Eif2ak3 (NM_010121) Mouse Tagged ORF Clone

CAT#: MR211654

Eif2ak3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif2ak3
• Synonyms: Pek; Perk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211654 representing NM_010121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGCCACCCGGCCCGGGCCGCGCGCGCTGCTGCTGCTTCTGTTCCTGCTGCTGGGCTGCGCGG
  CGGGGATCTCGGCGGTCGCGCCCGCCCGCAGTTTGCTTGCTCCCGCGTCGGAGACAGTGTTTGGCTTAGG
  GGCAGCGGCCGCCCCGACTTCGGCCGCGCGGGTGCCTGCGGTGGCAACGGCCGAAGTGACCGTGGAGGAC
  GCCGAGGCATTGCCGGCTGCCGCTGGCGAACCGGAGTCACGCGCGACGGAGCCCGATGACGACGTGGAAC
  TGCGGCCTCGCGGCAGGTCCTTGGTAATCATCAGCACTTTAGATGGACGAATCGCTGCACTGGATGCCGA
  GAATGATGGGAAAAAGCAGTGGGATTTGGACGTGGGGTCTGGTTCCTTGGTTTCATCTAGCCTCAGCAAG
  CCAGAGGTGTTTGGGAACAAGATGATCATCCCCTCCCTGGATGGAGACCTCTTCCAGTGGGACCGGGACC
  GAGAGAGCATGGAGGCCGTCCCCTTCACGGTGGAGTCCCTGCTCGAATCTTCCTACAAGTTTGGAGATGA
  TGTTGTTCTGGTTGGAGGGAAATCTCTGACTACATACGGACTCAGTGCTTACAGTGGAAAGCTGAGGTAT
  ATCTGTTCTGCCTTGGGATGTCGCCGATGGGATAGTGATGAAATGGAAGAAGAGGAAGACATCTTGCTTC
  TGCAGCGTACGCAGAAGACTGTGCGAGCTGTCGGGCCTCGAAGCGGCAGTGAGAAGTGGAATTTCAGTGT
  TGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACTAGAGCCGGATTCATTGAAAGCACCTTTAAA
  CCGGGTGGAAACAAAGAAGACTCTAAAATTATTTCAGATGTGGAAGAACAAGAAGCCACCATGCTGGACA
  CAGTGATAAAAGTTTCCGTTGCTGATTGGAAGGTCATGGCGTTTAGTAGGAAGGGAGGCCGCCTGGAATG
  GGAGTACCAGTTTTGTACTCCCATCGCGTCCGCCTGGCTGGTGAGGGATGGCAAGGTCATCCCCATCAGC
  CTGTTTGATGATACAAGTTACACAGCCAGCGAAGAAGCCTTGGGAGACGAAGAAGACATTGTAGAGGCTG
  CTCGGGGAGCCACAGAGAACAGCGTGTACTTAGGGATGTACAGAGGCCAGCTGTACCTGCAGTCGTCCGT
  CAGGGTCTCAGAAAAGTTCCCTACAAGCCCAAAGGCCTTGGAGTCTGTAAATGGCGAAAATGCAATTATT
  CCTCTGCCGACGATCAAATGGAAGCCCTTAATCCATTCTCCTTCTAGGACTCCTGTCTTGGTTGGGTCTG
  ATGAATTTGACAAATGTCTAAGTAATGATAAGTATTCCCACGAAGAATACAGTAATGGTGCACTTTCAAT
  CCTCCAGTATCCATACGATAACGGTTACTATCTGCCATACTACAAGAGAGAAAGGAATAAGCGGAGCACG
  CAGATCACAGTCAGGTTCCTGGACAGCCCCCACTACAGCAAGAACATCCGCAAGAAGGACCCTATCCTCC
  TGCTGCACTGGTGGAAGGAGATATTCGGGACGATCCTGCTTTGCATCGTAGCCACGACCTTCATCGTGCG
  CAGGCTTTTCCATCCTCAGCCCCACAGGCAGCGGAAGGAGTCTGAAACTCAGTGCCAGACTGAAAGTAAA
  TACGACTCCGTGAGTGCCGATGTCAGTGACAACAGCTGGAATGACATGAAGTACTCAGGATACGTATCCC
  GATATCTAACAGATTTTGAGCCAATTCAGTGCATGGGTCGTGGTGGCTTTGGCGTTGTCTTTGAAGCTAA
  AAACAAAGTAGATGACTGCAATTACGCTATCAAGAGGATCCGGCTCCCCAACAGGGAGTTGGCACGGGAG
  AAGGTAATGCGGGAAGTTAAAGCCTTGGCTAAGCTGGAACACCCAGGCATTGTGAGGTATTTCAACGCCT
  GGCTGGAAACCCCACCAGAGAAGTGGCAAGAAGAGATGGATGAGATCTGGCTCAAAGACGAAAGCACAGA
  CTGGCCGCTCAGCTCCCCTAGCCCGATGGATGCCCCATCTGTTAAGATCCGAAGGATGGATCCTTTCTCT
  ACAAAAGAGCAGATCGAAGTCATAGCTCCTTCTCCTGAAAGAAGTCGGTCTTTCTCGGTGGGCATTTCCT
  GTGGCCAGACAAGCTCATCGGAGAGCCAGTTCTCTCCCCTGGAGTTCTCAGGGACAGACTGCGGAGACAA
  CAGTGACTCAGCGGACGCAGCCTACAACCTCCAGGACAGTTGCCTGACGGACTGCGAGGACGTGGAAGAT
  GGCACCGTGGACGGCAATGACGAGGGACACTCCTTTGAACTTTGTCCGTCCGAAGCTTCTCCCTATACCC
  GGTCAAGGGAAGGAACGTCCTCCTCCATAGTGTTTGAGGACTCTGGCTGCGGCAACGCGTCCAGTAAGGA
  GGAGCCCAGAGGGAACCGGCTGCATGATGGCAACCATTATGTTAATAAGCTAACTGATCTCAAGTGCTCC
  AGCAGCAGGTCTTCTTCAGAAGCCACCACCTTGTCTACCTCCCCTACCAGGCCAACCACTCTAAGCTTGG
  ATTTCACCAAGAACACTGTGGGCCAGCTCCAGCCCAGCTCCCCCAAGGTGTATCTGTACATTCAGATGCA
  GCTGTGCAGGAAGGAGAACCTCAAAGACTGGATGAACCGGCGCTGCAGCTTGGAGGACCGGGAGCACGGC
  GTGTGCCTGCACATCTTCCTGCAGATCGCAGAGGCAGTGGAGTTCCTGCACAGCAAGGGACTCATGCACA
  GGGACCTCAAGCCTTCCAACATATTCTTCACAATGGATGATGTGGTCAAGGTTGGGGACTTTGGACTGGT
  GACTGCTATGGACCAAGATGAAGAAGAGCAGACTGTACTGACTCCAATGCCAGCCTATGCTACGCACACG
  GGACAAGTAGGGACCAAGCTATACATGAGCCCAGAGCAGATTCATGGAAACAACTACTCCCATAAAGTGG
  ACATCTTCTCTTTAGGCTTGATTCTGTTTGAACTCCTCTACCCATTCAGCACCCTGATGGAACGAGTCCG
  GATTTTAACTGATGTCAGAAATCTCAAGTTTCCTCTACTGTTCACTCAGAAATATCCCCAAGAGCATATG
  ATGGTTCAAGACATGCTCTCTCCATCCCCCACGGAGCGGCCTGAAGCCACAGACATCATTGAAAATGCCA
  TATTTGAGAACTTGGAGTTTCCCGGGAAAACGGTTCTGAGACAGCGGTCCCGCTCCATGAGTTCATCTGG
  AACAAAACATTCCAGACAGCCCAGCTGCTCGTACAGCCCACTGCCTGGCAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211654 representing NM_010121
  Red=Cloning site Green=Tags(s)
  
  MERATRPGPRALLLLLFLLLGCAAGISAVAPARSLLAPASETVFGLGAAAAPTSAARVPAVATAEVTVED
  AEALPAAAGEPESRATEPDDDVELRPRGRSLVIISTLDGRIAALDAENDGKKQWDLDVGSGSLVSSSLSK
  PEVFGNKMIIPSLDGDLFQWDRDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKLRY
  ICSALGCRRWDSDEMEEEEDILLLQRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIESTFK
  PGGNKEDSKIISDVEEQEATMLDTVIKVSVADWKVMAFSRKGGRLEWEYQFCTPIASAWLVRDGKVIPIS
  LFDDTSYTASEEALGDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRVSEKFPTSPKALESVNGENAII
  PLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKYSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRST
  QITVRFLDSPHYSKNIRKKDPILLLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESETQCQTESK
  YDSVSADVSDNSWNDMKYSGYVSRYLTDFEPIQCMGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELARE
  KVMREVKALAKLEHPGIVRYFNAWLETPPEKWQEEMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFS
  TKEQIEVIAPSPERSRSFSVGISCGQTSSSESQFSPLEFSGTDCGDNSDSADAAYNLQDSCLTDCEDVED
  GTVDGNDEGHSFELCPSEASPYTRSREGTSSSIVFEDSGCGNASSKEEPRGNRLHDGNHYVNKLTDLKCS
  SSRSSSEATTLSTSPTRPTTLSLDFTKNTVGQLQPSSPKVYLYIQMQLCRKENLKDWMNRRCSLEDREHG
  VCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYATHT
  GQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFSTLMERVRILTDVRNLKFPLLFTQKYPQEHM
  MVQDMLSPSPTERPEATDIIENAIFENLEFPGKTVLRQRSRSMSSSGTKHSRQPSCSYSPLPGN
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010121
• ORF Size: 3342 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010121.1, NM_010121.2, NM_010121.3, NP_034251.2
• RefSeq Size: 4512
• RefSeq ORF: 3345
• Locus ID: 13666
• MW: 125.1 kDa
• Gene Summary: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in a similar gene in human are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]