Myh1 (NM_030679) Mouse Tagged ORF Clone

CAT#: MR212099

Myh1 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myh1
• Synonyms: A530084A17Rik; IId; IId/x; MdMs; MHC-2X/D; MHC2X/D; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f2; Myhsf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212099 representing NM_030679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC
  GAATCGAGGCTCAGAACAAGCCTTTTGACGCCAAGTCATCGGTGTTTGTGGTGGATGCTAAGGAGTCTTT
  TGTAAAAGCAACGGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGGCGGAACTACTGTA
  ACAGTAAAAGATGATCAGGTCTACCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA
  TGACCCACCTGCATGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC
  CTACTCGGGCCTGTTCTGCGTCACTGTCAACCCCTACAAGTGGCTGCCAGTGTATAACGCAGAGGTGGTG
  GCAGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTACC
  AGTTCATGCTGACAGATCGGGAGAACCAGTCTATTTTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT
  GAACACGAAACGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAAAAGAAGGAGGAGGCA
  ACTTCTGGCAAAATGCAGGGGACGCTGGAGGACCAAATCATCAGCGCCAACCCCCTGCTGGAGGCCTTTG
  GGAACGCCAAGACCGTGAGGAATGACAACTCTTCCCGCTTTGGTAAGTTCATCAGGATCCACTTTGGTAC
  CACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTCACTTTCCAGCTA
  AAAGCTGAGAGAAGCTACCACATTTTTTACCAGATTATGTCTAACAAGAAGCCGGATCTAATCGAAATGC
  TCCTGATCACCACCAACCCATACGACTACGCCTTTGTCAGTCAGGGGGAGATCACGGTGCCCAGCATTGA
  TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATTCTGGGCTTCACTTCTGATGAAAGAGTC
  TCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAGAAGCAACGAGAGG
  AGCAGGCTGAGCCAGACGGCACCGAAGTTGCTGACAAGGCTGCCTATCTCCAAAATCTGAACTCTGCTGA
  CCTGCTCAAAGCCCTGTGTTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTG
  CAGCAGGTGTACAACTCAGTAGGCGCCCTAGCCAAAGCCGTCTATGAGAAGATGTTCCTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG
  CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG
  TTCTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATCGAGTGGGAGTTCA
  TTGACTTTGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT
  GGAAAGTCCAACAACTTCCAAAAGCCCAAACCTGCCAAAGGCAAGGTTGAAGCTCACTTCTCCCTGGTGC
  ACTATGCGGGCACTGTGGACTACAACATCGCTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC
  TGTGGTGGGGCTGTACCAGAAGTCTTCAATGAAAACTCTGGCTTACCTCTTCTCTGGGGCAGCAGCAGCT
  GCGGAAGCAGAGTCTGGTGGCGGTGGTGGAAAGAAAGGGGCCAAGAAGAAGGGTTCCTCCTTCCAGACCG
  TGTCAGCCCTCTTCAGGGAGAATCTGAATAAGCTAATGACCAATCTGAGGAGCACTCACCCCCACTTTGT
  ACGGTGCATCATCCCCAATGAAACCAAGACTCCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG
  AGGTGTAACGGGGTGCTGGAGGGCATCCGCATCTGCAGGAAGGGCTTCCCCAGCAGGATCCTCTATGCAG
  ACTTCAAGCAGAGATATAAGGTTTTAAATGCAAGTGCCATCCCTGAGGGACAGTTCATCGATAGCAAGAA
  GGCTTCTGAGAAGCTCCTGGGATCCATTGATATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC
  TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTTATAACCC
  GGACCCAGGCTATGTGCAGAGGGTACTTGGCGAGAGTGGAGTACCAGAAGATGGTGGAGAGAAGAGAGTC
  CATCTTCTGCATCCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTAT
  TTCAAGATCAAACCTCTCTTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGAATTTG
  AAAAAGCTAAAGAAAACCTTGCAAAGGCGGAGGCCAAAAGAAAAGAGCTGGAAGAAAAGATGGTAGCTCT
  GATGCAAGAGAAAAATGACCTGCAGCTCCAAGTTCAGTCTGAAGCAGACAGCTTGGCCGATGCAGAGGAG
  CGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCG
  AGGATGAGGAGGAGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT
  GAAGAAGGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC
  AAGGTGAAAAACCTGACAGAGGAGATGGCAGGCCTGGATGAAACCATCGCCAAGCTGACCAAGGAGAAGA
  AGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT
  GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGATCTTGAAGGCTCTCTGGAACAAGAAAAGAAA
  ATCCGGATGGATCTTGAAAGAGCCAAGAGGAAACTGGAGGGAGACCTGAAACTGGCCCAAGAGTCCACAA
  TGGACGTAGAAAACGATAAACAGCAGCTTGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCT
  GCAGAGCAAGATTGAGGACGAGCAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCC
  CGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT
  CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCA
  AATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTG
  CAGCATGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA
  TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGATGA
  CCTCGCTAGTAACATGGAGGTCATCTCCAAATCTAAGGGAAACCTTGAGAAGATGTGCCGCACACTGGAG
  GACCAAGTGAGTGAGCTGAAGACCAAGGAGGAGGAACAGCAGCGGCTGATCAATGAGCTGACTGCGCAGA
  GGGGGCGTCTGCAGACGGAGTCAGGTGAATACTCACGCCAGCTAGACGAAAAGGATTCATTAGTTTCCCA
  GCTCTCCAGGGGCAAACAGGCATTCACTCAACAGATTGAGGAGCTGAAGAGGCAGCTTGAAGAGGAGATA
  AAGGCCAAGAGTGCCTTAGCTCATGCTCTTCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGT
  ATGAAGAAGAACAGGAAGCCAAGGCTGAGCTGCAGAGGGCCATGTCCAAAGCCAACAGTGAAGTGGCCCA
  GTGGAGGACAAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG
  GCTCAGCGTCTGCAGGATGCTGAGGAGCACGTAGAAGCTGTGAACGCCAAGTGCGCTTCCCTGGAGAAGA
  CGAAGCAGCGGCTGCAGAACGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCAGCCTGCGC
  CGCCCTGGACAAGAAGCAGAGAAACTTTGACAAGATCCTGGCAGAATGGAAACAGAAGTATGAGGAAACC
  CATGCTGAACTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTCAGCACAGAGCTGTTCAAGATTAAGAATG
  CTTATGAGGAATCTTTAGACCATCTCGAAACCTTGAAAAGGGAGAATAAGAATTTGCAACAGGAGATTTC
  TGACCTCACTGAACAGATTGCAGAAGGAGGGAAACGCATCCATGAACTGGAGAAAATAAAGAAGCAAATT
  GAGCAGGAAAAATCTGAGCTCCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA
  AAATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA
  TGAGGAGATCGACCAGCTGAAGAGAAACCACATTAGAGTCGTGGAGTCCATGCAGAGCACGCTGGATGCT
  GAGATTAGGAGCAGGAATGACGCCATCAGGCTCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAAA
  TCCAGCTGAACCACTCCAACCGCATGGCTGCCGAGGCCCTGAGAAACTATAGGAACACACAAGGCATCCT
  CAAGGACACTCAGCTGCACCTGGACGATGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG
  GTTGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACCCTGGAGCAGACGGAGA
  GGAGCAGGAAGATTGCAGAGCAGGAGTTGCTGGATGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA
  CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAGGAC
  ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGATGCCGCCATGATGGCGG
  AGGAGCTGAAGAAGGAGCAGGATACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGT
  GAAGGACCTGCAGCACCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG
  AAACTGGAGGCCAGGGTCCGTGAACTTGAAGGAGAAGTGGAAAATGAACAGAAGCGCAACGTGGAAGCTA
  TCAAGGGTCTGCGCAAACATGAGAGAAGAGTGAAAGAACTCACTTATCAAACTGAGGAAGACCGCAAGAA
  TGTTCTCAGGCTCCAGGACCTTGTGGACAAACTGCAATCAAAGGTCAAGGCCTACAAGAGACAAGCTGAG
  GAAGCGGAGGAACAATCCAATGTCAACCTGGCCAAATTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCG
  AGGAGCGGGCTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAA
  AATCATAAGCGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212099 representing NM_030679
  Red=Cloning site Green=Tags(s)
  
  MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTV
  TVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVV
  AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEA
  TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL
  KAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERV
  SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTV
  QQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ
  FFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL
  GKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAA
  AEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQL
  RCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKV
  FFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLY
  FKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEE
  RCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATEN
  KVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKK
  IRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQA
  RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATL
  QHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLE
  DQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEI
  KAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKL
  AQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEET
  HAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQI
  EQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDA
  EIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAM
  VERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMED
  IVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQ
  KLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAE
  EAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030679
• ORF Size: 5826 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030679.1, NP_109604.1
• RefSeq Size: 6058
• RefSeq ORF: 5829
• Locus ID: 17879
• MW: 223.3 kDa
• Gene Summary: Muscle contraction. [UniProtKB/Swiss-Prot Function]