Slc38a10 (NM_001164800) Mouse Tagged ORF Clone

CAT#: MR211603

Slc38a10 (Myc-DDK-tagged) - Mouse solute carrier family 38, member 10 (Slc38a10), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc38a10
• Synonyms: 1810073N04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211603 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCCGCCTCCACCTCCAAGTGGGGGCTGATCACGAACGTGGTGAACAGCATCGTGGGCGTCAGCG
  TGCTCACCATGCCTTTCTGCTTCAAGCAGTGTGGCATTGTCCTGGGGGCCCTGCTCCTGGTCTTCTGCTC
  CTGGATGACACACCAGTCTTGCATGTTCTTGGTGAAGTCGGCCAGCCTGAGCAAGAGGAGGACCTATGCT
  GGCCTGGCGTTCCACGCCTACGGCAAGGCCGGGAAGATGCTGGTGGAGACCAGCATGATTGGGCTGATGC
  TGGGATCCTGCATTACCTTCTATGTCGTGATCGGTGACTTGGGGTCCAACTTCTTTGCTCCGCTGCTTGG
  ATTACAGGTGACCAGGACTGTCCGTGTGTTCCTGCTCTTCGCAGTGTCCCTGTTCATTGTGCTCCCGCTC
  AGCCTGCAGAGAAACATGATGGCCTCTATCCAGTCCTTCAGTGCCATGGCGCTCCTCTTCTACACCGTCT
  TCATGTTCGTGATTGTGCTGTCCTCCCTTAAACATGGCCTCTTTAGTGGGCAGTGGCTGCGACAGGTCAG
  CTACATTCGCTGGGAAGGTGTTTTCCGCTGTGTCCCCATCTTTGGCATGTCCTTTGCCTGTCAGTCCCAG
  GTCCTGCCCACCTATGACAGCCTGGACGAGCCATCAGTGAAGACCATGAGCTCCATCTTTGCCTCCTCCC
  TCAACGTGGTCACCGCCTTCTATGTCATGGTGGGGTTTTTTGGTTACGTCAGCTTCACTGATGCCACCAC
  AGGCAATGTGCTGATCCACTTCCCCTCCAACCCGGTGACAGAGATGATCCGAGTGGGCTTCGTGATGTCT
  GTGGCTGTGGGCTTCCCCATGATGATTCTGCCGTGCAGGCAGGCCTTGAACACACTGCTGTTTGAGCAGC
  AGCAAAAAGATGGAACCTTTGCTGCAGGAGGCTACATGCCCCCACTCCGGTTTAAAGTCCTCACCCTCTC
  GGTGGTGTTTGGAACCATGGTTGGTGGGGTCATGATCCCCAATGTGGAAACCATCCTTGGCTTCACAGGA
  GCAACGATGGGGAGCCTCATCTGCTTTATCTGCCCGGCTCTGATCTATAAGAAAGCCCACAAGAATGCCC
  CCTCAGCCCAGGTGGTGCTCTGGGTCGGCCTGGGCATCCTCGTGGTCAGCACACTCACCACCCTCTCTGT
  GACCGAAGAAGCTCCTCTGGACTTGACGCAAGAAGCTCGCAGCGGCCACCGAGGAGATGCTGAGGGCGCA
  ATGAAGGTGGAGGCAGCTCGGCTATCAGTCCAGGATCCCGTTGTAGTTGTTGCTGAGGATAGCCAAGAGA
  AGCTAAAGCCAGCAGAGGACAAAGAGGTACTGGAGCAGGCCCAGATCAAGGGTCCTGTAGATGTGCCTGG
  CGGGGAAGCTCCCAAGGAGAAGCAGGAAGCCGCACAGCTGGATCGCCCCGGCCAAGGTATTGCTGTCCCT
  ATGGGTGAGGCCCATCGCCATGAGCCTCCCATCCCTCATGATAAAGTGGTGGTGGATGAAGGCCAGGACC
  AAGAAGGGCCAGAGGAGAAAAAGCCACCTCCCAGGCTCCCAGATGAAGGAGACCCTGCAGGCAGGGGTCA
  AGGGGCACCACCTCTGCCTGAGTCAGAGAAGGAGAAGCAGGAACCTGAGAGAGGAGGGGAAGGAAAGAGA
  CCCGGGCAAGTCCTGGCAGTAGGAGAAACTGAACATCCTCAGAAGGTTCCAGAAGCAAATGGCCAGCCAC
  CTGTGCAGCCCAGGAAGGAAGATTCCAGGCCAGGAAATAGGGATCCGCAGCCAGCTGCCCAGGCCAGGGA
  CTCTGTGGAGCTGAAGGCTCTGGCAGCAGATGACGGCAGGGAGCCTGCACAGAAGGCTGGAGGCGCCCTG
  TGGAAGCCCGTGGAGAGTGCTGCTGAGAGCGATGCTGGTGGGAAGGCGGGCCTCCCTGTGCAAAGGCCGG
  AGGCTGCAGAGCAGAGGGAGAAGAAGGAGGCTGAGCAACAGGGTGGAGACCAGGCGGGGAGCAAGCTGGA
  AGAAGCTGGGAGGGCAGAGATGCTGGACCATGCCGTGCTGCTACAGGTGATCCAAGAGCAGCAGGTGCAG
  CAGAAGCGCCTCCTGGACCAGCAGGAAAAGCTGCTCGCAGTGATTGAGGAGCAGCACAAGGAGATCCGGC
  AGCAGCGACAGGAGGGCGAGGAAGACAAGCCTAAGCCTGACGTGCAGCCAGAGCCTGGGGTGGCTGTACT
  CCGAGGACAGGAGGAGGAGGCTGAGCACGCTGGGGAGACACTGGGAGATGACCCTTCACAGCCTTTGCAA
  CCCGTGCTTGGAGCTCCTAGGGGTCGCCCCGCTCCATCCCAAGACATGGGCCAGCACCTCCCAGGGGAAG
  TCAAGGTGCTGCCAGGCAGAGACCTTGCTGACCTTCCTGCTGGTGGCTCTGAAACAGAGCCCCAGGGGGC
  CCCGATTGATCTGAGAGAAGACCCGAAAGCTGCCATCAAGGCGGCTGGAGCTGGGAAGGAGCTGGTCCCA
  GGGGACTTGGAAGCAGTGCACAAGGCAGCCCCTCCTGAGGTACCCAAGAGTCCAGAGAAGCAGGTTGCCA
  AGGCAGTTGCCGGGCAGCGCCAAGATGTCTTTGGTGAAGGCTCCGAAGAAAGGAAAGAAACTGGAAAGGA
  AGCAATGGCCCCTGGTGCTGATACTCAGAAAGAGGCTGTCCAGCCCTTGGTAGGAGCAGAAGCTAAGGAC
  ACAAAATCCAGGCAGTCGGGACCCACCAAGGCCCCAGTTCAGACCCAAGCCAAGTTTCACCCAGAACCCC
  AGGCCATCTTTGACACAGGTCAGGGTTCTCACCCAGAGGTGAGAAGTGAGGCCCCCCGAGCGGTTCACAT
  ACCTCCTGAGGAGCAGCACAAAGGAAAAGGGGGTGCCGCCATCCAGGAGGCAAAGCAGAGACCAGATCCT
  AACTCTGGGCCCAAACTAGCTGTGCCTGCGGGTCAGAAGCCAGAGAATGCCAAACCCAACCGAGACCTAA
  AAGTGCAGGCTGGCTCTGACCTGCGGAGGAGACGGCGGGATCTGGCCTCTCATCCAGAGCAGGAGCTGGC
  TCCAAAGGATGGCGTCATCATTAGCTTTAACTCCCTCCCTAATGTTCAGGTGAACGACCTCCGCAGTGCT
  CTGGACACCCAGCTCCGCCAGGCTGCAGGGGCTGCGTTGCAAGTGGTACACAGCCGACAGATTAAACAGT
  TGTCTGGAGATCTGGAGGAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211603 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAASTSKWGLITNVVNSIVGVSVLTMPFCFKQCGIVLGALLLVFCSWMTHQSCMFLVKSASLSKRRTYA
  GLAFHAYGKAGKMLVETSMIGLMLGSCITFYVVIGDLGSNFFAPLLGLQVTRTVRVFLLFAVSLFIVLPL
  SLQRNMMASIQSFSAMALLFYTVFMFVIVLSSLKHGLFSGQWLRQVSYIRWEGVFRCVPIFGMSFACQSQ
  VLPTYDSLDEPSVKTMSSIFASSLNVVTAFYVMVGFFGYVSFTDATTGNVLIHFPSNPVTEMIRVGFVMS
  VAVGFPMMILPCRQALNTLLFEQQQKDGTFAAGGYMPPLRFKVLTLSVVFGTMVGGVMIPNVETILGFTG
  ATMGSLICFICPALIYKKAHKNAPSAQVVLWVGLGILVVSTLTTLSVTEEAPLDLTQEARSGHRGDAEGA
  MKVEAARLSVQDPVVVVAEDSQEKLKPAEDKEVLEQAQIKGPVDVPGGEAPKEKQEAAQLDRPGQGIAVP
  MGEAHRHEPPIPHDKVVVDEGQDQEGPEEKKPPPRLPDEGDPAGRGQGAPPLPESEKEKQEPERGGEGKR
  PGQVLAVGETEHPQKVPEANGQPPVQPRKEDSRPGNRDPQPAAQARDSVELKALAADDGREPAQKAGGAL
  WKPVESAAESDAGGKAGLPVQRPEAAEQREKKEAEQQGGDQAGSKLEEAGRAEMLDHAVLLQVIQEQQVQ
  QKRLLDQQEKLLAVIEEQHKEIRQQRQEGEEDKPKPDVQPEPGVAVLRGQEEEAEHAGETLGDDPSQPLQ
  PVLGAPRGRPAPSQDMGQHLPGEVKVLPGRDLADLPAGGSETEPQGAPIDLREDPKAAIKAAGAGKELVP
  GDLEAVHKAAPPEVPKSPEKQVAKAVAGQRQDVFGEGSEERKETGKEAMAPGADTQKEAVQPLVGAEAKD
  TKSRQSGPTKAPVQTQAKFHPEPQAIFDTGQGSHPEVRSEAPRAVHIPPEEQHKGKGGAAIQEAKQRPDP
  NSGPKLAVPAGQKPENAKPNRDLKVQAGSDLRRRRRDLASHPEQELAPKDGVIISFNSLPNVQVNDLRSA
  LDTQLRQAAGAALQVVHSRQIKQLSGDLEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164800
• ORF Size: 3246 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164800.1, NP_001158272.1
• RefSeq Size: 4565 bp
• RefSeq ORF: 3246 bp
• Locus ID: 72055
• MW: 116.3 kDa