Rtn3 (NM_001003934) Mouse Tagged ORF Clone

CAT#: MR211332

Rtn3 (Myc-DDK-tagged) - Mouse reticulon 3 (Rtn3), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn3
• Synonyms: RTN3-A1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211332 representing NM_001003934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCGTCAGCGGCCACTCAGTCCCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAG
  CTCTCGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCGTG
  TGCGGATTCCTTTGTTTCTTCCTCTTCCTCTCAGCCTGTATCTATATTTTCGACCTCACAAGCGGGATTG
  AGCTCTCTTTGCTCTGATGAGCCACCTTCAAAAAGTATGACTTCCTCCTTTCTTTCATCTTCTGAAATAC
  ATAACCCTGACCCTACAACACCGCTTGGAGAAAAGAGTGAAACATTAGGTAGCCAGTTTGTTCTAGCTAA
  AGGAAAAGACCCCTTGGTTCTTCTAGATAAGAAAAAACTGGACTCACCTCAGGGGACCAACAAGGACAGA
  GTAGACGCTCCGGTTTCTCTTGCAACTGGCATTCCTTGCAGCCACCCTTCTATTCCAGACAGTTTCCCAG
  AGCAACCTGCTTTTCTGTCAAAAGAAATTGGTCCAGCAGAAGAGTGGGTAGTTAAAGACCAAGAACCCAA
  GAACCCAAACAAGGTTCCAGATGGGGAGGACAGAAGTGCACTGGATTTTGGGCAGTCTAAGGCAGAACAC
  ATTTGTACATATTCCTTGTCCCCATCTGAACTTCCAGTAGCCAGTGTAGAAAAAGATTCTCCTGAGTCAC
  CGTTTGAAGTAATTATTGACAAAGCAACATTTGACAGAGAATTTAAAGATTTGTATAAGGAAAACCCAAA
  TGATTTGGGTGGCTGGGCAGCTCATGGCGATAGAGAATCCCCCGCAGACTTGTTGGAAATGAATGACAAA
  CTCTTTCCACTGAGAAATAAAGAGGCAGGGCGTTACCCATCCTCTGTACTGCTCGGTAGACAGTTCTCAC
  ACACTACAGCAGCACTGGAAGAGGTGTCTAGATGTGTGAATGATATGCATAACTTTACTAATGAAATACT
  GACTTGGGACTTAGATCCCCAAGCAAAACAACAGGCCAATAAAACATCTTGCACCACAGAAAGTACAGGA
  CTAGACAGGAGTGAACTTCGCTCAGAAATTCCAGTTATAAATCTTAAAACAAACCCTCAACAGAAAATGC
  CTGTATGTTCTTTTAATGGGAGCACTCCCATTACCAAATCCACAGGTGACTGGACAGAAGCATTTACAGA
  AGGAAAACCTGTAAGAGACTACCTCAGTTCCACAAAAGAAGCTGGTGGCAACGGTGTGCCAGGCAGTTCT
  CAGCTTCATTCTGAGCTGCCTGGCTCTATGCCTGAGAAATGGGTCTCAGGCTCTGGAGCAGCCACAGTGG
  AAGTAACTTTACCTAACCTGAGGGGTGCCTGGCCTAACTCTGTGATGGGGGAAGTCACAGAGGTTGATAG
  TTCTGGGGAATCTGATGACACAGTAATAGAGGACATCACAGAAAAACCTGACTCCCTTCCAAGTGCTGCT
  GCAAAAACAAGTGAAAGGGAAATCAAAGAGACTCCCAGTCGTGAAACTGTGAGGAGTGAAATGTGTGAAA
  ACTCTGAGCAGCCGCAGGCCCAGCCAGAAACTCCTACTCAGAAGAGTCTGGAAGGTGAGGTGGCTTCACA
  AGTACCTAATACCCTGAATGAAGTCACACCTGAAAAGCTTGATATGACTAACAACCCCAAAGTTTGCTCA
  GCAGCACCTCCAAGTGTTCTTAATGAGACAGGATTCTCACTAACTGTGCCAGCTTCTGCCAAGTTGGAAT
  CTTTGCTTGGAAAATATGTTGAAGATACAGATGGTTCCTCCCCAGAGGACTTGATGGCTGTCCTCACAGG
  AGCCGAGGAGAAGGGGATAGTGGATAAAGAGGAAGGTGATGTTTTGGAAGCAGTGTTAGAGAAGATAGCA
  GACTTTAAAAACACTTTGCCTGTGGAACTCTTGCATGAAAGTGAGCTAAGTGGTTCTGAAACCAAAAATA
  TTAAAAGCAAATACAGTGAAGACAGCAGAGAAACAACTGGAGGTGCCCCTACGATGTCTCCCGACTTAGA
  GCAGGAGCAGCTCACCATCAGAGCCATTAAAGAATTAGGAGAAAGGCAAGCTGAGAAGGTGCAGGATGAA
  GGAATATCTTCTGGAGGAAAACTCAAGCAAACCTTTGCTCCACAGTCTGGGCCACAGAGTTCATCTGACA
  TCCTAGAACACACAGATGTCAAAACTGGATCTGATCTTGGAATTCCCAAAAATCCTACTATCATCAAAAA
  CACTAGAATAGATTCTATTTCCAGCCTTACCAAGACTGAAATGGTTAACAAGAATGTTCTAGCAAGACTT
  CTCAGTGATTTCCCAGTGCACGATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCA
  CCACACTGATCATGCTGCTCTCTCTGGCAGCCTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGC
  TCTTCTCTCTGTCACCATCAGCTTCAGAGTCTATAAGTCTGTCATTCAAGCTGTGCAGAAGTCAGAAGAA
  GGACATCCATTCAAGGCCTACTTGGATGTGGACATTACCCTGTCTTCAGAAGCTTTCCACAACTACATGA
  ATGCTGCGATGGTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGT
  TGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAATGGAATTACC
  CTTCTGATTCTCGCCGAGCTGCTTGTCTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTG
  ACCACTATGTTGGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGG
  AATCGCCAAAAAAAAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211332 representing NM_001003934
  Red=Cloning site Green=Tags(s)
  
  MAESSAATQSPSVSSSSSGAEPSALGGGGGSPGACPALGAKSCGSSCADSFVSSSSSQPVSIFSTSQAGL
  SSLCSDEPPSKSMTSSFLSSSEIHNPDPTTPLGEKSETLGSQFVLAKGKDPLVLLDKKKLDSPQGTNKDR
  VDAPVSLATGIPCSHPSIPDSFPEQPAFLSKEIGPAEEWVVKDQEPKNPNKVPDGEDRSALDFGQSKAEH
  ICTYSLSPSELPVASVEKDSPESPFEVIIDKATFDREFKDLYKENPNDLGGWAAHGDRESPADLLEMNDK
  LFPLRNKEAGRYPSSVLLGRQFSHTTAALEEVSRCVNDMHNFTNEILTWDLDPQAKQQANKTSCTTESTG
  LDRSELRSEIPVINLKTNPQQKMPVCSFNGSTPITKSTGDWTEAFTEGKPVRDYLSSTKEAGGNGVPGSS
  QLHSELPGSMPEKWVSGSGAATVEVTLPNLRGAWPNSVMGEVTEVDSSGESDDTVIEDITEKPDSLPSAA
  AKTSEREIKETPSRETVRSEMCENSEQPQAQPETPTQKSLEGEVASQVPNTLNEVTPEKLDMTNNPKVCS
  AAPPSVLNETGFSLTVPASAKLESLLGKYVEDTDGSSPEDLMAVLTGAEEKGIVDKEEGDVLEAVLEKIA
  DFKNTLPVELLHESELSGSETKNIKSKYSEDSRETTGGAPTMSPDLEQEQLTIRAIKELGERQAEKVQDE
  GISSGGKLKQTFAPQSGPQSSSDILEHTDVKTGSDLGIPKNPTIIKNTRIDSISSLTKTEMVNKNVLARL
  LSDFPVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEE
  GHPFKAYLDVDITLSSEAFHNYMNAAMVHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGIT
  LLILAELLVFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003934
• ORF Size: 2892 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003934.1, NM_001003934.2, NP_001003934.1
• RefSeq Size: 5038 bp
• RefSeq ORF: 2895 bp
• Locus ID: 20168
• Cytogenetics: 19 A
• MW: 104.3 kDa