Brca1 (NM_009764) Mouse Tagged ORF Clone

CAT#: MR212086

Brca1 (Myc-DDK-tagged) - Mouse breast cancer 1 (Brca1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212086 representing NM_009764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTATCTGCCGTCCAAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTAGAGT
  GTCCGATCTGTTTGGAACTGATCAAAGAACCTGTTTCCACAAAGTGTGACCACATATTTTGCAAATTTTG
  TATGCTGAAACTTCTTAACCAGAAGAAAGGGCCTTCACAATGTCCTTTGTGTAAGAATGAGATAACCAAA
  AGGAGCCTACAGGGAAGCACAAGGTTTAGTCAGCTTGCTGAAGAGCTGCTGAGAATAATGGCTGCTTTTG
  AGCTTGACACGGGAATGCAGCTTACAAATGGTTTTAGTTTTTCAAAAAAGAGAAATAATTCTTGTGAGCG
  TTTGAATGAGGAGGCGTCGATCATCCAGAGCGTGGGCTACCGGAACCGTGTCAGAAGGCTTCCCCAGGTC
  GAACCTGGAAATGCCACCTTGAAGGACAGCCTAGGTGTCCAGCTGTCTAACCTTGGAATCGTGAGATCAG
  TGAAGAAAAACAGGCAGACCCAACCTCGAAAGAAATCTGTCTACATTGAACTAGACTCTGATTCTTCTGA
  AGAGACAGTAACTAAGCCAGGTGATTGCAGTGTGAGAGACCAGGAATTGTTACAGACCGCCCCTCAAGAA
  GCTGGAGATGAAGGCAAGCTGCACTCTGCAGAAGAGGCTGCTTGTGAGTTTTCTGAGGGCATAAGAAACA
  TTGAACATCATCAATGCAGTGATGATTTAAACCCTACTGAGAATCATGCAACTGAAAGGCATCCAGAAAA
  ATGTCAGAGTATTTCTATTTCAAATGTGTGTGTGGAGCCATGTGGCACAGATGCTCATGCCAGCTCATTA
  CAGCCTGAGACCAGCAGTTTATTGCTCATTGAAGACAGAATGAATGCAGAAAAGGCTGAATTCTGTAATA
  AAAGCAAACAGCCTGGCATAGCAGTGAGCCAGCAGAGCAGATGGGCTGCAAGTAAAGGAACATGTAACGA
  CAGGCAGGTTCCCAGCACTGGGGAAAAGGTAGGTCCAAACGCTGACTCCCTTAGTGATAGAGAGAAGTGG
  ACTCACCCGCAAAGTCTGTGCCCTGAGAATTCTGGAGCTACCACCGATGTTCCTTGGATAACACTAAATA
  GCAGCGTTCAGAAAGTTAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAGCGCATCTGC
  CAGGAGGCACGAGTCAAATGCTGAAGCAGCTGTTGTGTTGGAAGTTTCAAACGAAGTGGATGGGGGTTTT
  AGTTCTTCAAGGAAAACAGACTTAGTAACCCCCGACCCCCATCATACTTTAATGTGTAAAAGTGGAAGAG
  ACTTCTCCAAACCAGTAGAGGATAATATCAGTGATAAAATATTTGGGAAATCCTATCAGAGAAAGGGAAG
  CCGCCCTCACCTGAACCATGTGACTGAAATTATAGGCACATTTATTACAGAACCACAGATAACACAAGAG
  CAGCCCTTCACAAATAAATTAAAACGTAAGAGAAGTACATCCCTTCAACCTGAGGACTTCATCAAGAAAG
  CAGATTCAGCAGGTGTTCAAAGGACTCCTGACAACATAAATCAGGGAACTGACCTAATGGAGCCAAATGA
  GCAAGCAGTGAGTACTACCAGTAACTGTCAGGAGAACAAAATAGCAGGTAGTAATCTCCAGAAAGAGAAA
  AGCGCTCATCCAACTGAATCATTGAGAAAGGAACCTGCTTCCACAGCAGGAGCCAAATCTATAAGCAACA
  GTGTAAGTGATTTGGAGGTAGAATTAAACGTCCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAGGAG
  GAAGTCTTCTATCAGGTGTGCTCTTCCACTTGAACCAATCAGTAGAAATCCAAGCCCACCTACTTGTGCT
  GAGCTTCAAATCGATAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACCATTCCAACCAACAGCCAGCCG
  GGCACCTTAGAGAGCCTCAACTCATCGAAGACACTGAACCTGCAGCGGATGCCAAGAAGAACGAGCCAAA
  TGAACACATAAGGAAGAGACGTGCCAGCGATGCTTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTA
  TTAACTAGCTGTTCAAGTCCTAGAAAATCTCAAGGGCCTGTCAATCCCAGCCCTCAGAGAACAGGAACAG
  AGCAACTTGAAACACGCCAAATGTCTGACAGTGCCAAAGAACTCGGGGATCGGGTCCTAGGAGGAGAGCC
  CAGTGGCAAAACCACTGACCGATCTGAGGAGAGCACCAGCGTATCCTTGGTATCTGACACTGACTACGAC
  ACTCAGAACAGTGTCTCAGTCCTGGACGCTCACACTGTCAGATATGCAAGAACAGGATCCGCTCAGTGTA
  TGACTCAGTTTGTAGCAAGCGAAAACCCCAAGGAACTCGTCCATGGCTCTAACAATGCTGGGAGTGGCAC
  AGAGGGTCTCAAGCCCCCCTTGAGACACGCGCTTAACCTCAGTCAGGAGAAAGTAGAAATGGAAGACAGT
  GAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAA
  AACCTAGAAGTCCCCAAAAGGACTGTGCTCACTCTGTGCCCTCAAAGGAACTGAGTCCAAAGGTGACAGC
  TAAAGGTAAACAAAAAGAACGTCAGGGACAGGAAGAATTTGAAATCAGTCACGTACAAGCAGTTGCGGCC
  ACAGTGGGCTTACCTGTGCCCTGTCAAGAAGGTAAGCTAGCTGCTGATACAATGTGTGATAGAGGTTGTA
  GGCTTTGTCCATCATCTCATTACAGAAGCGGGGAGAATGGACTCAGCGCCACAGGTAAATCAGGAATTTC
  ACAAAACTCACATTTTAAACAATCAGTTTCTCCCATCAGGTCATCTATAAAAACTGACAATAGGAAACCT
  CTGACAGAGGGACGATTTGAGAGACATACATCATCAACTGAGATGGCGGTGGGAAATGAGAACATTCTTC
  AGAGTACAGTGCACACAGTTAGCCTGAATAACAGAGGAAATGCTTGTCAAGAAGCCGGCTCGGGCAGTAT
  TCATGAAGTATGTTCCACTGGTGACTCCTTCCCAGGACAACTAGGTAGAAACAGAGGGCCTAAGGTGAAC
  ACTGTGCCTCCATTAGATAGTATGCAGCCTGGTGTCTGTCAGCAAAGTGTTCCTGTAAGTGATAAGTATC
  TTGAAATAAAAAAGCAGGAGGGTGAGGCTGTCTGTGCAGACTTCTCTCCATGTCTATTCTCAGACCATCT
  TGAGCAATCTATGAGTGGTAAGGTTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGCTGGATGATGTT
  GAAATACAGGGACATACTAGCTTTGGTGAAGGTGACATAATGGAGAGATCTGCTGTCTTTAACGGAAGCA
  TCCTGAGAAGGGAGTCCAGTAGGAGCCCTAGTCCTGTAACCCATGCATCGAAGTCTCAGAGTCTCCACAG
  AGCGTCTAGGAAATTAGAATCGTCAGAAGAGAGCGACTCCACTGAGGATGAAGATCTTCCCTGCTTCCAA
  CACTTACTGAGCAGAATAAGCAACACACCTGAGCTTACCAGATGCAGCAGTGCTGTGACACAGCGTATGC
  CAGAGAAAGCGGAGGGGACCCAAGCACCATGGAAGGGTAGCAGCAGTGACTGCAATAATGAGGTGATCAT
  GATAGAGGCATCTCAGGAGCATCAGTTTAGTGAGGATCCAAGATGCTCTGGCAGCATGTTCTCTTCACAG
  CACAGTGCTGCCCAAGGGTCAACTGCAAATGCAAACTCCCAGGATTCCAATTTTATTCCACCTTCCAAAC
  AGAGGAGTCACCAGTGTGGGAATGAGGAAGCTTTCCTAAGTGACAAGGAATTGATTTCAGATAACGAGGA
  AATGGCAACTTGCCTAGAAGAGGATAATGACCAAGAAGAGGATAGTATAATCCCAGATTCAGAGGCATCC
  GGATACGAGAGTGAAACAAACCTTTCTGAAGACTGCTCGCAGAGTGATATTTTAACCACTCAGCAGCGGG
  CGACCATGAAGTATAACCTGATAAAGCTGCAGCAGGAAATGGCTCACCTGGAAGCTGTGCTGGAGCAGCG
  TGGGAACCAGCCTTCTGGCCACTCCCCTTCCCTCCTAGCGGACCCTTGTGCCCTGGAAGACCTGCCAGAT
  CTGGAACCAAACATGTCAGGAGCAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCCAAA
  ATTTGAAGAGCGCTTGTGATGACAAATTCCAACTACAACATCTGGAGGGTCCCACCAGTGGAGATGACGA
  GTCAGGGATGGGAAGGCCTTCCCCTTTTAAATCTCCGTTGGCAGGCAGTAGGGGCTCTGCACATGGCTGC
  TCTAGGCATCTTCAAAAGAGAAACTCCCCCTCTCAGGAGGAGCTCCTCCAGCCTGCTGGATCAGAGGCGT
  CATCTGAGCCACACAATTCAACAGGGCAGTCTTGCCTGCCAAGGCGAGAGCTAGAAGGAACCCCATACCT
  GGGATCTGGAATCAGCCTTTTCTCTAGTAGAGACCCCGAATCTGAGTCCCCTAAAGAGCCAGCCCACATT
  GGCACCACACCAGCTTCAACCTCTGCACTGAAAATACCCCAAGGTCAAGTTGCTTTCCGGAGTGCAGCTG
  CTGCTGGTGCTGATAAAGCAGTGGTAGGAATTGTGAGCAAGATAAAGCCGGAATTGACATCTTCAGAAGA
  AAGAGCGGATAGAGACATATCCATGGTGGTGTCAGGCTTGACCCCCAAAGAAGTAATGACCGTGCAAAAG
  TTTGCTGAAAAATACCGCCTCACTTTAACTGACGCAATTACTGAGGAGACTACACATGTAATTATAAAAA
  CAGATGCGGAGTTTGTGTGTGAGCGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAAGTGGATAGT
  TAGCTATTCATGGGTGGTCCGGTCTATCCAAGAAAGAAGACTTCTGAATGTGCATGAATTTGAAGTCAAA
  GGAGATGTTGTGACTGGAAGAAATCACCAAGGTCCAAGGCGATCCAGAGAATCCCGGGAAAAGCTCTTCA
  AGGGCCTACAGGTCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAGGATGCTGCA
  GCTGTGTGGGGCTTCCGTGGTGAAGGAGCTTCCATCGCTCACCCATGACACAGGTGCTCATCTAGTTGTG
  ATCGTGCAGCCAAGCGCCTGGACAGAAGACAGCAACTGCCCAGATATTGGGCAGCTGTGCAAGGCACGTC
  TTGTGATGTGGGACTGGGTGTTGGACAGTCTATCCAGCTACCGGTGTCGGGATCTGGATGCCTACCTGGT
  ACAGAATATCACCTGTGACAGTAGTGAGCCACAAGACTCCAATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212086 representing NM_009764
  Red=Cloning site Green=Tags(s)
  
  MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITK
  RSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQV
  EPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQE
  AGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSL
  QPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKW
  THPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGF
  SSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQE
  QPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEK
  SAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCA
  ELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGL
  LTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYD
  TQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDS
  ELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAA
  TVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKP
  LTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVN
  TVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDV
  EIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQ
  HLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQ
  HSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEAS
  GYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPD
  LEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGC
  SRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHI
  GTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQK
  FAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVK
  GDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVV
  IVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009764
• ORF Size: 5436 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009764.2, NM_009764.3, NP_033894.3
• RefSeq Size: 6648
• RefSeq ORF: 5439
• Locus ID: 12189
• MW: 198.8 kDa