKEVNKGD
  ILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTV
  AMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGE
  TKAKVLLSALDRLKYQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGI
  KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFS
  DIQIRRLRRLGIEKTDPTTLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQ
  FDISVASEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGVSGTLTVLMKDAIKPNLMQTLEGT
  PVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVAT
  VRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLV
  SRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIE
  LLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTM
  PGLPTRPCFYDIDLDPETEQVNGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138745
• ORF Size: 2808 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138745.2
• RefSeq Size: 3324
• RefSeq ORF: 2808
• Locus ID: 108156
• MW: 101.3 kDa
• Gene Summary: This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. [provided by RefSeq, Oct 2009]