Tcof1 (BC021514) Mouse Tagged ORF Clone

CAT#: MR211447

Tcof1 (Myc-DDK-tagged) - Mouse Treacher Collins Franceschetti syndrome 1, homolog (cDNA clone MGC:36035 IMAGE:5351783)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tcof1
• Synonyms: treacle
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211447 representing BC021514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTCATTTACCATCATCTGTTGCAAGCAGGCTACG
  TCCGCGCGGCGCGGGAAGTAAAGGAGCAGAGCGGCCAGAAGAGTTTCCTGACTCAGCCCGTCACCCTTCT
  GGACATCTATACACACTGGCAACAGACCTCAGAGCTTGGCCAGAAGCAGAAGGCAGAGGATGATGAGACC
  CTTCAGGCTAAGAAGTCTCGAGTGTCGGATCCTGTTAGCAGCTCAGAGAGCTCGGATCAGGAGAAGGAAG
  AGGAGGCAGCAACCGAAAGGGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCTGCAACCGCAGCTTT
  GCCATCAAAAGTAAAAGAAAAGGGAAAGACCAAGACAGCCAACAAGACGGTGAACTCTGTGTCGCACCCT
  GGGTCCGGAAAGACGGTGGTCCACCTGCTCTCTGGGAAGTCACCCAAGAAGTCAGCAGAGCCCTTGGCAA
  ACACTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAATGCCCAAGCCCTCGGACCCACTGCCAAGTCTGG
  AACGGTGTCAGCGGGCCAAGGCAGCAGTTCCAGTGAAGATTCCTCCATCTCAAGCGATGAGACAGATGTC
  GAGGTGAAATCTCCAGCAAAACCAGCCCAGGCCAAAGCTTCAGCAGCCCCTGCCAAGGATCCTCCAGCAA
  GAACAGCCCCAGGCCCTACCAAGTTAGGGAATGTGGCGCCCACACCTGCTAAACCAGCCAGGGCGGCAGC
  GGCAGCTGCTGCTGCTGCTGTGGCTGCTGCTGCTGCTGCAGCAGCAGAAGAGTCTGAGAGCAGTGAGGAG
  GACTCAGACAGTGAGGACGAGGCCCCTGCTGGTCTGCCCAGCCAGGTAAAAGCCTCTGGAAAAGGTCCCC
  ATGTCAGAGCCGACTCGGTGTCTGCCAAGGGGATCTCTGGGAAAGGGCCCATCTTAGCAACCCCAGGGAA
  GACTGGGCCTGCAGCCACCCAGGCCAAGGCAGAAAGGCCAGAGAAGGACTCGGAAACCAGCAGTGAGGAC
  GATTCTGATAGTGAGGATGAAATGCCAGTCACTGTGAATACTCCTCAGGCAAGGACTTCTGGGAAGAGCC
  CTCGGGCCAGAGGTACCTCAGCCCCCGCCAAGGAGTCATCCCAGAAAGGGGCTCCTGCAGTCACCCCTGG
  AAAGGCAAGGCCTGTGGCAGCCCAGGCAGGGAAACCAGAAGCCAAGAGCAGTGAGGAGTCAGAGAGTGAC
  AGTGGGGAGACACCAGCTGCTGCGACTCTGACCACGAGTCCTGCCAAGGTGAAACCTTTGGGGAAGAGCT
  CCCAGGTCAGACCTGTTTCCACCGTCACCCCGGGGTCATCGGGAAAAGGTGCCAACCTGCCCTGCCCTGG
  GAAGGTGGGGTCAGCAGCTCTCAGGGTCCAAATGGTAAAGAAAGAAGATGTCTCGGAGAGCAGCAGTGCA
  GAGCTGGACAGTGACGGGCCTGGGAGCCCAGCCAAGGCAAAGGCCTCCCTTGCGCTCCCTCAGAAGGTGA
  GGCCTGTGGCCACCCAGGTCAAGACTGACAGGGGCAAAGGCCACTCAGGGAGCAGTGAGGAGTCATCTGA
  CAGCGAAGAGGAGGCAGCACCAGCAGCCTCTGCTGCTCAGGCTAAGCCAGCTCTGGAAAAGCAGATGAAA
  GCTTCCTCTAGGAAAGGCACGCCTGCATCCGCAACAGGAGCGAGCACCTCGTCCCATTGTAAGGCAGGAG
  CGGTGACCTCTTCAGCCAGCCTGTCATCCCCAGCTCTGGCCAAGGGCACCCAGAGGTCAGATGTGGACTC
  TTCCAGTGAGTCTGAGTCAGAAGGAGCTGCTCCCAGCACCCCCAGGGTACAGGGGAAGTCTGGGGGCAAG
  GGCCTCCAAGGGAAAGCTGCCTTGGGGCAAGGGGTGGCCCCAGTGCACACTCAGAAGACAGGGCCTTCGG
  TCAAAGCTATGGCTCAGGAAGACTCAGAGAGCCTCGAGGAGGACTCCAGCAGCGAGGAAGAGGATGAGAC
  CCCAGCACAGGCCACGCCCTTGGGGAGACTTCCTCAGGCCAAAGCCAACCCACCTCCCACTAAGACACCT
  CCAGCGTCTGCATCTGGAAAAGCTGTGGCTGCTCCAACCAAGGGAAAACCACCTGTTCCGAACAGCACCG
  TCTCTGCAAGGGGCCAGCGGTCTGTGCCAGCCGCGGGAAAAGCAGGGGCCCCAGCAACCCAAGCCCAGAA
  GGGTCCCGTGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTAAAGAGGAGTCTGACAGTGAAGAAGAG
  ACGCCAGCCCAGATAAAACCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGAGT
  CTCCTAAAAAAGGAGCCCATCCAGGAACCCCCGGCAAGACGGGATCTTCAGCTACCCAGGCCCAGCCAGG
  GAAGACAGAGGACTCAGACAGCAGTAGTGAAGAGTCTGACAGTGACACAGAGATGCCATCAGCCCAGGAT
  GCTATCTCCCAGCCTGCCAGAGGCAAAGCGTCAGGTCCAGCGTCCCCAGAGAAGAGCATAGAAGGGTCCT
  CAGAGAGCAGTGATGAGGATCTGCCCTCCGGCCAGGCGATTAAATCCCCTCCAGTTTCTGTCAACCGTAA
  CAGTAGTCCAGCTGTCCCAGCTCCTACCCCAGAAGGAGTCCAGGCTGTGAACACCACAAAGAAGGCCTCA
  GGCACCACTGCCCAGAGCTCCTCCTCTGAGAGTGAGGACGGGGACGAGGACTTGATTCCTGCCACACAAC
  CCTCCACCTATGCTCTCAGAACCAGTGTGACGACGCCCGCAGCCCTCTCACGAGCAGCTTCCCAACCCAG
  CAAGAGTGAGCAGTCTAGCCGGATGCCAAAAGGCAAGAAAGCAAAGGCGGCGGCGTCCGCTCAGGTACTG
  TCGGACCAGCAGTGCCGTGGAAACACTCCCCATGATGCCTCCCCAGAGCGCACCCATCCAGCCCAAAGCC
  ACCAACAAGCTCGGGAAATCCAAGCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211447 representing BC021514
  Red=Cloning site Green=Tags(s)
  
  MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDET
  LQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHP
  GSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDV
  EVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEE
  DSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSED
  DSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESD
  SGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSA
  ELDSDGPGSPAKAKASLALPQKVRPVATQVKTDRGKGHSGSSEESSDSEEEAAPAASAAQAKPALEKQMK
  ASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGK
  GLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTP
  PASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEE
  TPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQD
  AISQPARGKASGPASPEKSIEGSSESSDEDLPSGQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKAS
  GTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQVL
  SDQQCRGNTPHDASPERTHPAQSHQQAREIQAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021514
• ORF Size: 3039 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC021514.1
• RefSeq Size: 4088
• RefSeq ORF: 3041
• Locus ID: 21453
• MW: 149.8 kDa
• Gene Summary: May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. May participate in certain stages of ribosome biogenesis. [UniProtKB/Swiss-Prot Function]