B4galt2 (NM_017377) Mouse Tagged ORF Clone

CAT#: MR215821

B4galt2 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 (B4galt2)

Product Images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

• 

  Coomassie blue staining of purified B4galt2 protein (Cat# TP515821). The protein was produced from HEK293T cells transfected with B4galt2 cDNA clone (Cat# MR215821) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4galt2
• Synonyms: Ggtb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215821 representing NM_017377
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGACTGCTGGGGGGGACGCTGGAGCGGGTCTGCAAGGCTGTGCTCCTTCTCTGCCTGCTGCACT
  TCCTTGTGGCCGTCATCCTCTACTTTGACGTCTATGCCCAGCACCTGGCCTTTTTCAGCCGCTTCAGTAC
  CCGAAGCCCAGCCCATGCCCTCTACCCTGCAGCCAGCAGCAGTACCAACTGCTCTCGGCCCAACGCCACT
  GCCGCTAGCTCCGGGCTGCCTGAAGTGCCCAGTGCCAGGCCTGGTCCCACAGCTCCAGTCATTCCGCCCT
  GTCCTGACGTGCCGCCTGGTCTTGTGGGCCGAGTGGTCATCGAATTCACCTCACCCATGCCTCTGGAACG
  GGTGCAGAGGGAAAATCCAGGCGTGCTCCTGGGCGGCCGCTATTCTCCACCCGACTGCACCCCAGCCCAA
  ACGGTGGCAGTCATCATCCCCTTTAGACACCGGGAGCACCACCTACGCTATTGGCTCCACTATCTGCACC
  CCATGTTGAGGCGGCAGCGGCTGCGCTACGGTGTCTATGTCATCAACCAGCATGGCGAGGAGACCTTCAA
  CCGAGCCAAGCTGCTCAACGTGGGCTTCCTAGAGGCACTGAAGGAGGATGCTGCCTATGACTGCTTCATC
  TTCAGTGATGTGGACCTGGTCCCTATGGATGACCGCAATCTGTACCGTTGTGGTGACCAGCCCCGCCACT
  TTGCCATCGCCATGGACAAGTTTGGCTTCCGGTTGCCCTATGCTAGCTACTTTGGAGGTGTGTCGGGCCT
  GAGCAAGGCCCAGTTTCTGAGGATCAATGGCTTTCCCAACGAGTACTGGGGCTGGGGTGGCGAGGATGAT
  GACATCTTCAACCGGATCTCCCTGACTGGGATGAAGATCTCACGCCCGGATGTCCGGATTGGCCGCTACC
  GCATGATCAAGCACGACCGGGACAAACATAACGAGCCCAACCCTCAGAGGTTTAATAAGATTCAAAACAC
  AAAGATGAGCATGAAGTGGGACGGCATTGGATCCGTGCGGTACCGAGTCTTGGAAGTGTCTCGGCAACCG
  CTCTTCACCAACATCACAGTGGACATTGGACAGCCCATGTCGTGGCTCACTCAAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215821 representing NM_017377
  Red=Cloning site Green=Tags(s)
  
  MSRLLGGTLERVCKAVLLLCLLHFLVAVILYFDVYAQHLAFFSRFSTRSPAHALYPAASSSTNCSRPNAT
  AASSGLPEVPSARPGPTAPVIPPCPDVPPGLVGRVVIEFTSPMPLERVQRENPGVLLGGRYSPPDCTPAQ
  TVAVIIPFRHREHHLRYWLHYLHPMLRRQRLRYGVYVINQHGEETFNRAKLLNVGFLEALKEDAAYDCFI
  FSDVDLVPMDDRNLYRCGDQPRHFAIAMDKFGFRLPYASYFGGVSGLSKAQFLRINGFPNEYWGWGGEDD
  DIFNRISLTGMKISRPDVRIGRYRMIKHDRDKHNEPNPQRFNKIQNTKMSMKWDGIGSVRYRVLEVSRQP
  LFTNITVDIGQPMSWLTQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017377
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017377.1, NM_017377.2, NM_017377.3, NM_017377.4, NM_017377.5, NP_059073.1
• RefSeq Size: 2432
• RefSeq ORF: 1110
• Locus ID: 53418
• MW: 42.4 kDa