Egf (BC017681) Mouse Tagged ORF Clone

CAT#: MR211795

  • TrueORF®

Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592)


  "BC017681" in other vectors (4)


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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Egf
Synonyms AI790464
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211795 representing BC017681
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATAC
TCAGCGAGAGAAGCGGGACTTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTC
TCGGATTGACCCAGATGGAACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGAT
ATTCATTATAAAAAAGAGAGACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTA
ACGGGACAGGACTAGAGAAAGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGA
TGATGAAGTTCTCTGGGTAGACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCC
CGAGTTCTTCTAAGTTCCTTAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTT
GGTCTTCAGAGGTGACCGGCAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGA
GACAGGGGGAATATCGGTGCTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGC
GAAGGAAGCCACGCTTACATTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAG
CACGGCACAGTTTGTCTTCAATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGC
GATTTGGATAGCCAACAAACACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACA
CCTGGAAAACTGATGGTAGTACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACC
CCGAACTTCTCAAACAGAGGGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTC
GAGCGCATGCGCTGAGGGCTACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCC
ACTCAGAATCACGGCTGTACTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAG
GATTTGTTCTGCTTCCTGATGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTG
CAGTCATGGCTGTGTCCTGACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGA
GATGGGAAGACTTGCACTGGTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCA
GGCCAGGATCCTGGGAATGTGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGC
TTCAGGACCACAGCCACTTTTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACA
GACTACAAAGTTCTGCTCAGCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCA
AGATATATTTTGCACAGACAGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAG
ACTGATCACAGAAGGAGTAGATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGG
ACAGACAGTGGGAAGTCTGTTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGG
AGAGAATCTCGAGGCCGCGAGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGG
GATGTCTCCACGGATTGAAAGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTA
CTGGAACCCAGTGGAATCACGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTG
TGATTGAAATGGCCAATCTGGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTT
CTCTCTAGCCGTGTTTGAGGATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAAC
AAGAGGACTGGCCAAAACAGGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCC
ATCCATTGGCAAAACCAGGTGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGA
GAGCCTGGGCACAGCTCGGTGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTC
CCTCAGGATTATCCAATCCTGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACT
CCACTCAGGCTGAAGTACCAGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTC
AGGCATGAACTATGAAGATGACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGA
GAGACTGCTGAGTGTCAGTGTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGT
GTGTGCTGGCTAGATCGGACTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTG
CAGATGCTCAGAAGGCTACGAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCG
CACAACTGCGCTGAGAATGCCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCC
CATCCTCGCCCGGACGGAGTTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTT
GGACCGAAATAGTTATCCAGGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATG
CATATTGAATCACTGGACAGCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTC
GAGACCTACGATGGTGGGAGCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGT
CTGCATGGTGGCACTGGTCCTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAG
CAGCTATCAAACCCCCCAAAGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACA
GCAGCAGCGGGGCAGCTGTGGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCC
CAAGAATGGGAGTCTGCCTGCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTG
CAGCTCGGGTCAGTGCATCTGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAA
GCTGCTGGATTCCACCATCAAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTA
CAGACCTGTGGGGCCGGAGAAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCA
GACCTGCCACGGCAGACAGAGCCAGTTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211795 representing BC017681
Red=Cloning site Green=Tags(s)

MPWGRRPTWLLLAFLLVFLKISILSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMD
IHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNS
RVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSG
EGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVT
PGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECA
TQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGR
DGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGT
DYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYW
TDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNL
LEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVN
KRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCL
PQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDG
ETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGA
HNCAENAACTNTEGGYNCTCAGRPSSPGRSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCM
HIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLLLGMWGTYYYRTRK
QLSNPPKNPCDEPSGSVSSSGPDSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSL
QLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAP
DLPRQTEPVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC017681
ORF Size 3600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq BC017681.1
RefSeq Size 4709
RefSeq ORF 3602
Locus ID 13645
MW 172.6 kDa
Gene Summary This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]

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