Cltc (NM_001003908) Mouse Tagged ORF Clone

CAT#: MR212064

Cltc (Myc-DDK-tagged) - Mouse clathrin, heavy polypeptide (Hc) (Cltc)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cltc
• Synonyms: 3110065L21Rik; CHC; R74732
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212064 representing NM_001003908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGATTCTGCCCATTCGCTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAA
  ACATTGGCTTCAGTACCTTGACTATGGAGTCAGACAAATTCATCTGCATTAGAGAAAAAGTGGGAGAGCA
  GGCCCAGGTGGTAATCATTGATATGAATGATCCAAGTAATCCAATTCGAAGACCAATCTCAGCAGACAGT
  GCCATCATGAATCCTGCCAGCAAAGTGATTGCACTGAAAGCTGGCAAAACTCTTCAGATATTTAACATTG
  AAATGAAAAGTAAAATGAAGGCCCATACCATGACTGATGATGTCACCTTCTGGAAATGGATCTCTTTGAA
  TACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGTATGGAAGGAGAGTCTCAGCCAGTGAAA
  ATGTTTGATCGTCATTCTAGCCTCGCAGGATGCCAGATCATCAATTACCGTACAGATGCAAAGCAGAAAT
  GGTTGCTTCTCACTGGCATATCTGCACAGCAAAATCGTGTGGTTGGAGCTATGCAGCTGTATTCTGTAGA
  TAGGAAAGTTTCTCAGCCTATTGAAGGGCATGCAGCTAGTTTTGCACAATTTAAGATGGAAGGAAATGCA
  GAAGAATCAACATTATTTTGTTTTGCTGTGCGGGGTCAGGCAGGAGGAAAGTTACATATCATTGAAGTTG
  GTACCCCACCCACAGGGAATCAACCCTTCCCAAAGAAGGCAGTGGATGTTTTCTTTCCTCCAGAAGCACA
  AAATGACTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGATGTGGTGTTCTTGATTACCAAGTATGGT
  TATATTCACCTCTATGATCTTGAGACTGGCACGTGCATCTACATGAACAGAATCAGCGGAGAAACCATCT
  TCGTCACTGCACCTCATGAAGCCACAGCTGGAATAATAGGAGTCAACAGAAAGGGACAGGTCCTGTCAGT
  TTGTGTGGAAGAGGAGAACATAATTCCTTATATCACCAATGTCCTACAAAATCCTGACTTGGCTCTGAGA
  ATGGCTGTGCGGAACAACTTAGCTGGTGCTGAAGAACTCTTTGCCCGGAAATTTAATGCTCTCTTCGCCC
  AGGGAAACTACTCAGAGGCTGCAAAGGTGGCTGCTAATGCTCCAAAGGGAATCCTTCGTACTCCAGATAC
  CATCCGTCGATTCCAGAGTGTCCCAGCTCAGCCAGGTCAAACGTCACCTCTACTTCAGTATTTCGGAATC
  CTTTTGGACCAGGGCCAGCTGAACAAATACGAGTCCTTAGAGTTATGTAGACCTGTACTTCAGCAAGGGC
  GGAAACAGCTTTTGGAGAAATGGCTGAAAGAAGATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTCGT
  TAAATCTGTGGACCCAACATTAGCACTGAGTGTTTACCTCAGGGCCAATGTACCTAATAAAGTCATCCAG
  TGCTTTGCAGAGACAGGCCAAGTCCAGAAGATTGTTTTATATGCTAAAAAAGTTGGCTATACTCCAGACT
  GGATCTTTCTCCTGAGAAATGTCATGCGGATCAGTCCAGATCAAGGACAGCAGTTTGCTCAAATGTTAGT
  TCAGGATGAAGAGCCTCTTGCTGATATCACCCAGATTGTGGATGTCTTTATGGAATACAATCTAATCCAA
  CAATGTACTGCCTTCTTACTTGATGCTTTGAAGAATAATCGTCCCTCTGAAGGTCCTTTGCAGACGCGAT
  TGCTTGAGATGAACCTTATGCACGCACCTCAAGTTGCAGATGCCATTCTCGGGAATCAGATGTTCACCCA
  CTATGACCGGGCTCATATCGCCCAGCTGTGTGAGAAGGCGGGCCTGCTGCAGCGTGCATTAGAACACTTC
  ACCGATTTATATGACATAAAACGTGCAGTTGTTCATACCCATCTTCTTAACCCTGAGTGGTTAGTGAATT
  ACTTTGGGTCCTTATCAGTTGAAGATTCCCTAGAATGCCTCAGAGCCATGCTGTCAGCTAATATTCGTCA
  AAATCTACAGATCTGTGTTCAGGTGGCTTCTAAATATCACGAACAGCTGTCAACTCAGTCTCTGATTGAG
  CTCTTTGAATCATTCAAGAGTTTTGAAGGTCTCTTTTATTTTCTGGGGTCCATTGTTAACTTTAGTCAGG
  ACCCAGATGTGCACTTTAAATATATTCAGGCGGCTTGCAAGACTGGGCAAATCAAAGAAGTGGAAAGAAT
  CTGCAGAGAAAGCAACTGCTATGATCCCGAGCGAGTCAAGAATTTTCTCAAGGAGGCAAAACTAACAGAT
  CAGTTGCCACTTATCATTGTCTGTGATCGGTTTGACTTTGTCCATGACCTGGTGCTGTATTTATATAGAA
  ACAATCTTCAGAAGTATATAGAGATATATGTCCAGAAGGTAAATCCAAGTCGACTTCCTGTGGTTATTGG
  AGGATTGCTGGATGTTGACTGCTCTGAAGATGTCATTAAAAATTTGATTCTTGTTGTGAGAGGTCAATTC
  TCTACTGATGAGCTTGTTGCTGAAGTCGAGAAAAGAAACAGATTGAAGCTGCTTCTTCCTTGGCTTGAGG
  CCAGAATTCATGAGGGCTGTGAGGAGCCTGCTACTCACAATGCACTTGCCAAAATATACATTGACAGTAA
  CAACAACCCAGAGAGATTTCTTCGTGAAAACCCTTATTATGACAGTCGTGTTGTTGGGAAGTACTGTGAG
  AAGAGAGACCCCCATCTGGCTTGTGTTGCTTATGAACGTGGCCAGTGTGACCTGGAGCTCATCAATGTTT
  GCAATGAGAATTCCCTCTTCAAAAGCCTTTCTCGCTACTTAGTCCGTCGAAAGGATCCAGAATTGTGGGG
  TAGTGTGCTACTGGAAAGCAATCCATACAGAAGACCGTTAATTGACCAGGTTGTACAGACAGCCTTGTCT
  GAAACTCAGGACCCAGAAGAAGTGTCAGTAACTGTGAAGGCTTTTATGACAGCAGATCTTCCTAATGAGC
  TCATTGAACTGCTGGAGAAAATCGTTCTTGATAACTCCGTATTCAGTGAGCACAGGAATCTGCAAAATCT
  CCTCATCCTCACTGCAATTAAGGCTGACCGAACACGTGTTATGGAGTATATTAACCGCCTGGATAATTAT
  GATGCTCCAGATATTGCCAACATTGCCATCAGCAATGAGCTGTTTGAAGAGGCATTTGCTATTTTCCGGA
  AGTTTGATGTCAATACTTCAGCAGTGCAGGTGTTGATTGAACATATTGGAAACCTGGATCGTGCATATGA
  GTTTGCTGAACGCTGCAATGAGCCTGCTGTTTGGAGTCAACTTGCAAAAGCTCAGTTGCAGAAAGGAATG
  GTGAAGGAAGCCATTGATTCTTACATCAAAGCAGATGATCCTTCATCTTACATGGAAGTTGTCCAGGCTG
  CCAATGCCAGCGGAAACTGGGAAGAATTGGTGAAGTATTTGCAGATGGCACGCAAGAAGGCTCGAGAGTC
  CTATGTGGAGACAGAATTGATATTTGCACTGGCTAAAACAAACCGCCTTGCAGAATTAGAAGAGTTCATC
  AATGGGCCAAATAATGCTCATATTCAGCAAGTTGGTGACCGTTGTTATGATGAAAAAATGTATGATGCTG
  CTAAGTTGTTGTACAATAATGTTTCTAACTTTGGACGCTTGGCATCTACCCTTGTTCACCTTGGCGAGTA
  TCAGGCAGCAGTTGATGGTGCCAGAAAAGCTAATAGTACTCGAACATGGAAAGAGGTCTGCTTTGCCTGT
  GTAGATGGGAAAGAGTTCCGCCTTGCTCAGATGTGTGGACTCCATATTGTGGTGCATGCAGATGAATTAG
  AGGAGCTTATCAACTACTATCAGGATCGTGGATATTTTGAAGAACTCATCACCATGTTGGAGGCAGCCTT
  GGGACTGGAGCGAGCTCACATGGGAATGTTCACAGAGCTAGCTATTTTGTATTCTAAATTCAAGCCACAG
  AAAATGAGAGAACACCTGGAGTTGTTCTGGTCGAGAGTGAATATTCCTAAGGTGCTGAGAGCTGCAGAAC
  AGGCTCATCTCTGGGCAGAGCTGGTGTTTCTGTATGACAAGTACGAAGAGTATGACAATGCCATCATTAC
  CATGATGAATCACCCCACCGATGCATGGAAGGAAGGGCAGTTCAAAGACATCATCACCAAGGTGGCTAAT
  GTGGAACTGTACTACAAAGCAATCCAGTTCTACTTAGAATTCAAGCCTTTGTTGTTAAATGACTTGCTCA
  TGGTGCTGTCTCCACGGTTGGATCACACTCGTGCAGTCAATTATTTCAGCAAGGTAAAACAGCTACCACT
  GGTGAAACCATATTTACGCTCAGTGCAGAACCACAACAACAAATCTGTGAATGAATCACTGAACAACCTC
  TTTATTACTGAAGAAGATTACCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAACATCT
  CACTTGCTCAGCGTTTGGAAAAACACGAACTCATTGAATTCAGAAGAATTGCTGCTTATCTCTTCAAAGG
  CAACAATCGCTGGAAACAGAGTGTAGAGTTGTGCAAGAAAGACAGCCTTTACAAGGATGCAATGCAGTAT
  GCCTCTGAATCTAAAGACACAGAATTGGCTGAAGAGCTCCTACAGTGGTTTTTGCAGGAAGAAAAAAGAG
  AATGCTTTGGAGCTTGTCTCTTTACCTGTTATGATCTCTTAAGGCCAGATGTCGTCCTGGAAACCGCATG
  GAGACATAATATCATGGATTTCGCCATGCCCTATTTCATCCAGGTCATGAAGGAATATTTGACCAAGGTG
  GATAAATTAGATGCTTCGGAGTCGCTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAGCCCATTGTTT
  ATGGTCAGCCCCAGCTGATGCTAACAGCAGGACCCAGTGTGGCAGTCCCTCCCCAGGCACCTTTTGGTTA
  TGGTTATACTGCACCACCGTATGGGCAGCCACAGCCTGGCTTTGGGTACAGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212064 representing NM_001003908
  Red=Cloning site Green=Tags(s)
  
  MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADS
  AIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVK
  MFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNA
  EESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYG
  YIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALR
  MAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGI
  LLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQ
  CFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQ
  QCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHF
  TDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIE
  LFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTD
  QLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQF
  STDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCE
  KRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALS
  ETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNY
  DAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGM
  VKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFI
  NGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFAC
  VDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQ
  KMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVAN
  VELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNL
  FITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQY
  ASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKV
  DKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003908
• ORF Size: 5025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003908.1, NP_001003908.1
• RefSeq Size: 6178 bp
• RefSeq ORF: 5028 bp
• Locus ID: 67300
• Cytogenetics: 11 C
• MW: 192 kDa