Pde4a (NM_183408) Mouse Tagged ORF Clone

CAT#: MR216607

Pde4a (Myc-DDK-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4a
• Synonyms: D9Ertd60e; Dpde2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216607 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTCCGGCCGCCCCCTCGGAGAGGAGCCTGTCTCTCTCTCTTCCCGGGCCCCGAGAGGGCCAGG
  CCACCCTGAAGCCGCCCCCCCAGCACCTGTGGCGGCAGCCGAGGACCCCGATCCGCATCCAGCAGCGCGG
  CTACTCGGACAGTGCCGAGCGCTCAGAGCCAGAGCGCTCGCCGCACCGGCCCATAGAGCGCGCCGACGCC
  GTGGACACTGGCGACCGGCCAGGCCTGCGGACTACCCGCATGTCCTGGCCCTCGTCCTTCCACGGCACCG
  GTACCGGCGGAGGCAGCAGTAGGCGCTTGGAGGCAGAAAATGGGCCAACACCGTCCCCTGGCCGCAGCCC
  CCTGGACTCGCAGGCGAGCCCGGGGCTTATGCTGCATGCCGGGGCAGCCACCAGCCAGCGCCGAGAATCC
  TTCCTCTATCGCTCAGACAGCGACTATGACATGTCACCGAAGACTATGTCCAGGAACTCATCCGTCGCCA
  GCGAAGCGCATGGTGAAGACCTCATTGTGACCCCGTTTGCCCAGGTGCTGGCCAGTCTCCGCAACGTTCG
  AAGCAACTTCTCACTCCTAACCAATGTGCCCATCCCCAGCAACAAGAGGTCCCCGCTGGGCGGGCCACCC
  TCTGTCTGCAAGGCCACACTGTCAGAGGAGACATGCCAGCAGCTGGCCCGGGAGACCCTGGAAGAGCTGG
  ATTGGTGCCTGGAGCAGCTGGAGACCATGCAGACCTACCGCTCTGTCAGCGAGATGGCCTCACACAAGTT
  TAAAAGGATGCTGAACCGGGAACTCACACACCTGTCGGAAATGAGCAGGTCAGGAAACCAGGTCTCAGAG
  TACATTTCCAACACGTTCCTAGACAAGCAGCACGAAGTGGAGATCCCATCGCCCACGCCGCGACAGAGAC
  CCTTCCAGCAGCCCCCACCAGCAGCAGTGCAGCAGGCCCAGCCCATGTCTCAGATCACAGGGCTGAAAAA
  GCTGGTACACACCGGAAGCTTGAACATCAATGTCCCACGATTTGGAGTCAAGACAGATCAGGAGGACCTC
  TTAGCACAAGAACTGGAGAACTTGAGCAAATGGGGCCTGAACATCTTTTGTGTGTCGGAGTACGCTGGAG
  GCCGTTCACTCAGCTGTATCATGTATACGATATTCCAGGAGCGGGACTTACTGAAGAAATTCCACATCCC
  CGTGGACACCATGATGACATACATGCTGACGCTGGAGGACCACTACCACGCTGACGTGGCCTACCACAAC
  AGCCTGCACGCAGCCGATGTGCTACAGTCCACACATGTGCTGCTGGCCACGCCTGCACTGGATGCCGTGT
  TCACAGACCTGGAGATTCTTGCTGCTCTCTTCGCTGCTGCCATCCATGACGTGGACCACCCTGGCGTCTC
  CAACCAGTTCCTAATCAACACCAATTCGGAGCTGGCATTGATGTACAACGATGAGTCTGTGCTTGAGAAC
  CACCACCTGGCTGTGGGATTCAAGCTGCTGCAAGAAGAGAACTGCGACATCTTCCAGAACCTCAGCAAGC
  GCCAGCGGCAGAGCCTGCGCAAGATGGTCATCGACATGGTGCTGGCCACAGACATGTCCAAGCACATGAC
  CCTTCTGGCTGACCTGAAGACTATGGTAGAGACGAAGAAAGTGACCAGCTCTGGAGTTCTCTTGCTGGAC
  AACTACTCTGACCGTATCCAGGTCCTCAGGAACATGGTGCACTGTGCAGACCTCAGCAATCCCACCAAGC
  CCCTGGAGCTGTACCGACAATGGACTGATCGCATCATGGCTGAGTTCTTCCAGCAGGGTGACCGAGAACG
  GGAACGCGGAATGGAGATTAGCCCCATGTGTGACAAGCACACAGCCTCTGTGGAGAAGTCTCAGGTGGGC
  TTCATCGACTATATTGTTCACCCACTGTGGGAGACGTGGGCCGATCTCGTCCACCCCGATGCCCAAGACA
  TCCTGGACACGCTGGAAGACAACCGGGATTGGTATCACAGTGCCATCCGCCAGAGTCCCTCCCCAACCCT
  GGAAGAGGAGCCAGGAGTGCTCAGCGATCCGGCCCTGCCTGACAAGTTCCAATTTGAGCTCACCTTGGAG
  GAAGAGGATGAAGAGGATTCATTGGAGGTTCCAGGATTGCCTTGCACTGAGGAAACTCTCCTGGCTCCAC
  ATGATACCAGAGCTCAAGCCATGGAACAGTCAAAGGTCAAAGGCCAGAGCCCTGCTGTTGTGGAGGTAGC
  AGAGAGTTTGAAGCAGGAGACAGCCTCAGCACATGGCGCTCCTGAGGAGTCCGCGGAGGCTGTGGGCCAT
  TCCTTCAGCCTTGAAACCTCTATTCTGCCTGACTTGAGGACCCTGTCCCCCTCAGAGGAGGCCCAGGGCC
  TCCTGGGCCTCCCCTCCATGGCGGCAGAGGTGGAGGCCCCAAGAGACCATCTGGCTGCCATGAGGGCTTG
  TTCTGCCTGCTCGGGGACATCAGGAGACAATTCTGCCGTCATCTCTGCTCCAGGCAGGTGGGGGTCAGGT
  GGAGACCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216607 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERSEPERSPHRPIERADA
  VDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLMLHAGAATSQRRES
  FLYRSDSDYDMSPKTMSRNSSVASEAHGEDLIVTPFAQVLASLRNVRSNFSLLTNVPIPSNKRSPLGGPP
  SVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSE
  YISNTFLDKQHEVEIPSPTPRQRPFQQPPPAAVQQAQPMSQITGLKKLVHTGSLNINVPRFGVKTDQEDL
  LAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMTYMLTLEDHYHADVAYHN
  SLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLEN
  HHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLD
  NYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVG
  FIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPTLEEEPGVLSDPALPDKFQFELTLE
  EEDEEDSLEVPGLPCTEETLLAPHDTRAQAMEQSKVKGQSPAVVEVAESLKQETASAHGAPEESAEAVGH
  SFSLETSILPDLRTLSPSEEAQGLLGLPSMAAEVEAPRDHLAAMRACSACSGTSGDNSAVISAPGRWGSG
  GDPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183408
• ORF Size: 2535 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_183408.1, NM_183408.2, NM_183408.3, NP_899668.1
• RefSeq Size: 4658
• RefSeq ORF: 2535
• Locus ID: 18577
• MW: 93.6 kDa