Chpf (NM_001001566) Mouse Tagged ORF Clone

CAT#: MR216423

Chpf (Myc-DDK-tagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chpf
• Synonyms: 1700028N03Rik; AI414328; D1Bwg1363e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216423 representing NM_001001566
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGTCGCTGCTGCTGTCCGTGCTGCGGCCCGCGGGGCCCGTGGCCGTGGGCATCTCTCTGGGCT
  TCACCCTGAGCCTGCTCAGCGTCACCTGGGTGGAGGAGCCTTGCGGACCCGGGCCGCCCCAACCCGGAGA
  CTCTGAGCTGCCGCCGCGCGGCAACACCAACGCGGCGCGCCGGCCCAACTCGGTGCAGCCCGGATCCGAG
  CGCGAGAGGCCCGGGGCCGGCGCAGGCACCGGTGAGAGCTGGGAGCCTCGTGTCTTGCCCTACCATCCCG
  CGCAGCCAGGCCAGGCCACCAAGAAGGCCGTCAGAACTCGGTATATCAGCACGGAGCTGGGCATCAGGCA
  GAAGCTTCTGGTGGCAGTGCTGACCTCACAAGCCACGTTGCCTACACTGGGTGTGGCTGTAAACCGAACT
  CTGGGACACCGACTGGAGCATGTAGTGTTCCTGACCGGTGCGAGGGGCCGCCGGACACCTTCAGGCATGG
  CGGTGGTGGCACTGGGCGAAGAGAGGCCCATCGGACACCTGCACCTGGCGCTGCGCCACCTGCTGGAGCA
  ACACGGCGATGACTTTGACTGGTTTTTCCTAGTGCCTGATGCCACCTATACTGAAGCGCATGGACTGGAC
  CGCCTAGCTGGCCACCTCAGCCTTGCTTCAGCAACCCATCTCTATCTTGGCCGGCCGCAGGACTTCATCG
  GTGGAGATACTACCCCAGGCCGCTACTGCCACGGGGGCTTTGGAGTCTTGCTCTCTCGCACACTGCTACA
  GCAACTGCGCCCCCACCTGGAAAGCTGCCGCAACGACATCGTCAGTGCTCGCCCGGATGAGTGGTTGGGC
  CGCTGCATCCTTGATGCCACAGGCGTGGGCTGTACTGGTGACCACGAGGGAATGCACTACAACTACCTGG
  AACTGAGCCCCGGGGAGCCTGTGCAGGAGGGGGACCCTCGTTTCCGCAGCGCCTTGACAGCCCATCCCGT
  GCGTGACCCTGTGCACATGTACCAGCTGCACAAAGCTTTTGCCCGCGCTGAGCTGGACCGCACGTACCAG
  GAGATTCAAGAATTGCAGTGGGAGATCCAGAATACCAGCCGACTGGCTGCTGATGGGGAGAGAGCCTCTG
  CCTGGCCAGTGGGCATCCCAGCACCGTCTCGCCCTGCCTCACGCTTTGAGGTTCTGCGCTGGGACTACTT
  CACAGAACAATACGCGTTCTCCTGCGCCGATGGCTCTCCCCGCTGCCCGTTGCGTGGGGCCGACCAGGCT
  GATGTGGCTGACGTCCTGGGGACAGCCTTAGAGGAGCTCAACCGCCGTTACCAGCCAGCGCTGCAGCTCC
  AGAAGCAACAGCTGGTGAACGGCTACCGGCGTTTTGATCCAGCCCGAGGCATGGAGTACACACTAGACCT
  GCAGCTGGAAGCGCTGACACCCCAGGGTGGCCGCTGGCCCCTCACCCGCAGGGTGCAGCTCCTTCGGCCC
  TTGAGCCGAGTGGAGATCTTGCCTGTACCCTATGTCACCGAGGCTTCTCGGCTCACTGTGCTACTGCCGC
  TGGCTGCAGCGGAACGAGACCTGGCTTCTGGCTTCTTAGAAGCCTTTGCCACTGCAGCCCTGGAACCTGG
  TGATGCAGCAGCCTTGACCCTGCTGCTGCTGTATGAGCCACGCCAGGCCCAGCGGGCAGCCCACTCAGAC
  GTCTTCGCACCTGTCAAGGCCCACGTGGCAGAGCTAGAGCGGCGTTTCCCTGGTGCCCGGGTGCCCTGGC
  TCAGTGTGCAGACAGCAGCGCCCTCTCCACTGCGTCTCATGGATCTGCTGTCCAAGAAGCACCCACTAGA
  CACTCTGTTCCTGCTGGCCGGGCCAGACACGGTACTCACACCTGATTTCCTGAACCGCTGCCGCATGCAT
  GCCATCTCTGGCTGGCAGGCCTTCTTCCCCATGCACTTCCAGGCCTTCCACCCTGCTGTGGCTCCACCTC
  AGGGCCCTGGGCCACCAGAGCTGGGCCGTGACACCGGTCACTTTGATCGCCAGGCTGCCAGTGAGGCATG
  CTTCTACAACTCCGACTATGTGGCGGCCCGTGGCCGGCTGGTGGCGGCCTCGGAGCAGGAGGAGGAGCTG
  CTGGAGAGCCTGGATGTGTACGAGTTGTTTCTGCGCTTCTCCAACTTGCACGTGCTGAGAGCAGTAGAGC
  CAGCCTTGCTGCAGCGCTACCGGGCCCAGCCGTGCAGTGCACGGCTCAGTGAAGACCTTTACCACCGCTG
  TCGCCAGAGCGTACTTGAGGGCCTTGGCTCCCGCACCCAGCTTGCCATGCTGCTCTTTGAGCAGGAACAG
  GGGAACAGCACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216423 representing NM_001001566
  Red=Cloning site Green=Tags(s)
  
  MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGSE
  RERPGAGAGTGESWEPRVLPYHPAQPGQATKKAVRTRYISTELGIRQKLLVAVLTSQATLPTLGVAVNRT
  LGHRLEHVVFLTGARGRRTPSGMAVVALGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDATYTEAHGLD
  RLAGHLSLASATHLYLGRPQDFIGGDTTPGRYCHGGFGVLLSRTLLQQLRPHLESCRNDIVSARPDEWLG
  RCILDATGVGCTGDHEGMHYNYLELSPGEPVQEGDPRFRSALTAHPVRDPVHMYQLHKAFARAELDRTYQ
  EIQELQWEIQNTSRLAADGERASAWPVGIPAPSRPASRFEVLRWDYFTEQYAFSCADGSPRCPLRGADQA
  DVADVLGTALEELNRRYQPALQLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRWPLTRRVQLLRP
  LSRVEILPVPYVTEASRLTVLLPLAAAERDLASGFLEAFATAALEPGDAAALTLLLLYEPRQAQRAAHSD
  VFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMH
  AISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGHFDRQAASEACFYNSDYVAARGRLVAASEQEEEL
  LESLDVYELFLRFSNLHVLRAVEPALLQRYRAQPCSARLSEDLYHRCRQSVLEGLGSRTQLAMLLFEQEQ
  GNST
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001566
• ORF Size: 2322 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001566.1, NM_001001566.2, NM_001001566.3, NP_001001566.1
• RefSeq Size: 3107 bp
• RefSeq ORF: 2325 bp
• Locus ID: 74241
• Cytogenetics: 1 39.14 cM
• MW: 85.5 kDa