AU040320 (NM_001035525) Mouse Tagged ORF Clone

CAT#: MR212614

AU040320 (Myc-DDK-tagged) - Mouse expressed sequence AU040320 (AU040320), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AU040320
• Synonyms: A730047D20Rik; AAVR; Kiaa0319l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212614 representing NM_001035525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGAGACTGGGAGTCAAGCCAAGTCCCGCTTCCTGGGTTTTGCCAGGATATTGTTGGCAGACAT
  CAGTGAAGCTGCCGAGAAGCCTGTACCTGCTTTACAGTTTCTTCTGCTTCAGCGTTCTGTGGTTGTCAAC
  AGATGCTGATGAGAGCAGATGCCAACAGGGGAAGACACTTTATGGAGCTGGCTTGAGAACTGAGGGAGAA
  AATCACCTCCGGCTTCTTGCAGGAAGCCTGCCTTTCCACGCCTGTCGGGCTGCCTGCTGCCGGGACTCTG
  CCTGCCACGCTCTATGGTGGCTGGAAGGGATGTGCTTTCAGGCTGACTGCAGTAAGCCCCAGAGCTGCCA
  GCCTTTTAGGACAGACTCTTCCAATTCCATGCTGATCATTTTTCAAAAATCCCAAACTACAGATGATTTG
  GGCCTTCTGCCTGAAGATGATGAACCACATCTTCTGAGGCTAGGCTGGGGCAGGACATCGTGGAGGAGGC
  AGAGCCTTCTTGGGGCTCCCCTCACCCTTTCTGTACCCTCTAGTCACCACCAGAGCTTACTCAGGGATCG
  GCAGAAGAGAGATCTCAGTGTGGTACCTACACATGGAGCGATGCAGCATTCTAAAGTGAATCACTCGGAG
  GAAGCAGGTGCTCTGAGTCCCACCTCTGCAGAGGTCCGCAAAACCATTACAGTTGCCGGTTCCTTCACCA
  GTAACCACACTACACAGACTCCTGAGTGGCCCAAGAATGTGTCCATCCATCCTGAACCATCCGAGCACTC
  CAGTCCTGTATCTGGTACTCCGCAAGTAAAAAGCACTGAGCACAGTCCAACTGATGCCCCTCTGCCAGTG
  GCCCCCTCCTACAGCTATGCCACCCCCACGCCCCAGGCCTCTTCTCAGAGCACCTCAGCACCACACCCAG
  TTGTAAAGGAGCTGGTGGTGTCTGCTGGGAAGAGCGTCCAGATCACCCTGCCTAAGAATGAAGTTCAGTT
  AAATGCCTTCGTCCTTCCAGAAGCAGAGCCAGGAGAAACCTACACCTACGACTGGCAGCTGATCACTCAT
  CCTACAGACTACAGTGGAGAGGTGGAGAGGAAACATTCCCAGAGCCTCCAACTGTCCAAGCTGACTCCAG
  GCCTGTACGAATTCAAGGTGACTGTGGATGGCCAGAATGCCCATGGGGAAGGCTACGTGAATGTGACAGT
  GAAACCAGAGCCCCGTAAGAACCGGCCTCCCGTTGCTGTGGTGTCACCTCAGTTCCAGGAGATCTCGCTG
  CCAACCACTTCTACCATCATTGATGGCAGCCAGAGCACGGATGACGATAAAATTGTCCAGTACCACTGGG
  AAGAGCTTAAGGGGCCCCTGAGAGAAGAGAAGATCTCTGAAGACACAGCCATACTAAAACTTAGTAAGCT
  CGTCCCGGGGAACTACACCTTCAGCTTAACTGTTGTCGACTCTGACGGGGCTACCAACTCCACCACTGCA
  AGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCCAACCAAGTGATCACCC
  TGCCTCAGAACTCCATCACCCTCTTTGGAAACCAGAGCACGGATGACCACGGCATCACCAGCTATGAGTG
  GTCGCTCAGCCCGAGCAGCAAAGGGAAGGTGGTGGAGATGCAGGGAGTTAGAACGCCAGCCCTGCAGCTG
  TCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGGACAACAGGCCACCG
  CCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCAGGCCCAGACAAAGA
  GCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACCAGAGAGTCGTCTCT
  TACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAGCAGTGTCGCCACTG
  TGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGGAACCTACAGAGCCA
  GAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGATCGCTGGGAACGTG
  GTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGACAAGGGGATAGTCA
  GCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCACTCTGACCACCACCC
  TGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGACAGATGCAAAGGGC
  GAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAACCTAGTGGAGATCA
  TCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGCCAGATTGGGGTCCT
  CCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAGATG
  TTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGCAGCCATGCTCAAGA
  GCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGCACAGTCACATGTCA
  GCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTGACCCGTTTTGGATG
  GAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGTGCTCTACGTCATCA
  TTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAGAGGCA
  AAAAGGAAAACCCAAGAGGAAAAGCAGATACAAGATCCTGGATGCCACAGATCAGGAGAGCCTGGAGCTG
  AAACCAACCTCCCGAGCAGGCAGCAAACAGAAAGGCCCCACGCTGAGCAGCAGCCTGATGCATTCTGAAT
  CGGAGCTGGACAGCGACGATGCCATCTTCACATGGCCAGACCGGGAGAAGGGCAAACTACTGGCCCTCCA
  CTGGTGTAGATCCCAAAGCCTGCAGCATATTGGCTGGTGGTGTAGGGCCCCAGTCTGCCATGAAACCTCA
  TCTACCAACAGCACACCTGGCTCTGACTCCGGCTTCTGTAAAGGCGGGAAGAACCTCAGCTCAGACTACA
  GAACCAGGACGAAGTCAGCTTTGGTCCATGGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212614 representing NM_001035525
  Red=Cloning site Green=Tags(s)
  
  MEKRLGVKPSPASWVLPGYCWQTSVKLPRSLYLLYSFFCFSVLWLSTDADESRCQQGKTLYGAGLRTEGE
  NHLRLLAGSLPFHACRAACCRDSACHALWWLEGMCFQADCSKPQSCQPFRTDSSNSMLIIFQKSQTTDDL
  GLLPEDDEPHLLRLGWGRTSWRRQSLLGAPLTLSVPSSHHQSLLRDRQKRDLSVVPTHGAMQHSKVNHSE
  EAGALSPTSAEVRKTITVAGSFTSNHTTQTPEWPKNVSIHPEPSEHSSPVSGTPQVKSTEHSPTDAPLPV
  APSYSYATPTPQASSQSTSAPHPVVKELVVSAGKSVQITLPKNEVQLNAFVLPEAEPGETYTYDWQLITH
  PTDYSGEVERKHSQSLQLSKLTPGLYEFKVTVDGQNAHGEGYVNVTVKPEPRKNRPPVAVVSPQFQEISL
  PTTSTIIDGSQSTDDDKIVQYHWEELKGPLREEKISEDTAILKLSKLVPGNYTFSLTVVDSDGATNSTTA
  SLTVNKAVDYPPVANAGPNQVITLPQNSITLFGNQSTDDHGITSYEWSLSPSSKGKVVEMQGVRTPALQL
  SAMQEGDYTYQLTVTDTAGQQATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSTDDQRVVS
  YLWEQSRGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPVAKIAGNV
  VVTLPTSTAELDGSRSSDDKGIVSYLWTRDETSPAAGEVLNHSDHHPVLFLSNLVEGTYTFHLKVTDAKG
  ESDTDRTTVEVKPDPRKSNLVEIILDVNVSQLTERLKGMLIRQIGVLLGVLDSDIIVQKIQPYTEQSTKM
  LFFVQNDPPHQLFKGHEVAAMLKSELQKQKADFLIFRALEISTVTCQLNCSDHGHCDSFTKRCVCDPFWM
  ENFIKVQLRDGDSNCEWSVLYVIIASFVIVVALGILSWTTICCCKRQKGKPKRKSRYKILDATDQESLEL
  KPTSRAGSKQKGPTLSSSLMHSESELDSDDAIFTWPDREKGKLLALHWCRSQSLQHIGWWCRAPVCHETS
  STNSTPGSDSGFCKGGKNLSSDYRTRTKSALVHGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035525
• ORF Size: 3255 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035525.1, NP_001030602.1
• RefSeq Size: 3697 bp
• RefSeq ORF: 3258 bp
• Locus ID: 100317
• Cytogenetics: 4 D2.2
• MW: 119.9 kDa