Polg2 (NM_015810) Mouse Tagged ORF Clone

CAT#: MR216884

Polg2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), gamma 2, accessory subunit (Polg2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polg2
• Synonyms: mtPolB; p55; polG-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216884 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGCGGTGGCGGCGCCAGGGCCTGCCGCAGGGCCTGCAGGTGCTGGCTGTCCGGGTACGCGGGTC
  CCGCAGATGGGACGCAGCAGCCTGACGCCCCCGAGCATGCGGTGGCCCGCGAGGCGCTGGTAGACCTCTG
  CCGGAGGAGGCACTTTCTCAGCGGGACGCCGCAGCAGCTCAGCACGGCTGCTCTGCTGAGCGGGTGCCAC
  GCGCGCTTTGGACCTTTGGGTGTAGAGTTACGAAAGAACCTAGCCTCACAGTGGTGGTCCTCTATGGTGG
  TGTTCAGGGAGCAGGTCTTCGCGGTGGACTCCCTCCACCAGGAGCCGGGCTCTTCGCAGCCGAGGGACAG
  TGCCTTCAGGTTAGTCTCTCCAGAAAGTATACGCGAAATCTTGCAAGACAGAGAGCCGAGTAAGGAACAG
  CTAGTGGCATTTCTTGAGAACTTATTAAAAACTTCTGGGAAACTACGGGCGACTCTTCTTCACGGTGCCT
  TGGAGCACTATGTTAATTGCCTGGATCTGGTAAACAGAAAGCTACCTTTCGGTCTTGCTCAGATTGGAGT
  CTGTTTCCATCCTGTTTCGAACAGCAATCAGACACCCAGCAGTGTTACAAGAGTTGGTGAAAAGACGGAA
  GCTTCCTTGGTATGGTTTACTCCCACAAGGACTTCGAGCCAGTGGCTTGATTTCTGGTTGCGTCATCGGC
  TTCTGTGGTGGAGAAAGTTTGCTATGAGTCCCTCTAACTTCAGCAGCGCTGATTGTCAGGATGAATTGGG
  ACGCAAGGGAAGCAAACTTTACTACAGCTTCCCCTGGGGAAAGGAGCCAATAGAAACCCTGTGGAACCTA
  GGAGATCAAGAACTCCTACACACATATCCTGGAAATGTGTCTACGATACAGGGTCGAGATGGGCGGAAAA
  ACGTGGTTCCTTGCGTTCTGTCTGTAAGTGGGGACGTAGACCTGGGCACGCTGGCTTACCTCTACGATTC
  CTTCCAGCTGGCAGAGAACTCCTTTGCCAGGAAGAAGAGCCTCCAGAGAAAGGTGCTTAAACTTCACCCT
  TGTTTAGCCCCTATTAAGGTCGCTTTGGATGTGGGGAAAGGCCCAACGGTAGAACTCCGACAGGTTTGTC
  AAGGGCTACTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGGCTATTCGGAAACTGTCCATTC
  CTCATTAGAGCAACTCCATTCAAAGTACGATGAGATGAGTGTCCTCTTCTCAGTTCTGGTCACTGAGACT
  ACGCTGGAAAATGGGCTAATCCAGCTGCGGAGCAGAGACACCACGATGAAGGAAATGATGCACATATCCA
  AGCTGAGAGACTTCCTGGTCAAGTACCTAGCGTCAGCTAGCAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216884 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRCGGGARACRRACRCWLSGYAGPADGTQQPDAPEHAVAREALVDLCRRRHFLSGTPQQLSTAALLSGCH
  ARFGPLGVELRKNLASQWWSSMVVFREQVFAVDSLHQEPGSSQPRDSAFRLVSPESIREILQDREPSKEQ
  LVAFLENLLKTSGKLRATLLHGALEHYVNCLDLVNRKLPFGLAQIGVCFHPVSNSNQTPSSVTRVGEKTE
  ASLVWFTPTRTSSQWLDFWLRHRLLWWRKFAMSPSNFSSADCQDELGRKGSKLYYSFPWGKEPIETLWNL
  GDQELLHTYPGNVSTIQGRDGRKNVVPCVLSVSGDVDLGTLAYLYDSFQLAENSFARKKSLQRKVLKLHP
  CLAPIKVALDVGKGPTVELRQVCQGLLNELLENGISVWPGYSETVHSSLEQLHSKYDEMSVLFSVLVTET
  TLENGLIQLRSRDTTMKEMMHISKLRDFLVKYLASASNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015810
• ORF Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015810.1, NM_015810.2, NP_056625.2
• RefSeq Size: 1585
• RefSeq ORF: 1380
• Locus ID: 50776
• MW: 51.4 kDa
• Gene Summary: Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA. [UniProtKB/Swiss-Prot Function]