Egf (BC017681) Mouse Tagged ORF Clone

CAT#: MR211795

Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egf
• Synonyms: AI790464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211795 representing BC017681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATAC
  TCAGCGAGAGAAGCGGGACTTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTC
  TCGGATTGACCCAGATGGAACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGAT
  ATTCATTATAAAAAAGAGAGACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTA
  ACGGGACAGGACTAGAGAAAGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGA
  TGATGAAGTTCTCTGGGTAGACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCC
  CGAGTTCTTCTAAGTTCCTTAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTT
  GGTCTTCAGAGGTGACCGGCAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGA
  GACAGGGGGAATATCGGTGCTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGC
  GAAGGAAGCCACGCTTACATTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAG
  CACGGCACAGTTTGTCTTCAATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGC
  GATTTGGATAGCCAACAAACACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACA
  CCTGGAAAACTGATGGTAGTACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACC
  CCGAACTTCTCAAACAGAGGGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTC
  GAGCGCATGCGCTGAGGGCTACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCC
  ACTCAGAATCACGGCTGTACTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAG
  GATTTGTTCTGCTTCCTGATGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTG
  CAGTCATGGCTGTGTCCTGACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGA
  GATGGGAAGACTTGCACTGGTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCA
  GGCCAGGATCCTGGGAATGTGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGC
  TTCAGGACCACAGCCACTTTTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACA
  GACTACAAAGTTCTGCTCAGCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCA
  AGATATATTTTGCACAGACAGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAG
  ACTGATCACAGAAGGAGTAGATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGG
  ACAGACAGTGGGAAGTCTGTTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGG
  AGAGAATCTCGAGGCCGCGAGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGG
  GATGTCTCCACGGATTGAAAGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTA
  CTGGAACCCAGTGGAATCACGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTG
  TGATTGAAATGGCCAATCTGGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTT
  CTCTCTAGCCGTGTTTGAGGATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAAC
  AAGAGGACTGGCCAAAACAGGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCC
  ATCCATTGGCAAAACCAGGTGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGA
  GAGCCTGGGCACAGCTCGGTGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTC
  CCTCAGGATTATCCAATCCTGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACT
  CCACTCAGGCTGAAGTACCAGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTC
  AGGCATGAACTATGAAGATGACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGA
  GAGACTGCTGAGTGTCAGTGTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGT
  GTGTGCTGGCTAGATCGGACTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTG
  CAGATGCTCAGAAGGCTACGAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCG
  CACAACTGCGCTGAGAATGCCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCC
  CATCCTCGCCCGGACGGAGTTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTT
  GGACCGAAATAGTTATCCAGGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATG
  CATATTGAATCACTGGACAGCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTC
  GAGACCTACGATGGTGGGAGCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGT
  CTGCATGGTGGCACTGGTCCTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAG
  CAGCTATCAAACCCCCCAAAGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACA
  GCAGCAGCGGGGCAGCTGTGGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCC
  CAAGAATGGGAGTCTGCCTGCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTG
  CAGCTCGGGTCAGTGCATCTGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAA
  GCTGCTGGATTCCACCATCAAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTA
  CAGACCTGTGGGGCCGGAGAAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCA
  GACCTGCCACGGCAGACAGAGCCAGTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211795 representing BC017681
  Red=Cloning site Green=Tags(s)
  
  MPWGRRPTWLLLAFLLVFLKISILSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMD
  IHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNS
  RVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSG
  EGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVT
  PGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECA
  TQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGR
  DGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGT
  DYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYW
  TDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNL
  LEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVN
  KRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCL
  PQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDG
  ETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGA
  HNCAENAACTNTEGGYNCTCAGRPSSPGRSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCM
  HIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLLLGMWGTYYYRTRK
  QLSNPPKNPCDEPSGSVSSSGPDSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSL
  QLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAP
  DLPRQTEPVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC017681
• ORF Size: 3600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC017681.1
• RefSeq Size: 4709
• RefSeq ORF: 3602
• Locus ID: 13645
• MW: 172.6 kDa
• Gene Summary: This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]