Mcm3 (NM_008563) Mouse Tagged ORF Clone

CAT#: MR215631

Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcm3
• Synonyms: AL033361; C80350; Mcmd; P1; P1-MCM3; p1.m
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215631 representing NM_008563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTTCCTGG
  ATGACGAGGAGGACCAAGGCATTTACCAGAACAAGGTTCGGGAACTGATCAGTGACAATCAGTATCGGCT
  GATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCTCCTGAACAATGCGTTT
  GAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGCCAAGC
  AGTATGAGGAGTTCTACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTCTCTCCCCGGACTCTCAC
  TTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTACTAAATGCTCTCTGGTTCGCCCCAAA
  GTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCTCACCA
  CCCTAGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGA
  GTATGGCCTGTCTGTCTACAAGGACCATCAGACCATCACCATCCAGGAGATGCCAGAGAAGGCCCCTGCT
  GGTCAGCTCCCTCGCTCTGTGGATGTCATTCTAGACGATGACCTGGTGGATAAAGTGAAGCCTGGTGACA
  GAATACAGGTGGTGGGGACCTACCGCTGCCTTCCAGGGAAGAAGGGCTGCTACACCTCGGGGACCTTCAG
  GACCGTCCTGATTGCCTGTAATGTGAAGCAGATGAGTAAGGATATTCAGCCTGCATTCTCTGCGGACGAT
  ATAGCCAAGATCAAGAAGTTCAGCAAAACTCGTTCCAAGGATGTCTTTGAGCAGCTGGCCCGGTCGTTAG
  CCCCGAGCATCCATGGGCATGACTATGTCAAGAAGGCAATCCTCTGCTTGCTCCTGGGAGGGGTGGAGCG
  GGAACTTGAAAACGGCAGCCACATCCGTGGAGACATCAATATTCTTCTAATAGGTGACCCCTCCGTTGCC
  AAGTCCCAGCTTCTGCGGTACGTGCTTTGCACGGCGCCCAGGGCCATTCCCACCACGGGACGGGGCTCCT
  CTGGAGTGGGTCTCACAGCTGCTGTCACTACAGACCAGGAAACAGGGGAGCGCCGCCTGGAAGCTGGTGC
  TATGGTCCTGGCTGACCGTGGAGTGGTTTGCATTGATGAGTTTGACAAGATGTCTGACATGGACCGCACA
  GCCATCCACGAGGTGATGGAGCAGGGTCGAGTGACCATTGCAAAGGCTGGCATCCATGCCCGGCTCAATG
  CCCGCTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGATCAGTATAAGACGCCCATGGA
  GAACATTGGGCTGCAGGACTCACTGCTGTCTAGATTTGACCTGCTCTTCATCATGCTGGATCAGATGGAT
  CCTGAACAGGATCGGGAGATCTCAGACCACGTCCTGAGGATGCACCAATACAGGGCCCCGGGGGAGCAGG
  ACGGTGATGCTTTGCCATTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCTGACTTTACCCAGGA
  TGACCAGCAAGATACCCGCATTTATGAGAAGCATGACAGCCTTCTACATGGGACCAAGAAGAAAAAGGAG
  AAGATGGTGAGCGCAGCTTTTATGAAGAAGTACATTCACGTGGCCAAAATTATCAAGCCCACCCTGACAC
  AGGAGTCAGCAGCCTACATTGCAGAAGAATACTCACGCCTGCGCAGCCAGGACAGTATGAGCTCAGACAC
  TGCCCGGACATCTCCAGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATGCGAAG
  GCCCGGATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCCGTGGAGCTGGTTCAGTATGCTTATT
  TCAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGGCAAGTGAGGATGAATCAGACCTAGAAGATGA
  AGAGGAAAAGAGCCAGGAAGACACTGAGCAGAAGCGGAAAAGGAGGAAGACTCATGCCAAGGATGGAGAG
  TCCTATGACCCTTACGACTTCAGTGAAGCAGAGACGCAGATGCCTCAAGTGCACACCCCAAAGACTGACG
  ATTCCCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGAAAGTGGAACTCAGTGAGCCCAG
  GCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCATGAGCAGTCTGTGGGCATGCTC
  CACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCGTTCTCCTCAGAGGAAATCCAGGCTTGCCTGA
  GCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215631 representing NM_008563
  Red=Cloning site Green=Tags(s)
  
  MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVSVNDLRRKNEKRANRLLNNAF
  EELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK
  VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA
  GQLPRSVDVILDDDLVDKVKPGDRIQVVGTYRCLPGKKGCYTSGTFRTVLIACNVKQMSKDIQPAFSADD
  IAKIKKFSKTRSKDVFEQLARSLAPSIHGHDYVKKAILCLLLGGVERELENGSHIRGDINILLIGDPSVA
  KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
  AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD
  PEQDREISDHVLRMHQYRAPGEQDGDALPLGSSVDILATDDPDFTQDDQQDTRIYEKHDSLLHGTKKKKE
  KMVSAAFMKKYIHVAKIIKPTLTQESAAYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK
  ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKASEDESDLEDEEEKSQEDTEQKRKRRKTHAKDGE
  SYDPYDFSEAETQMPQVHTPKTDDSQEKTDDSQETQDSQKVELSEPRLKAFKAALLEVFQEAHEQSVGML
  HLTESINRNREEPFSSEEIQACLSRMQDDNQVMVSEGIVFLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008563
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008563.1, NM_008563.2, NP_032589.1
• RefSeq Size: 2886
• RefSeq ORF: 2439
• Locus ID: 17215
• MW: 92 kDa
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. [UniProtKB/Swiss-Prot Function]