Col2a1 (BC052326) Mouse Tagged ORF Clone

CAT#: MR211978

Col2a1 (Myc-DDK-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col2a1
• Synonyms: Del1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211978 representing BC052326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCGCGGTCCTACGGTGTC
  AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAGAGATATCAT
  AGGACCCAGAGGACCTCCTGGCCCTCAGGGACCTGCAGGTGAACAAGGACCCAGAGGTGATCGTGGTGAC
  AAGGGAGAAAAGGGTGCGCCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGCC
  CCGCTGGCCCTCCAGGTCCCCCTGGTCCCCCTGGCCTTAGTGCAGGAAACTTCGCGGCTCAGATGGCTGG
  AGGGTATGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGGCCCATGGGCCCCATGGGACCC
  CGTGGACCCCCAGGCCCTGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG
  AGCCTGGTGTCTCTGGTCCCATGGGTCCCCGAGGTCCTCCTGGCCCTGCTGGAAAACCTGGTGACGACGG
  TGAAGCTGGGAAGCCCGGAAAGTCTGGGGAAAGAGGCCTCCCTGGCCCTCAGGGTGCTCGTGGATTCCCA
  GGAACCCCGGGTCTCCCCGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGACGGTGCTAAGGGGGAAG
  CTGGTGCTCCGGGTGTGAAGGGTGAGAGTGGTTCCCCTGGTGAGAACGGATCCCCGGGCCCAATGGGTCC
  CCGTGGCCTGCCTGGTGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGGGGTAACGATGGC
  CAGCCAGGCCCCGCTGGACCTCCGGGTCCTGTGGGTCCCGCAGGTGGTCCTGGCTTCCCTGGTGCTCCTG
  GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCTCGCGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAGCC
  TGGCAATCCTGGGTCCCCTGGGCCTGCAGGTGCTTCTGGTAACCCAGGGACTGATGGTATTCCTGGAGCC
  AAAGGATCCGCTGGTGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC
  CTCAAGGTGCAACTGGTCCCCTTGGCCCCAAAGGTCAGGCGGGTGAACCTGGCATTGCTGGCTTTAAAGG
  TGATCAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAAGGAGCCCCTGGCCCCGCTGGTGAAGAA
  GGCAAACGAGGTGCTCGAGGAGAGCCGGGTGGTGCTGGACCAATCGGACCCCCTGGAGAGAGAGGTGCTC
  CTGGCAACCGTGGATTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC
  CAGTGGCTTGACTGGTCCCAAGGGAGCCAACGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA
  GCCAGGGGTCTTACCGGTCGCCCTGGTGACGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG
  GTGAAGACGGTCGCCCTGGACCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC
  TGGCCCCAAAGGTGCCAACGGCGAGCCTGGCAAAGCTGGTGAGAAGGGTCTGGCTGGCGCTCCTGGTCTG
  AGAGGTCTTCCTGGAAAAGACGGTGAGACGGGAGCCGCAGGACCCCCCGGCCCCAGTGGACCTGCTGGTG
  AACGAGGCGAGCAGGGCGCTCCTGGACCATCAGGGTTCCAGGGACTTCCTGGCCCTCCCGGTCCCCCAGG
  TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCTCCTGGCCTTGTGGGTCCTCGG
  GGCGAGCGAGGTTTCCCAGGTGAACGTGGCTCTCCTGGTGCTCAGGGCCTTCAGGGTCCCCGAGGCCTCC
  CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCAGCTGGCCCAGATGGCCCCCCTGGGGCTCAGGGGCC
  TCCAGGTCTACAGGGAATGCCTGGTGAGAGAGGAGCCGCTGGCATTGCTGGGCCCAAGGGAGACAGAGGC
  GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGGAAGGATGGCGGCCGAGGTCTGACTGGGCCCATCG
  GACCCCCAGGCCCAGCAGGGGCCAACGGCGAGAAGGGAGAAGTCGGACCTCCTGGCCCGTCAGGAAGTAC
  CGGAGCTCGAGGTGCCCCGGGTGAACGCGGAGAGACCGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT
  CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGTGATCAAGGAGAAGCCGGACAGAAAGGAGATGCTGGTG
  CCCCCGGCCCACAAGGCCCCTCGGGAGCCCCTGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG
  AGCCCGAGGTGCCCAAGGTCCCCCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA
  GGTGCTAATGGCAATCCTGGACCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGTTC
  GAGGAGACAGTGGCCCCCCTGGCAGAGCTGGTGACCCCGGTCTTCAAGGTCCTGCAGGAGCTCCTGGCGA
  GAAAGGAGAACCTGGAGATGATGGTCCCTCTGGTCTTGATGGTCCTCCAGGTCCCCAGGGGCTGGCTGGT
  CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCCGGCCTTCCCGGCCCATCGG
  GTGAGCCCGGCAAGCAGGGTGCACCTGGCGCGTCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC
  TGGCCTGACAGGGCCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGAAGA
  GATGGTGCAGCTGGAGTCAAGGGAGATCGTGGTGAGACTGGAGCACTGGGTGCCCCTGGAGCTCCTGGGC
  CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG
  TCCTATGGGTCCCTCAGGACCTGCTGGAGCCCGTGGGATTGCAGGCCCTCAAGGCCCCCGAGGTGACAAA
  GGAGAATCTGGAGAGCAGGGCGAGAGGGGACTGAAGGGACACCGAGGTTTCACTGGACTGCAGGGTCTGC
  CTGGCCCTCCGGGTCCTTCTGGAGATCAGGGTGCTTCTGGCCCTGCTGGTCCTTCTGGCCCTAGAGGTCC
  ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT
  CCCCGTGGACGCTCAGGAGAAACAGGCCCTGTTGGTCCCCCTGGAAGTCCCGGTCCTCCTGGCCCTCCAG
  GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGGCAGAGAGAGAAGGGCCCCGACCC
  CATGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCACGACGTGGAGGTGGACGCTACA
  CTCAAGTCACTGAACAACCAGATTGAGAGCATCCGCAGCCCCGACGGCTCCCGCAAGAACCCTGCTCGCA
  CTTGCCAAGACCTGAAACTCTGCCACCCCGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG
  CTGCACCTTGGACGCCATGAAAGTTTTCTGCAACATGGAGACCGGCGAGACTTGCGTCTACCCCAACCCA
  GCGACTGTCCCTCGGAAAAACTGGTGGAGCAGCAAGAGCAAGGAAAAGAAACACATCTGGTTTGGAGAGA
  CCATGAACGGTGGCTTCCACTTCAGCTATGGCGATGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT
  GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATCGCC
  TACCTGGACGAAGCGGCTGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTAGAGATGA
  GGGCCGAGGGCAACAGCAGGTTCACATACACTGCCCTGAAGGATGGCTGCACGAAACACACTGGTAAGTG
  GGGCAAGACCGTCATCGAGTACCGATCACAGAAGACCTCCCGCCTTCCCATTATTGACATCGCACCCATG
  GACATTGGAGGGGCTGAACAGGAATTTGGTGTGGACATAGGGCCTGTCTGCTTCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211978 representing BC052326
  Red=Cloning site Green=Tags(s)
  
  MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGD
  KGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGP
  RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFP
  GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG
  QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA
  KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEE
  GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPG
  ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL
  RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR
  GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG
  DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP
  PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP
  GANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAG
  QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR
  DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK
  GESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG
  PRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDAT
  LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNP
  ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA
  YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPM
  DIGGAEQEFGVDIGPVCFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052326
• ORF Size: 4257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052326.1
• RefSeq Size: 4856
• RefSeq ORF: 4259
• Locus ID: 12824
• MW: 178 kDa
• Gene Summary: This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]