Tsc1 (BC052399) Mouse Tagged ORF Clone

CAT#: MR211709

Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tsc1
• Synonyms: hamartin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211709 representing BC052399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGTTAGCCAACATTGGGGAGCTGCTCTCCATGCTGGACTCCTCCACACTGGGTGTGCGGGATG
  ACGTGACAGCCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTCAACACGTTGGTTGA
  TTATTACCTGGAAACCAATTCTCAGCCGGTATTGCACATCCTGACCACCTTGCAGGAGCCACACGATAAG
  CACCTCTTGGACAAAATTAATGAGTATGTAGGCAAAGCTGCTACCCGTTTATCCATCCTCTCGCTGCTGG
  GGCATGTTGTGAGACTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTATT
  GAAATGTCTCAAGATGGACACTGATGTTGTGGTCCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTC
  CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTCTCGTCAT
  GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTGTACCTGGTCCATCTTCATGCCAGTGTTTATGCCCT
  CTTTCACCGCCTTTATGGGATGTACCCATGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGTATGAAG
  GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG
  TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT
  AGAGTGTGCCAAAATCTCTCTGGACCCTACAGAAGCCTCGTATGAAGATGGCTATTCTGTGTCACACCAG
  CTCTCTGCTTGCTTCCCTTACCGTTCAGCTGATGTCACCACCAGCCCTTATGTGGACACACAGAATAGCT
  ATGGGGGTTCTACTTCCACCCCTTCCTCCAGCTCTCGGCTGATGTTGTTCAGTCCACCTGGGCAGCTACC
  TCAGAGTTTGAGTTCCCCATCAACACGGCTGTTACCTGAGCCGCTGCAAGCTAGTCTCTGGAGCCCATCT
  GCGGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCAGCTGATTTGTCACATCCGTATA
  GTAAAGCCTTTGGTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCTCCAGC
  CCCACCTTGTCCCCAAGATGACTGTGTGCATGGTTCAGCAGCCCAGGCCTCAGCCACAGCCCCCAGGAAG
  GAAGAAAGAGCAGATTCCTCAAGGCCTTACCTTCACAGACAGTCAAACGACCGAGGATTAGAGGATCCAC
  CTGGAAGCAAAGGTTCCGTTACTCTGAGGAATCTACCTGATTTCCTAGGTGATCTGGCTTCTGAGGAAGA
  CAGTATCGAGAAAGATAAGGAAGAAGCTGCAATATCTAAAGAGCTTTCTGAGATCACTACTGCAGAGGCG
  GATCCTGTAGTTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTGGCTCTCAGCGGA
  AGACTCATTCGGCAGCCTCTGGGACTCAGGGCTCCAGCGTGAACCCTGAGCCTTTGCACTCCTCCCTGGA
  CAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGCAGTGCTGATGTC
  AGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCAGCCCTTGCAAAA
  TCCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCTCTTTGAGGTGGC
  CTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAGAAGACTGAGGAGCTGTTGAAGAAAGTGAAAGGAAAC
  CCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGATAGAGCAGGGAGCAG
  GTGCGCACAGCAAGGAGCTGAGCAGGTTGTCCCTGCCCAGCAAGTCTGTTGACTGGACCCACTTTGGAGG
  CTCTCCTCCCTCAGATGAGCTCCGGACCCTCCGAGACCAGTTGCTTTTACTGCACAATCAGCTGCTGTAC
  GAGCGCTTCAAGCGGCAGCAGCATGCACTCAGGAACAGAAGGCTCCTGCGCAAGGTGATCCGAGCAGCGG
  CTCTGGAGGAACACAATGCAGCAATGAAAGATCAGTTGAAGTTACAAGAGAAGGACATACAGATGTGGAA
  GGTGAGTCTGCAGAAAGAACAAGCCCGATACAGTCAGCTTCAGGAACAACGTGACACCATGGTGACCCAA
  CTGCATAGCCAGATCAGACAGCTACAGCATGACCGAGAAGAATTCTACAACCAGAGTCAGGAGTTACAGA
  CAAAGCTGGAGGACTGCAGAAACATGATTGCGGAGCTTCGGGTGGAGCTGAAGAAGGCTAACAACAAGGT
  GTGCCACACTGAGCTGCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAACAGTGAGTCAGTGCAGCAGCAG
  ATGGAGTTCTTGAATAGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGGAGCAGCTGCAGA
  GCAAGCATCCTGACACCACCAAGGAAGTAGAAATGATGAAAACTGCATATCGGAAAGAGCTAGAGAAAAA
  CAGAAGCCACCTTCTCCAGCAGAACCAAAGGTTGGACGCCTCACAGAGGCGAGTTTTGGAACTGGAGTCT
  CTTCTGGCCAAGAAAGACCACCTTCTCCTAGAACAGAAGAAATATCTTGAGGATGTCAAGAGCCAGGCGA
  GTGGACAGCTGCTGGCTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGTGTTGGAACTGGA
  GATCCTAGACTTGTATGGCAGGTTGGAAAAAGATGGCCGCCTACGGAAACTAGAAGAGGACAGAGCAGAG
  GCAGCAGAGGCAGCAGAAGAGAGGCTTGACTGTTGTAGTGATGGATGCACAGATTCCTTGGTAGGACATA
  ATGAAGAGGCTTCTGGTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCGGGCCAGCTGTGG
  AGGTAGAGTCACTGGAGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAACCCCCGAGCCAG
  AGGTTCAGCAGCATCCCCACCACTGTTGGCTCACTTCCCAGTTCCAAAAGCTTCCTGGGCATGAAGGCCC
  GGGAGCTGTTCCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACAGCGTGACCATGAGTAGCAGCAGCCT
  TTCTGAGACCCTGAAGACAGAACTGGGCAAGGACTCGGGCACAGAAAACAAGACTTCCCTGAGTCTAGAT
  GCCCCACACCCATCTTCCCCAAACTCAGACAATGTGGGGCAGCTCCACATCATGGACTACAATGAGACTC
  ATCCTGAACACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211709 representing BC052399
  Red=Cloning site Green=Tags(s)
  
  MAQLANIGELLSMLDSSTLGVRDDVTAIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDK
  HLLDKINEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITML
  PMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMK
  ENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGYSVSHQ
  LSACFPYRSADVTTSPYVDTQNSYGGSTSTPSSSSRLMLFSPPGQLPQSLSSPSTRLLPEPLQASLWSPS
  AVCGMTTPPTSPGNVPADLSHPYSKAFGTTGGKGTPSGTPATSPPPAPPCPQDDCVHGSAAQASATAPRK
  EERADSSRPYLHRQSNDRGLEDPPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEA
  DPVVPRGGFDSPFYRDSLSGSQRKTHSAASGTQGSSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADV
  SPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKVKGN
  PEEDCVPSTSPMEVLDRLIEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDELRTLRDQLLLLHNQLLY
  ERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQEQRDTMVTQ
  LHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANNKVCHTELLLSQVSQKLSNSESVQQQ
  MEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELES
  LLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLRKLEEDRAE
  AAEAAEERLDCCSDGCTDSLVGHNEEASGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPSQ
  RFSSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTMSSSSLSETLKTELGKDSGTENKTSLSLD
  APHPSSPNSDNVGQLHIMDYNETHPEHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052399
• ORF Size: 3444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052399.1
• RefSeq Size: 4829
• RefSeq ORF: 3446
• Locus ID: 64930
• MW: 177 kDa