Bcl9l (BC082304) Mouse Tagged ORF Clone

CAT#: MR211995

Bcl9l (Myc-DDK-tagged) - Mouse B-cell CLL/lymphoma 9-like (cDNA clone MGC:90769 IMAGE:6856460)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcl9l
• Synonyms: MGC7014, DLNB11, BCL9-2, B9L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211995 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCAGAAAATAAACTGACCAATCATGGCAAGACAGGGAATGGAGGGGCCCAATCCCAGCACCAGA
  ATGTGAACCAAGGACCCACCTGCAACCTGGGCTCCAAGGGCGTGGGGGCGGGGAGCCATGGGGCCAAGGC
  CAACCAGATCTCACCTAGCAACTCAAGTCTGAAGAACCCCCAGGCAGGAGTGTCTCCTTTCAGCTCACTC
  AAGGGCAAGGTGAAGCGCGAGAGGAGTGTGTCTGTGGACTCTGGAGAGCAGCGGGAAGCTGGGACTCCAT
  CCCTCGATTCAGAGGCCAAAGAGGTGGCACCCCGGAGTAAACGGAGGTGTGTGCTGGAGCGGAAGCAGCC
  GTACAGTGGGGACGAATGGTGCTCTGGGCCAGACAGCGAGGAGGACGACAAGCCCATTGCGGCCGCCCAC
  AATTGTAATGTAGCAGACCCAGCCATGGTGACCCCACAGTTGGGTCCTGGCCAAACTGCCCAACTGCCCC
  TCAGTGAGAGCAGTGCACCAGGCCCCCAACATGGCCCCCAGCCAGGCCTTCGGCCAGACGTTCCTGGGGG
  TGGGGGTGGGGGCGTCCCAGGAAAGCCTCCGTCACAGTTCGTCTATGTCTTCACCACCCATCTGGCCAAC
  ACAGCGGCAGAGGCAGTGCTGCAGGGCCGGGCAGAGTCCATCCTTGCCTACCACCAGCAGAATGTGCCTC
  GGGCCAAGCTGGATCAGGCCCCTAAAGTGCCACCCACCCCAGAACCACTACCCCTGAATACGCCATCAGC
  AGGTACACCACAGTCCCAGCCACCTCCTTTGCCACCGCCACCCCCAGCCCCTGGCAGTGCCCCTCCTGCT
  CTGCCCCCGGAGGGGCCTCCTGAAGACACCAGTCAGGACCTGGCCCCCAACTCAGTGGGAGCTGCCAGTA
  CAGGTGGTGGGACTGGGGGTACCCACCCTAACACCCCAACGGCTGCCACCGCTAACAACCCTCTGCCTCC
  TGGAGGAGACCCTGGCAGTGCCCCTGGCTCCGCCCTATTGGGGGAGGCCACGCCCACCGGAAATGGGCAG
  AGGAACCTGGTGGGCTCTGAGGGCCTGTCCAAAGAGCAGCTGGAGCACCGGGAGCGCTCCCTCCAGACAC
  TGCGGGACATCGAGAGGCTGCTGCTCCGCAGTGGGGAGACTGAGCCCTTCCTCAAGGGGCCCCCGGGAGG
  AGCTGGTGAGGGAGGCCCACCGGCACAAGCGCCCTCTGCTGCTCAACCGCCTCCCTCCGCCCCTCCTGGG
  GGGCTGAAGAAGTATGAGGAGCCTCTGCAGTCAATGATCTCACAGACACAGAGCCTAGGAGGTCCCCCTC
  TGGAGCATGAAGTGCCGGGGCACCCTCAGGGTGGAGACATGGGACAGCAAATGAACATGATGATGCAGAG
  GCTGGGCCAGGACAGTCTGACGCCCGAGCAGGTGGCCTGGCGCAAACTGCAGGAAGAGTACTACGAGGAG
  AAGCGGCGGAAAGAGGAGCAGATTGGATTGCACGGAGGCCGCCCTCTGCAGGACATGGTGGGAATGGGGG
  GTATGATGGGGAGGGGGCCCCCACCTCCTTACCACAGCAAACCTGGGGATCAGTGGCCACCTGGAATGGG
  TGCACAACTCCGAGGGCCTATGGATGTCCAAGATCCCATGCAGCTCCGACCTGGACCTCCCTTCCCTGGC
  CCCCGTTTCCCAGGCAACCAGATGCAAAGGGTGCCCGGATTTGGAGGTATGCAGAGTATGCCCATGGAAG
  TACCCATGAATGCCATGCAGAGACCTGTAAGGCCAGGCATGGCCTGGAATGAAGACTTGCCCCCTATTGG
  GGGACCCAGCAACTTTGCCCAGAATGCCGTGCCCTACCCAGGTGGGCAGGGGGAGGCAGAGCGATTCATG
  ACCCCTCGTGTCCGGGAGGAGCTGCTGAGGCACCAGTTGCTGGAGAAGCGGTCCATGGGCATGCAGCGTC
  CCCTGGGCATGGCAGGTAGCGGCATGGGACAGAGCATGGAAATGGAACGGATGATACAGGCTCATCGACA
  GATGGACCCTGCCATGTTCCCGGGACAGATGACTGGAGGAGATGGTCTCGCCGGCACACCCATGGGCATA
  GAGTTTGGTGGAGGTCGGGGCCTCCTGAGCCCTCCAATGGGACAGTCTGGGCTGCGGGAGGTAGACCCGC
  CTATGGGGCCAGGCAACCTCAACATGAACATGAATGTGAACATGAACATGAACATGAACCTGAATGTGCA
  GATGACGCCCCAGCAGCAGATGCTGATGTCACAGAAGATGCGGGGCCCTGGAGACATGATGGGTCCTCAG
  GGCCTCAGTCCCGAAGAGATGGCTCGGGTTCGGGCCCAGAACAGTAGTGGCATGATGGGGGGTCCGCAGA
  AGATGCTCATGCCTTCACAGTTTCCCAACCAGGGCCAGCAGGGATTCTCTGGGGGCCAGGGACCTTACCA
  AGCCATGCCCCAGGACATGGGCAACACTCCAGACATGTTCAGCCCTGATCAGAGTTCAGTGCCCATGGGC
  ACTGTGGGCACTGCCCGGCTCAGCCATATGCCTCTGCCCCCTGCCTCCAATCCTCCTGGGTCTGTGCACT
  TGGCCTCCAACAGGGGGCTAGGCAGGCGGCCTTCAGATCTCACCATCAGTATTAATCAGATGGGCTCACC
  GGGCATGGGACATCTGAAGTCACCCACCCTTAGCCAGGTGCACTCCCCCCTGGTCACCTCACCCTCTGCC
  AACCTCAAGTCACCCCAGACTCCCTCCCAGATGGTACCCTTGCCTTCTGCCAACCCACCGGGACCTCTCA
  AGTCACCCCAGGTCCTCAGCTCTTCCCTCGGTGTGCGTTCACCCACTGGCTCACCCAGCAGGCTCAAGTC
  TCCCTCCATGGCGGTGCCTTCTCCAGGCTGGGTCGCCTCTCCCAAGACAGCCATGCCTAGTCCTGGGGTC
  TCCCAGAACAAGCAGCCACCTCTCAGCATAAACTCTTCCTCCACCCTGGGCAACGTGGAACAGGGTGCTC
  TTCCACCTAGCGCACCCCGGAACAGCTCCTCCGCTCCTCCCGCCAACCCTTCCAGTGGCCTCATGAACCC
  CAGCCTACCGTTCACATCCTCCCCAGACCCCACCCCTTCCCAGAACCCTCTGTCACTGATGATGTCTCAG
  ATGTCCAAGTACGCCATGCCCAGCTCGACCCCGCTATACCACAACGCCATCAAGACCATCGCCACCTCAG
  ATGACGAGCTGCTGCCTGACCGGCCCCTGCTACCCCCACCCCCACCACCGCAGGGCTCTGGGCCAGGTAT
  CAGCAATAACCAGCCCAACCAGATGCACATGAACCCTGCTGCTGCCCAGAGCCCCATGGGCATGAACTTG
  CCAGGCCAGCAGCCCCTGTCCCATGAGCCTCCCCCTACTATGTTGCCCTCCCCCACCCCTCTGGGGTCCA
  ACATTCCACTGCATCCCAATGCACAGGGGACTGGGGGCTCTTCTCAAAACTCAATGATGATGGCCCCAGG
  AGGCCCAGACTCCCTAAATGCCCCTTGTGGCCCTGTGCCCAGCTCCTCCCAGATGATGTCCTTCCCTCCT
  CGGCTGCAGCAACCTCACGGTGCCATGGCCCCCACCGGGGCCGGGGGCCCAGGCCTGCAGCAGCACTACC
  CTTCAGGCATGGCCCTGCCCCCCGAGGACCTGCCCACCCAGCCACCGGGTCCCATACCCCCCCAGCAGCA
  CCTAATGGGCAAAGGCATGACTGGCCGCATGGGCGACGCATACCCACCCGGGGTGCTCCCTGGGGTGGCA
  TCGGTACTGAATGACCCAGAGCTGAGTGAGGTGATCCGGCCCACCCCTACCGGCATTCCTGAGTTCGACT
  TATCCAGGATCATCCCCTCTGAGAAACCAAGCAGCACCCTCCAGTACTTCCCCAAGAGCGAGAACCAGCC
  CCCCAAGGCCCAGCCCCCCAATCTGCATCTCATGAACCTGCAGAACATGATGGCAGAGCAGACCCCGTCT
  CGACCCCCCAACCTCCCGGGCCAACAGGGGGTCCAGCGGGGGCTCAGCATGTCCATGTGCCACCCTGGAC
  AGATGTCCTTGCTGGGCAGGACAGGTGTGCCCCCACAACAGGGCATGGTGCCCCATGGCCTGCACCAGGG
  GGTCATGTCCCCTCCACAAGGCCTCATGACCCAGCAGAATTTTATGCTGATGAAGCAGAGGGGTGTGGGG
  GGCGAGGTCTACACCCAGCCTCCCCACATGCTCTCCCCACAGGGCTCCCTCATGGGCCCCCCACCCCAGC
  AGAACCTCATGGTGTCCCACCCTCTGCGTCAGCGCAGTGTGTCTCTGGACAGCCAGATGGGCTACCTGCC
  AACGCCGGGCAGCATGGCCAATCTACCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211995 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPENKLTNHGKTGNGGAQSQHQNVNQGPTCNLGSKGVGAGSHGAKANQISPSNSSLKNPQAGVSPFSSL
  KGKVKRERSVSVDSGEQREAGTPSLDSEAKEVAPRSKRRCVLERKQPYSGDEWCSGPDSEEDDKPIAAAH
  NCNVADPAMVTPQLGPGQTAQLPLSESSAPGPQHGPQPGLRPDVPGGGGGGVPGKPPSQFVYVFTTHLAN
  TAAEAVLQGRAESILAYHQQNVPRAKLDQAPKVPPTPEPLPLNTPSAGTPQSQPPPLPPPPPAPGSAPPA
  LPPEGPPEDTSQDLAPNSVGAASTGGGTGGTHPNTPTAATANNPLPPGGDPGSAPGSALLGEATPTGNGQ
  RNLVGSEGLSKEQLEHRERSLQTLRDIERLLLRSGETEPFLKGPPGGAGEGGPPAQAPSAAQPPPSAPPG
  GLKKYEEPLQSMISQTQSLGGPPLEHEVPGHPQGGDMGQQMNMMMQRLGQDSLTPEQVAWRKLQEEYYEE
  KRRKEEQIGLHGGRPLQDMVGMGGMMGRGPPPPYHSKPGDQWPPGMGAQLRGPMDVQDPMQLRPGPPFPG
  PRFPGNQMQRVPGFGGMQSMPMEVPMNAMQRPVRPGMAWNEDLPPIGGPSNFAQNAVPYPGGQGEAERFM
  TPRVREELLRHQLLEKRSMGMQRPLGMAGSGMGQSMEMERMIQAHRQMDPAMFPGQMTGGDGLAGTPMGI
  EFGGGRGLLSPPMGQSGLREVDPPMGPGNLNMNMNVNMNMNMNLNVQMTPQQQMLMSQKMRGPGDMMGPQ
  GLSPEEMARVRAQNSSGMMGGPQKMLMPSQFPNQGQQGFSGGQGPYQAMPQDMGNTPDMFSPDQSSVPMG
  TVGTARLSHMPLPPASNPPGSVHLASNRGLGRRPSDLTISINQMGSPGMGHLKSPTLSQVHSPLVTSPSA
  NLKSPQTPSQMVPLPSANPPGPLKSPQVLSSSLGVRSPTGSPSRLKSPSMAVPSPGWVASPKTAMPSPGV
  SQNKQPPLSINSSSTLGNVEQGALPPSAPRNSSSAPPANPSSGLMNPSLPFTSSPDPTPSQNPLSLMMSQ
  MSKYAMPSSTPLYHNAIKTIATSDDELLPDRPLLPPPPPPQGSGPGISNNQPNQMHMNPAAAQSPMGMNL
  PGQQPLSHEPPPTMLPSPTPLGSNIPLHPNAQGTGGSSQNSMMMAPGGPDSLNAPCGPVPSSSQMMSFPP
  RLQQPHGAMAPTGAGGPGLQQHYPSGMALPPEDLPTQPPGPIPPQQHLMGKGMTGRMGDAYPPGVLPGVA
  SVLNDPELSEVIRPTPTGIPEFDLSRIIPSEKPSSTLQYFPKSENQPPKAQPPNLHLMNLQNMMAEQTPS
  RPPNLPGQQGVQRGLSMSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVG
  GEVYTQPPHMLSPQGSLMGPPPQQNLMVSHPLRQRSVSLDSQMGYLPTPGSMANLPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082304
• ORF Size: 4371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082304, AAH82304
• RefSeq Size: 5472 bp
• RefSeq ORF: 4373 bp
• Locus ID: 80288
• MW: 152.6 kDa