Cep250 (NM_001130000) Mouse Tagged ORF Clone

CAT#: MR216165

Cep250 (Myc-DDK-tagged) - Mouse centrosomal protein 250 (Cep250), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cep250
• Synonyms: AW490617; B230210E21Rik; Cep2; Inmp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216165 representing NM_001130000
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAGGGAGCCCCGGGTTGAACATGAAGCCCCAGTCCTTGCAGCTGGTGCTGGAGGGGCAGGTGC
  TGGCGCTGCAGCAGCAGATGGCCGAGAATCAGGCTGCCTCCTGGCGGAAACTGAAGAACTCCCAAGAGGC
  CCAGAAGAGACAAGCAACCCTCGTGAGGAAGCTGCAGGCCAAGGTTTTACAGTACCGGAGCTGGTGTCAA
  GACCTGGAGAAGCGACTAGAAGCCACTGGGGGGCTGATCCCTCAGCGCTGGGAGAGTGTAGAAGAGCCCA
  ACCTGGAACAGTTGCTGATCCGATTGGAGGAGGAGCAGCAGAGGTGTGAGAGTCTAGTGGAGGTGAACAC
  CGAGCTTCGGCTGCACATGGAGAAAGCCGATGTGGTGAATAAAGCCTTGCAGGAGGACGTGGAAAAACTG
  ACAGTGGACTGGAGTCGGGCCCGAGATGAGTTAGTGAGGAAGGAGAGCCAGTGGCGGATGGAGCAGGAGT
  TCTTCAAGGGCTATTTGAGAGGTGAACACGGTCGTCTTCTCAATCTATGGCGAGAGGTGGTAACCTTCCG
  ACGTCACTTCCTGAAAATGAAGTCAGCTACTGACAGAGACCTGACAGAGCTAAAGGCTGAACACGCAAGG
  CTTTCAGGGTCCCTGCTGACCTGCTGTCTGCGCCTGACCCTGAGAGCACAGTCTCGGGAGTCCAGTGGAT
  CTGGGAGAACAGAGGAGAGTGAGCCAGCCCGGCTGCTGCTGCTCGTCGCTAAGACCCAGGCGCTGGAGAA
  GGAAGCCCATGAGAAGAGCCAGGAGTTAATGCAGCTCAAGAGTCACGGGGATCTAGAGAAGGCAGAGCTG
  CAGGACCGAGTGACGGAACTCTCTGCTCTGCTAACCCAGTCTCAGAAGCAAAATGAAGATTATGAGAAGA
  TGGTAAAGGCTCTAAGAGAAACAATGGAGATCCTGGAGACAAACCATGCAGAGTTAATGGAGCATGAGGC
  ATCTCTCAGTAGGAATGCTCAAGAGGAAAAGTTGTCTCTGCAGCAGGTGATCAAGGCTATAACCCAGGCA
  CTGGCCTCAGTGGAAGAAGAAGACACTGTGACCCAGTCCTCAGGCCATGAGGACTCGCTGCAGTCAGACT
  GTAATGGCCTTTCCCAGTTTGATCCCCAGGACCCAGACAGGGCTCTTACCCTGGTGCAGTCAGTGCTGAC
  TCGGAGACAGCAGGCTGTGCAGGACCTCAGGCAGCAGCTTTCAGGCTGCCAGGAGGCTATGAGCTTCTTG
  CAGCAGCAGCACGATCAGTGGGAGGAAGAGGGCAGGGCCCTGAGGGAAAAGCTACAGAAGCTCACTGGGG
  AGCGGGATGCTCTGGCAGGCCAGACTGTGGGCCTTCAGGGAGAGGTGGACTCTCTCACTGGGGAGCGGGA
  TGCTCTGGCAGGCCAGACTGTGGACCTTCAGGGAGAGGTGGACTCTCTCAGCAGGGAGCGGGAGCTGCTT
  CAGAAGGCCAGAGGAGAGCTGCAGCAGCAACTGGAAGTGCTGGAGCAGGAAGCGTGGCGACTGCGGAGGA
  TGAACATGGAGCTGCAGCTGCAGGGGGACTCAGCTCAAGGGGAGAAGCTGGAGCAGCAGGAGGAGTTGCA
  CTTGGCTGTCCGGGAGAGGGAGCGCCTTCAGGAGACGCTGGTGGGCCTGGAAGCCAAGCAGTCAGAATCA
  CTAAGTGAGCTGCTCACTCTTCGAGAAGCCCTGGAGTCAAGTCGCCTAGAAGGGGAGTTACTGAAGCAGG
  AGCGAGTGGAAGTGGCTGCAGCCTTGGCCAGGGCAGAACAGTCCATTGTGGAGCTGTCAGGTTCTGAGAA
  CAGCCTGAAGGCAGAGGTAGCTGATCTTCGAGCTGCAGCTGTCAAGCTTGGGGCCTTAAACGAGGCTTTG
  GCTTTAGATAAAGTTGAACTGAACCAGCAGCTTCTCCAGTTGGAGCAGGAGAACCAGTCTCTATGCAGCA
  GAGTGGAGGCCGCAGAGCAGCTGAGAAGCGCTTTGCAGGTGGACCTGGCGGAGGCAGAAAGGCGCAGGGA
  AGCCCTGTGGGAGAAGAAAACTCAGCTGGAGACTCAGCTGCAGAAGGCAGAGGAAGCGGGGGCTGAGCTA
  CAGGCAGAGCTGAGGGGCACCCGAGAAGAAAAGGAAGAGCTTAAAGATAAATTAAGTGAGGCACATCACC
  AGCAAGAAACAGCTACAGCTCACCTAGAACAGCTGCATCAGGACGCAGAAAGACAGGAAGAAACACTTGC
  TAGGGCAGTCCAGGAGAAGGAAGCCCTAGTACGGGAGAGGGCAGCTCTTGAGGTGCGCCTGCAGGCTGTG
  GAGCGGGACCGACAGGACCTCACTGAACATGTCCTGGGGCTCAGGTCAGCCAAGGAGCAACTGGAGAGCA
  ATCTATTTGAGGCCCAACAGCAAAATTCTGTGATACAGGTCACCAAGGGGCAGCTGGAGGTCCAGATTCA
  AACTATTATCCAAGCCAAGGAAGTAATTCAAGGGGAAGTGAAATGCCTGAAGCTGGAACTGGATGCTGAG
  AGGACCCGGGCAGAGCAGGAGTGGGATGCAGTAGCCAGGCAGCTAGCCCAAGCTGAGCAGGAGGGGCAAG
  CATCTCTGGAGCGACAGAAGGTGGCCCACGAGGAGGAGGTGAACAGGCTGCAAGAGAAATGGGAGAAAGA
  GCGCTCCTGGCTGCAGCAGGAGCTGGATAAGACCCTGGAAACCCTCGAGAGGGAGAGAGCAGAGCTGGAG
  ACAAAGCTGAGAGAGCAGCAGACAGAAATGGAAGCCATCCGTGCACAGAGGGAAGAGGAACGAAGCCAGG
  CTGACAGCGCCCTGTATCAAATGCAGCTAGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGACACTGCT
  GAGGACCCAGAAGGAGCTGGCAGATGCTAGTCAACAGCTGGAAAGGCTGAGACAGGACATGAAGATTCAG
  AAGTTAAAGGAACAGGAGACCACTGGCATGCTGCAGGCCCAGCTTCAGGAAACACAGCAGGAGCTGAAGG
  AGGCAGCCCAGCAGCACAGGGACGACCTTGCTGCCTTCCAGAAGGACAAGCTGGACCTGCAGAAGCAGGT
  AGAGGACCTAATGTCTCAGCTGGTAGCCCACGATGACTCCCAGAGGCTGGTGAAAGAAGAGATTGAAGAG
  AAGGTGAAAGTGGCTCAAGAATGCAGCCGGATTCAGAAGGAACTGGAGAAAGAGAACGCCAGCCTGGCTC
  TGTCGCTGGTGGAAAAGGAAAAACGACTTCTCATTTTACAAGAAGCTGACTCTGTAAGACAGCAGGAGCT
  GAGCTCCTTGCGCCAGGACATACAGGAGGCCCAGGAAGGGCAAAGAGAGCTGGGTGTACAGGTGGAACTA
  TTGAGGCAGGAGGTGAAGGAAAAGGAGGCTGACTTTGTGGCCCGGGAAGCACAGCTGCTGGAGGAGTTGG
  AGGCCTCACGGGTTGCAGAGCAGCAACTTCGAGCGTCTTTGTGGGCCCAGGAAGCCAAGGCAACCCAGCT
  GCAGCTGCAGCTGCGCAGCACTGAGAGCCAGCTAGAGGCATTGGTTGCAGAACAACAGCCGGAGAACCAG
  GCACAGGCACAGCTAGCTAGCCTCTGCTCTGTTCTGCAGCAGGCCCTGGGGTCTGCATGTGAGAGTAGGC
  CTGAGCTGAGGGGTGGAGGCGACTCTGCTCCTACCTTATGGGGCCCTGATCCAGATCAGAATGGAGCTTC
  CAGACTCTTTAAGAGATGGTCGCTCCCAACGGCACTGTCACCGGAGGCTGTGGCACTAGCTCTCCAGAAG
  CTCCATCAGGACGTGTGGAAGGCTCGGCAGGCCCGGGATGACCTAAGGGACCAGGTCCAGAAGCTGGTGC
  AGCGCCTGACTGACACGGAGGCCCAGAAGAGCCAGGTTCACTCGGAGCTGCAGGACCTGCAGAGACAGCT
  CTCTCAGAGCCAGGAAGAGAAATCCAAGTGGGAAGGGAGGCAGAACTCCCTGGAGTCTGAGCTGAGGGAC
  TTGCACGAGACCGCGGCATCCTTACAGAGTCGCCTCCGGCAGGCAGAACTGCAAAAAATGGAAGCTCAGA
  ATGATCGAGAGTTGCTTCAGGCATCCAAGGAGAAGCTGTCAGCCCAGGTTGAACATCTGCAAGCATGTGT
  TGCAGAAGCCCAGGCTCAGGCAGATGCAGCTGCTGTCCTGGAAGAAGATCTGCGAACTGCTCGCTCTGCA
  CTGAAACTGAAAAACGAGGAGCTAGAGAGTGAACGGGAGAGAGCCCAGGCTTTACAAGAACAGGGTGAGC
  TGAAGGTGGCTCAGGGGAAAGCTTTGCAGGAGAACCTAGCCCTGCTGGCACAGACCCTGTCTAACAGAGA
  GCGGGAGGTCGAGACGTTGCAGGCTGAGGTTCAGGAACTGGAGAAGCAGCGGGAGATGCAGAAGGCTGCT
  CTGGAGCTGCTCTCCCTCGACCTGAAGAAGCGCAGTCGGGAGGTAGACCTGCAACAAGAACAGATTCAGG
  AGCTGGAGCAGTGCAGGTCAGTCTTAGAGCACTTGCCCATGGCCGTCCAAGAGCGAGAGCAGAAACTGAG
  TGTGCAGCGGGACCAGATCAGGGAACTGGAGAACGATCGGGAGGCCCAGAGGAGTGTCCTGGAACACCAG
  CTTCTGGACCTGGAGCAGAAGGCTCAAGTGATTGAGTCCCAGAGGGGACAGATTCAGGATCTGAAAAAGC
  AACTGGGGACACTGGAATGCCTGGCCCTGGAACTGGAGGAAAGCCATCACAAAGTGGAGAGCCAACAGAA
  GATGATCACAGAACTAGAGGGCCAGAGGGAAATGCAGAGGGTGGCTCTAACCCACCTCACACTGGACCTG
  GAAGAAAGGAGCCAGGAGCTGCAGGCACAAAGCAGCCAGCTCCATGAGCTGGAGAACCATAGCACCCATC
  TGGCAAAAGAGCTGCAGGAGAGGGACCAGGAGGTGACGTCCCAACGCCAGCAGATCGATGAGCTGCAGAA
  GCAGCAGGAACAGCTGGCTCAGGCCCTGGAGAGGAAAGGCCAGGAGCTGGTGCTGCAGAAGGAAAGGATT
  CAGGTTCTGGAAGATCAGAGGACGCTGCAGACCAAGATCTTAGAGGAGGACCTAGAACAGATCAAGCATT
  CCTTGAGAGAACGCAGCCAGGAGCTGGCCTCTCAGTGGCAGCTGGTGCATGAGCGTGCCGACGATGGAAA
  GAGCCCGAGTAAAGGCCAGCGAGGGAGCCTGGAGCACTTGAAGCTGATCCTGCGTGATAAGGAGAAGGAG
  GTAGAGTGCCAGCAGGAGCGCATCCAGGAACTTCAGGGCCACATGGGCCAGCTGGAACAGCAGCTGCAGG
  GTCTGCACAGGAAGGTGGGGGAGACTAGCCTGCTCCTGACCCATCGAGAGCAGGAGACAGCAACCCTGCA
  GCAGCATCTCCAGGAAGCCAAGGAACAAGGGGAACTGAGAGAGCAGGTACTTCAGGGTCAGCTGGAGGAG
  GCCCAGAGAGATCTGGCCCAGAGGGACCATGAACTAGAGACCCTCCGGCAGGAAAAACAGCAGACCCAGG
  ACCAGGAGGAAAGCATGAAGCTAAAGACAAGTGCTCTGCAGGCCGCTCTGGAGCAGGCCCATGCGACACT
  GAAGGAGCGTCAGGGGGAGCTGGAAGAACACAGGGAACAGGTGCGAAGGCTCCAGGAAGAACTAGAAGTG
  GAGGGACGGCAGGTACGGGCCCTGGAGGAGGTGTTGGGGGACCTAAGGGCTGAGTCTCGGGAGCACGAGA
  AGGCTGTGCTGGCCCTTCAACAACGGTGTGCTGAGCAGGCCCAGGAACATGAGGCAGAAGCCAGGACCCT
  GCAGGACAGCTGGCTGCAGGCCCAGGCCACTCTCACAGAACAAGAACAAGAGCTAGCGGCACTGCGAGCG
  GAAAACCAGTATTCCCGCCGACAGGAAGAGGCTGCAGTTAGCCAGGCAGAGGCGCTGCAGGAGGCCCTCA
  GCAAAGCCCAGGCTGCCCTGCAGGAGAAAGAGCAGAGTCTGCTTGAACAGGCTGAGTTGAGCCACACCCT
  GGAGGCCAGCACTGCCGCCTTGCAAGCTACCCTGGACACCTGCCAGGCAAGTGCCCGGCAGCTGGAGGAG
  GCCCTGAGGATTCGGGAAGGTGAGATCCAGGCCCAGGCTCTCCAACACCACGAGGTCACGCAGCACCTCC
  AGCAGGAACTTTGCCAGAAGAAAGAAGAGCTGAGGCAGCTACTAGAGAAGGCTGGGGCCCGGAGGAGTCA
  AGAAAATGGAATCCAAGAGAAGCAAAGTCTGGAGCAAGAGAGACAAGAGGAAACAAGGAGGCTCCTTGAG
  AGCCTAAAGGAGCTACAGCTGACTGTAGCCCAAAGGGAAGAAGAGATTCTGATGCTGAGGGAGGCCTCGT
  CCCCACGCCACAGAGCCCTCCCAGCCGAGAAGCCAGCTCTGCAACCGCTCCCAGCACAACAGGAGTTAGA
  GCGGCTGCAGACTGCCCTAAGGCAAACAGAGGCCAGGGAGATTGAATGGAGGGAGAAGGCTCAGGACCTG
  GCTCTGTCCCTGGCCCAAAGCAAGGCCAGCATCAGCAGTCTGCAGGAGATAACCATGTTCCTACAAGCCT
  CTGTTCTAGAGCGGGAATCAGAACAACAAAGACTACAGGAGGAGTTGGTACTCAGCAGACAAGCTCTGGA
  AGAGCAGCAGTCAGGTGGCCCACACTCAACCAGCAGAGCAGACCAGGGACCAAAAGTGGGACAGGGAAGC
  CAGTCTGGAGAGGTAGAGACTGAGCCTAGTCCTGGAGTGGAGGAGAAGGAACGGTTGACACAGAGGCTTG
  AACGTCTGCAGCAAGCGGTGGCAGAGCTGGAAGTTGACCGAAGCAAGCTGCAGTGCCACAATGCGCAGCT
  GCGGACCGCCTTGGAGCAGGTGGAACGAGAACGGAGGAAGCTGAAGAGGGATTCCGTGCGTGCATCCCGG
  GCAGGATCACTGGAGGCCAGAGAAACTATGACTTCATCCCCCACACAGCAGGATGGGAGAGGCAGTCAGA
  GGGGTTCCTCGGACAGCGTGCTTGTGGTTGAGCTTCAGAGAGAGGTAGCCCTGCTGCGAGCCCAGCTGGC
  CTTGGAGCGAAAGCAGAGGCAGGACTACATTGCACGGTCCGTGCAGACCAGCCGTGAGCTAGCAGGCCTG
  CATCACAGCCTGTCTCACTCCCTCCTCACCGTGGCCCAGGCCCCTGAGGCCACTGTTCTGGAGGCTGAGA
  CCCGAAAGCTGGATGAGTCTCTGAATCAGAGTCTGACATCCCCAGGGCCATGTTTGCTACATCCCAGTCT
  GGACACTACTCAAAATACCCACAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216165 representing NM_001130000
  Red=Cloning site Green=Tags(s)
  
  METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQ
  DLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKL
  TVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHAR
  LSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAEL
  QDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQA
  LASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFL
  QQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELL
  QKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSES
  LSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEAL
  ALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAEL
  QAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAV
  ERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAE
  RTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELE
  TKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQ
  KLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEE
  KVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVEL
  LRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQ
  AQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQK
  LHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRD
  LHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSA
  LKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAA
  LELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQ
  LLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDL
  EERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERI
  QVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKE
  VECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEE
  AQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEV
  EGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRA
  ENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEE
  ALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLE
  SLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDL
  ALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGS
  QSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASR
  AGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGL
  HHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130000
• ORF Size: 7305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130000.1, NP_001123472.1
• RefSeq Size: 7977
• RefSeq ORF: 7308
• Locus ID: 16328
• MW: 279 kDa