Ppl (NM_008909) Mouse Tagged ORF Clone

CAT#: MR215229

Ppl (Myc-DDK-tagged) - Mouse periplakin (Ppl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppl
• Synonyms: AW553870
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215229 representing NM_008909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA
  ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCAGACCAGGTGGAAAGGAACATTGTGGA
  CACCGAGGCCAAGATGCAGAGCGACTTGGCCCGGATGCAAGAGGGGCAGCTCCCTGAGCACCGGGACGCG
  GCCCTGCAGAACGTGTCTGACTCAGAAAAGCTGCTGTACGTGCTGGAGGCAGATTCGGCCATCGCCAAGC
  ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGCGTGACCAACCT
  GCGGGGGAAACACAAGCAGATGTACAGCCTGGCAGTGAAAGAGGCCGACCCGAGAGTCAACTGGGACACA
  CTGGTGGACGAGAAGCTGGACAAGCTGAGCAGCCAGAGCTTTGGGACTGACCTTCCTCTGGTGGATAGCC
  AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA
  GCAGAACAGTGAACTCCAAGCCAAGTATCAGAAGTTGCTGACAGCATCACAGGCACGGCAGCAGCACCTG
  AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC
  GCATGCAGTATGACTGGAGCGACCGCAACCTCGACTACCCCAGCCGCCGCCGCCAGTACGAGAATTTCAT
  CAACCGGAACCTGGAGGCCAAAGAAGAAAGAATCAACAAACTGCACACTGAAGGTGACCAACTGCTGACA
  GCTGAGCACCCAGGGAGGAACTCCATTGAGGCACACATGGAGGCTGTGCATGCTGAGTGGAAGGAGTACC
  TGAACCTGCTCATCTGTGAGGAGAGCCATCTGAAATACATGGAAGACTACCACCAATTTCACAAAGACAT
  GAAGGATGCTCAGGAGCTGCTACGCAAGGTGGACTCAGACTTGAACCAGAAATATAGCCCTGACTTCAAA
  GACCGGTATCAGATAGAGCTGTTGCTTCGGGAACTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG
  ATGTGGTGCGAGGGCTGCAGAGGCGAGGGCAGCAAGTGGTGCCACTCAAGTACCGCCGCGAGACGCCGCT
  CAAGCCCATCCCTGTGGAGGCTCTCTGTGACTTTGAGAGTGACCAGGGCCTCATCTCGCGGGGCTATAGC
  TACACACTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGGCTGCCC
  CAGCCGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTTGCTGACAGCCTGGGCAGCCA
  GTACCGAAGTGTGAGGCAGAAGGCGACTGGGAGCAAACATGCACTCCAGCAGCGGCATGAGGTGCTAAGG
  ACGGAGAACCCTGGAGATGCCTCTGATCTTCAGGGACGACAGCTGCTGGCTGGCTTGGACAAAGTGGCCA
  GTGACCTGGACCGGCAGGAAAAGGCTATCACAGGGATCCTACGGCCACCACTTGAGCAGGGACGGGCAAT
  TGAGGATAGTGCGGAGCGGGCCAAGGGTCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG
  AAGACGCAGTGCACAGCCGAGTGTGAAGCCTTTGTGCAGGCCCTCCCGGCCAGCGGCACCGCACCCTTGC
  TGAAGACCCGGGTAGAAGACACCAACCAGAAATATGAGCGACTAGTATGGTTGCTAGAGGCAGCCCAGGA
  GAAGGTGGACGTGGCCAACCGCCTGGAGAACAGTCTGCAGCGGGGCAGGGAGCTGCTGGCCTCATACGAG
  AACAGGCTGATTCAGGACGACACAATGCCTGAAAGTGGTCATGTGTTGGACAGCAAGAGGCAGGAGCTGG
  AGGCCATGGCTTCTGAGCTGCAGGCCCACAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTACAGGTGGC
  CAAGCAGTGTTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGGCAGGAGGCC
  GAGGTGCACAAGCTCAACCAACGTTTCAACAACCTCAGCCAGCAGGTGGAACGGAGGGCCCAGAGCCTGC
  AGAGTGCCAGGGCTGCCTACGATGAATACTGCAGTGGTTACAACCGTGTGCTCCAGTTTTTGGCCAAGAC
  TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCGGCCAGATGGAGACCAAGCTCAAGAACCAGAAGAAC
  TTGCTTGATGAGCTAGCAAGCAGGGAACAGGAGGTGCAGAAGGTCTATGCAGATTCCCAGCAGTACCAGC
  AAGCGGTGAAGGACTATGAACTTGAAGCAGAGAAGCTAAGGTCCTTGCTGGACCTGGAGAATGGACGGAA
  CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT
  GCTGCCAAGTTCACAGAGGTTAATGCCATCAACAGACAGAGGCTACAGAATTTGGAGTTTGCACTGAATC
  TCTTGAGACAGCAACCAGAAGCAGGAGTGACCCATGAGACCCTACAAGGGGGAAAGCTAAGCTCTGGCAT
  GGAGGAAACATGGAAGATTAAGAAGGAACTGGAGGAGGAGATAGAGCGAAGGCAACAGCTGGAAAATGAG
  GTCAAGAGTGCCCAGGAGGAAATCCAGACCCTGAAGGATCAGGGGCCTCAGGAGTCATTGGTGAGGAAGG
  AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACGCTGGCTGA
  GGAACAGCACAAGAACCAGCTATTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCTCTAGAG
  CAGGAGACCAGGGATGGGGGACAGGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC
  AGGAGGATGAGGTCTTGCAGCTTCGGGAGGAGCTGGAGGCACTGCGGCGCCAGAAAGGCGCCCGAGAGGC
  TGAAGTGCTACTCTTACAGCAGCGTGTAGCAGCCCTGGCTGCTGAGAAGAGCAGGGTGCAGGAAAAGGTC
  ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCCCAACTGGAGGCAGAATACCGGAGGCTGCAGGAGG
  AGCACCAGCGGGAGGGCACACTCAGGGAGAAGCAGGAGGAGGAGCTGAGTTTCCTACAGGCTAAGCTCAG
  GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAGGTAGAGAAA
  GATGCAGCTGTGGAGAGAGAAGTCAATGACCTCACCCGCCAGTATGAGGACGAGGCTGCCAAGGCACGCT
  CTGGCCAGCGGGAGAAGACAGAACTTCTCAGAAAGATATGGGCCCTGGAAGAGGAAAATGCTAAGGTGGT
  GGTACAGGAGAAGGTCCGAGAGATCGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC
  CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGGGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC
  TGGAGGCCCTACGGAACCGTGGACCACAGGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATATAC
  AACTGACCCAGAGACAGAGCAGGAACTCCAGAGGCTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA
  GAAAGGTGTGACCTAGAGATTTACCAGCTGAAGCAGGAGATCCAGGCCCTTAAGGACACCAAGCCACAGG
  TGCAGACTAGAGAGGTAGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA
  GTCTCTGCGTATACAGCTGTCAGAAGAACAGAAGAAACAGGTGGACCTGGAAGGGGAGCGGGCATCCCAG
  GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCACAGGGGAAGGAAAGGGTTGTACGCCAGGAGGTAG
  TACAGTATGAAGATGAGCCAGATTTGCGAGCTGAGGTGACTGCCTTCACAAACAGCATTGATGCTGAGCT
  GCGGCAGATTGATAAACTGCATGTGGAGCTACGGCGGTTACAGCATCGCCGAGCAGAGCTGGAGAGGCAG
  TTGGAGGAGCTGGAGCGGGAGAGGCAGGCACGTAGGGCAGCCGAGCTGGAGGTACAGAGGCTGCAACAGC
  GGCTGGCTGCATTGGAGCAGGAGGAGGCCAAGACTGGTGAGAAGGTGACCCACACACAAAAGGTGGTGCT
  GCAGCAGGACCCACAGCAGACCAGGGAGCATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGGCACCGG
  AGGCAGCTACTGGAGGGTGAGCTTGAGCCGCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGGCAGAGATCA
  AAGAAAAGGTGGTCTTCTCTGAGAGTGTCCAGGTAGAAAAAGGTGACACTGAGCAAGAGATCCAGCGCCT
  CAAGAAGAGCCTGGAAGAGGAGAGCCAGAGCAAGAGGGAGCTGGATTCAGAGGTGACCCGGCTAGAAGCC
  AAGTTATCTGAGCTAGAATTCTATAATTCCAAGTCATCCAAGGAACTGGACTTCCTGAGAGAAGAAAACC
  ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC
  AGCAACAGAAACTCGAGATCTGAAGAACATAACCACGATAGACTCTGGGACCCACCTAAACTCTAGGCTG
  TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC
  TACAGAGGCGCCTGGGCTCTGTGGCTGTCAAGAGAGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT
  GGTCATAGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCTCACCGGGCTGGGCTTATCGACTGG
  AAAATGTTTGTGAAGCTCAGAAGCCAGGAGTGCGACTGGGAGGAGATATCAGTGAAAGGTCCTAATGGGG
  AGTCATCAGTAATTCATGACAGGAAGTCAGGCAAGAAGTTCTCCATTGAGGACGCTCTTCAGAGTGGCAG
  GCTAACCGCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG
  TCTGGGCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215229 representing NM_008909
  Red=Cloning site Green=Tags(s)
  
  MHSLFRKRNKGKYSPTVQTRSISNKELSDLIEQLQKNADQVERNIVDTEAKMQSDLARMQEGQLPEHRDA
  ALQNVSDSEKLLYVLEADSAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQMYSLAVKEADPRVNWDT
  LVDEKLDKLSSQSFGTDLPLVDSQVEQHNIFHNEVKAIGPHLAKDKEQNSELQAKYQKLLTASQARQQHL
  SSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHTEGDQLLT
  AEHPGRNSIEAHMEAVHAEWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFK
  DRYQIELLLRELDDQEKALDKYEDVVRGLQRRGQQVVPLKYRRETPLKPIPVEALCDFESDQGLISRGYS
  YTLQKNNGESWELTDSTGKKLAAPAVCFIIPPTDPEALALADSLGSQYRSVRQKATGSKHALQQRHEVLR
  TENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAIEDSAERAKGLKNITNELLQIEPE
  KTQCTAECEAFVQALPASGTAPLLKTRVEDTNQKYERLVWLLEAAQEKVDVANRLENSLQRGRELLASYE
  NRLIQDDTMPESGHVLDSKRQELEAMASELQAHKSLLGEVEQNLQVAKQCSSSLASRFQEHCPDLERQEA
  EVHKLNQRFNNLSQQVERRAQSLQSARAAYDEYCSGYNRVLQFLAKTPSYEPQETDSLGQMETKLKNQKN
  LLDELASREQEVQKVYADSQQYQQAVKDYELEAEKLRSLLDLENGRNSHVNKRARLQSPAAKVKEEEAAL
  AAKFTEVNAINRQRLQNLEFALNLLRQQPEAGVTHETLQGGKLSSGMEETWKIKKELEEEIERRQQLENE
  VKSAQEEIQTLKDQGPQESLVRKEVLKKVPDPALEESFQQLQQTLAEEQHKNQLLQEELGALQLRLQALE
  QETRDGGQEYVVKEVLRIEPDRAQEDEVLQLREELEALRRQKGAREAEVLLLQQRVAALAAEKSRVQEKV
  TEREVVKLQNDPQLEAEYRRLQEEHQREGTLREKQEEELSFLQAKLRRLEKERAMAEGKITVKEVLKVEK
  DAAVEREVNDLTRQYEDEAAKARSGQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLR
  LELVEQERKFRGAEEQLKSYQSELEALRNRGPQVEVKEVTKEVIKYTTDPETEQELQRLREEIMDKTRLI
  ERCDLEIYQLKQEIQALKDTKPQVQTREVVQEILQFQEDPQTKKEVESLRIQLSEEQKKQVDLEGERASQ
  EEKIKRKEEELAQGKERVVRQEVVQYEDEPDLRAEVTAFTNSIDAELRQIDKLHVELRRLQHRRAELERQ
  LEELERERQARRAAELEVQRLQQRLAALEQEEAKTGEKVTHTQKVVLQQDPQQTREHALLRAQLEEERHR
  RQLLEGELEPLRRKLAALEKAEIKEKVVFSESVQVEKGDTEQEIQRLKKSLEEESQSKRELDSEVTRLEA
  KLSELEFYNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEIEMAATETRDLKNITTIDSGTHLNSRL
  WSLEKELDDLKKMSKDKDLEIDELQRRLGSVAVKREQRENHLRRSIVVIDPDTGRELSPEEAHRAGLIDW
  KMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEDALQSGRLTAAQYDRYVNKDMSIQELAVLV
  SGQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008909
• ORF Size: 5262 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008909.1, NM_008909.2, NP_032935.2
• RefSeq Size: 6277
• RefSeq ORF: 5265
• Locus ID: 19041
• MW: 203.8 kDa
• Gene Summary: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. [UniProtKB/Swiss-Prot Function]