Asap1 (NM_010026) Mouse Tagged ORF Clone

CAT#: MR211708

Asap1 (Myc-DDK-tagged) - Mouse ArfGAP with SH# domain, ankyrin repeat and PH domain1 (Asap1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asap1
• Synonyms: AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211708 representing NM_010026
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATCTTCAGCCTCCCGGCTCTCCAGTTTTTCCTCCAGAGATTCACTATGGAATCGGATGCCGGACC
  AGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC
  CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC
  CTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAAAATGAAGAAA
  ACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTTGGCACCGC
  TTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCAGGGTCTGAGC
  CACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGAGATCTCAAGA
  AGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGAAAAGAGAGCA
  CGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAATGGAAAAGGAA
  CGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACCAAAAAGGGTG
  TGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATGGCTTGAAAAC
  GGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGAT
  GAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGATCCGAAAGAAG
  TAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTTCTAGAAGAGACTCTCAGAGCCGGCAAGGTGGGTA
  CAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGT
  GATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACG
  CAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACAA
  GAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATATA
  GCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCA
  CAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATGA
  CATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTATA
  GAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAAT
  TAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAAA
  TTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGCA
  AAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTG
  AGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCC
  ACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGACA
  TCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAATA
  CAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCAC
  CGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTGT
  GAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAATC
  TTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAACG
  CTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGGG
  TTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACGA
  GCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTCC
  AACTGGCCCACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAG
  AGGCCTCCTCCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCACGGGC
  CCCCAAACAAAGGCGCAATTCCTTGGGGTAATGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACAA
  GTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCT
  AAACTACCTCAGAAAGTGGCACTAAGGAAGACGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAACA
  TCCCACCTGAGACTTTTCAGAAATCATCACAGTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCT
  GCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTA
  CCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAGA
  TGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGC
  CAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTCC
  AGAGATGCCATCCAGAAGCAAGCATCTGAAGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCCG
  TACCACTGCCGAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCCA
  GGCAGATAATGACGACGAACTCACATTTATTGAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCAG
  GAGTGGTGGATCGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACA
  TCCTGTCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211708 representing NM_010026
  Red=Cloning site Green=Tags(s)
  
  MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTA
  LQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLS
  HNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKE
  RRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQD
  EEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKS
  DGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYI
  AWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCI
  ECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITA
  KYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQT
  SLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQC
  EDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPG
  FSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKK
  RPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLP
  KLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGEL
  PPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQS
  RDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQ
  EWWIGHIEGQPERKGVFPVSFVHILSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010026
• ORF Size: 3441 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010026.1, NM_010026.2, NM_010026.3, NP_034156.2
• RefSeq Size: 6281
• RefSeq ORF: 3444
• Locus ID: 13196
• MW: 127.9 kDa