Usp15 (NM_027604) Mouse Tagged ORF Clone

CAT#: MR211381

Usp15 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp15
• Synonyms: 4921514G19Rik; AI327321; E430033I05Rik; Gcap18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211381 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC
  TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
  TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT
  CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA
  CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT
  GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG
  AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG
  AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATGTGAGCAACAC
  ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG
  ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAAGTCCCCAGGTGCATCCA
  ATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTATAACAACATCAACAACAGAAA
  TGTGAAAAACTCAAATTATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGA
  AGAAACAATGAACAGCCAGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCA
  TTCAGTGTTTGAGCAACACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAA
  TTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGG
  TCTGGAAAGTTTAGCTACGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCT
  CTGGATATCAGCAGCAGGACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAA
  CAGAATTAGGAAAAAACCCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAA
  GAAGCCTGGGAGAACCATTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGT
  CTACGTTAGTGTGTCCTGAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCC
  ATTGCCTATGAAAAAAGAACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATG
  CAATACAAAGTTATTGTGCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGG
  GAGTACCTGCAGATAAGATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGA
  TGAAAACCTGAGTAGTATTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAA
  GACACAGAGCATGTAGTCATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACGCACCACA
  CTGGCTCTTCACTGTTCGGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTA
  TAATCTTCTACTCTTGAGAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACAC
  CTACGTTGCTGTGAGGACCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAA
  GTGAGATGGAAACCGACGAGCCAGGTGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAA
  CAGTCAGTCTGAAGATTCAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGC
  AAAGGTCAACTTACGGGACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACA
  TCAACTACATCAAAGACGACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATC
  TTTTCTCGCTTTGGACTGGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTT
  GAAAAACATGAAAGTGTGGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGC
  TCTTCACCACCAAGGAGGAGTTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCA
  AGCCACAAAGAAATTAGATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTAT
  AGTCGCTACATGAGAGACAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAAT
  TCCTAATTAACCCAAATGCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGAT
  GGGAGGAGGACATTATACTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGT
  AGCGTCTCCACTGCATCTGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGG
  ACACTTTCAGTGGGACTGGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTAT
  CCCCCTGGAAAGTGACGAAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACT
  AAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211381 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGL
  LKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCE
  NGNMNNVVTRRFSKADTIDTIEKEIRKIFNIPDEKEARLWNKYVSNTFEPLNKPDSTIQDAGLYQGQVLV
  IEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNINNRNVKNSNYCLPSYTAYKNYDYSEPG
  RNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMW
  SGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAE
  EAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPM
  QYKVIVPKIGNILDLCTALSALSGVPADKMIVTDIYNHRFHRIFAVDENLSSIMERDDIYVFEININRAE
  DTEHVVIPVCLREKFRHSSYTHHTGSSLFGQPFLMAIPRNNTEDKLYNLLLLRMCRYVKMSTETEETDGH
  LRCCEDQNINGNGPNGLHEEGSPSEMETDEPGDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEETC
  KGQLTGHKKRLFTFQFNNLGNNDINYIKDDTSHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDF
  EKHESVEYKPPKRPFVKLKDCIELFTTKEELGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSY
  SRYMRDKLDTLVDFPISDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDS
  SVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDLENENCMHT
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027604
• ORF Size: 2946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027604.2, NM_027604.3, NM_027604.4, NP_081880.2
• RefSeq Size: 11564
• RefSeq ORF: 2946
• Locus ID: 14479
• MW: 112.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Aug 2014]