Gls (NM_001081081) Mouse Tagged ORF Clone

CAT#: MR215287

Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gls
• Synonyms: 6330442B14; AI314027; B230365M23Rik
• Vector: pCMV6-Entry2
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215287 representing NM_001081081.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGCGGCTGCGAGGCTCGGCGATGCTACGGGAGCTGCTCTTAAGGCCGCCCGCCGCCGTCGGAGCC
  GTCCTGCGGCGCGCGCAGCCCCTCGGCACGCTGTGCCGGCGCCCCCGGGGCGGGAGCCGGCCGACCGCT
  GGCCTGGTGGCCGCTGCGCGACTCCACCCGTGGTGGGGCGGGGGCGGCCGCGCGAAGGGCCCCGGCGCG
  GGCGGCCTGTCCAGTTCGCCCTCGGAGATCCTACAGGAGCTGGGGAAGGGGGGTACGCCGCCACAGCAG
  CAGCAGCAGCAGCAGCAGCAGCCAGGGGCGTCGCCACCCGCAGCCCCGGGCCCCAAGGACAGCCCGGGG
  GAGACCGACGCGTTCGGCAACAGCGAGGGCAAGGAGATGGTGGCCGCGGGCGACAATAAAATAAAACAG
  GGTCTGTTACCTAGCTTGGAAGATTTGCTGTTCTATACAATTGCAGAAGGACAAGAAAAGATACCTGTT
  CACAAGTTTATTACAGCACTCAAATCTACAGGATTGCGAACATCTGATCCCAGGTTGAAAGAGTGTATG
  GATATGTTAAGATTAACTCTTCAGACAACGTCAGATGGTGTCATGCTAGACAAAGATCTTTTTAAAAAG
  TGTGTTCAAAGCAACATTGTTTTGTTGACACAAGCATTTAGAAGAAAGTTTGTCATTCCTGACTTTATG
  TCTTTTACCTCACACATCGATGAGTTATATGAAAGTGCTAAAAAGCAGTCTGGAGGGAAGGTTGCTGAT
  TATATTCCTCAGCTGGCCAAATTCAGTCCTGATTTGTGGGGTGTATCTGTCTGTACTGTAGATGGGCAA
  AGGCATTCTATTGGAGATACCAAAGTTCCTTTTTGTCTTCAGTCCTGTGTAAAACCCCTGAAATATGCA
  ATTGCTGTTAATGACCTGGGAACTGAGTATGTACATCGCTATGTTGGGAAGGAGCCAAGTGGATTAAGA
  TTCAACAAACTCTTTTTGAATGAAGATGATAAACCACATAATCCTATGGTAAATGCTGGAGCAATTGTT
  GTGACTTCTCTAATAAAGCAAGGAGTAAATAATGCTGAGAAGTTTGACTACGTGATGCAATTTTTGAAT
  AAGATGGCTGGTAATGAATATGTTGGATTCAGTAATGCAACGTTTCAGTCTGAACGAGAAAGTGGAGAT
  CGAAATTTTGCAATAGGATATTACTTAAAAGAAAAGAAGTGTTTTCCAGAAGGCACAGACATGGTTGGG
  ATACTAGATTTTTACTTCCAGCTGTGCTCTATTGAAGTGACATGTGAATCAGCAAGTGTGATGGCTGCC
  ACCTTGGCTAATGGTGGTTTCTGCCCAATTACTGGTGAAAGAGTCCTCAGTCCTGAGGCAGTTCGGAAT
  ACACTGAGCTTGATGCATTCTTGTGGCATGTATGACTTCTCAGGGCAGTTTGCGTTCCATGTTGGTCTT
  CCTGCAAAATCTGGAGTTGCTGGGGGTATTCTTTTAGTTGTCCCCAACGTCATGGGCATGATGTGTTGG
  TCTCCTCCTCTTGACAAGATGGGCAACAGTGTTAAGGGAATTCACTTTTGTCACGATCTTGTTTCTCTG
  TGTAACTTCCATAACTATGATAATTTGAGACACTTTGCAAAAAAACTTGATCCTCGGAGAGAAGGAGGT
  GATCAAAGGGTGAAGTCGGTGATAAACCTTCTGTTTGCCGCATACACTGGAGATGTGTCTGCCCTCCGA
  AGGTTTGCTCTGTCAGCCATGGACATGGAGCAGCGGGATTATGACTCCAGAACAGCCCTCCATGTCGCA
  GCAGCAGAGGGTCATGTTGAAGTTGTCAAGTTTTTGCTGGAAGCTTGCAAAGTAAACCCTTTCCCCAAG
  GACAGGTGGAATAATACCCCCATGGATGAAGCACTACACTTTGGACACCATGATGTTTTTAAAATCCTT
  CAGGAATACCAAGTTCAGTACACACCTCAAGGGGATTCTGATGATGGAAAGGGAAACCAGACTGTCCAC
  AAGAATCTCGACGGGTTGCTA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR215287
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRPTAGLVAAARLHPWWGGGGRAKGPGA
  GGLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQ
  GLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKK
  CVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQ
  RHSIGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIV
  VTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVG
  ILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGL
  PAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGG
  DQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPK
  DRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDDGKGNQTVHKNLDGLL
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081081
• ORF Size: 2022 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081081.2, NP_001074550.1
• RefSeq Size: 4974 bp
• RefSeq ORF: 2025 bp
• Locus ID: 14660
• UniProt ID: D3Z7P3
• Cytogenetics: 1 26.86 cM
• MW: 74 kDa
• Gene Summary: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.[UniProtKB/Swiss-Prot Function]