Cars2 (NM_024248) Mouse Tagged ORF Clone

CAT#: MR216919

Cars2 (Myc-DDK-tagged) - Mouse cysteinyl-tRNA synthetase 2 (mitochondrial)(putative) (Cars2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cars2
• Synonyms: 2310051N18Rik; 2410044A07Rik; cysRS; D530030H10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216919 representing NM_024248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGGCGCGCGGGCCTGGGCCCGGCGCTCTGCTGCTCCGGGCCGCGCTGGGTCTCGGGCGGCGCG
  GGCGGTCATGGCAACGGCCGCAAGGCCAGGACACCGGCGTGCAGGTGCATAACAGCCTCACTGGCAGGAA
  GGAGCCGCTGATAGTGGCCCGCTCGGACGCAGTCTCCTGGTATAGCTGTGGACCGACTGTGTACGACCAT
  GCACATCTTGGCCATGCTTGCTCCTATGTTAGATTTGACATTATCCGGAGGATCCTGACCAGAGTGTTTG
  GATGCAATGTGGTCATGGCGATGAGCATTACCGACGTGGATGACAAAATAATCAAGAGAGCTAACGAGAT
  GAACGTGACGCCCGCTTCTCTCGCCAGTCTTTTTGAGGAAGAATTTAAACAAGACATGGCAGCCCTGAAG
  GTTCTGCCGCCAACTGTGTATTTGAGAGTCACTGAGAACATCCCTCAGATCATTGCTTTCATCGAGGGCA
  TCATTGCTCACGGCCATGCATACTCGACAGCTACAGGCAGCGTCTACTTCGATCTGCACGCCCGAGGGGA
  CAAGTATGGCAAGCTGGTCAACACGGTTCCCAGTGCCACTGCAGAGCCAGCAGGTGACTCTGACAAGCGG
  CACAGCAGCGACTTCGCCCTGTGGAAGGCAGCCAAACCTCAGGAGGTGTTCTGGGCTTCGCCGTGGGGAG
  ACGGACGGCCTGGATGGCACATCGAGTGCTCTACCATGGCCAGTGAGGTGTTCGGAAGCCACCTGGACAT
  CCACACCGGCGGCATAGACTTGGCTTTCCCACATCACGAAAATGAAATCGCGCAGAGTGAAGTCTTCCAC
  CAGTGTCAGCAGTGGGGAAATTACTTCCTACATTCTGGTCATTTGCATGTGAAAGGCACAGAAGAAAAGA
  TGTCCAAATCCCTAAAAAACTATATCACCATTAAGGACTTCCTGAAGACCTTCTCCCCTGACGTCTTCCG
  GCTTTTCTGCTTGCGTACCAACTATAGGTCAGCCATTGAATACAGTGACAGTACCTTGGTGGAAGCCAAG
  CACCTCCTGCTGGGGCTGGCCTCTTTTGTGGAGGATGCACGAGCCTACGTAAAGGGGCAGCTGACCTGTG
  GCCCTGTTGAGGAGGATGTGCTATGGGAGAGGCTGACTAGCACCAAGAAGGCGGTGAAAGCTGCTCTAGC
  CAATGACTTTGACACACCGAGGGCAGTGAACACCATCCTGGACCTTGTGCACCATGCAAACAGACAGCTC
  AGGGCGGTCTCCAAGGAAGCTAGTGGCCCAAGGAGTCCCACTGTGTTTGGGGCCATCATTTCCTACGTTG
  AGCAGTTTTTCGAGACTGTTGGGATTTCTCTGGCAAATCGACAGTGTGTTTCAGGAGACAGCAGCACAGT
  AACGTTGCGCTGTGTGGTAGATGAACTCGTGCGCTTCAGGCTGAAGGTCCGCCAGTATGCACTGGACACA
  CCCGGGGCTGCTGGGGAGGCTCGGAAACGGCAGCTCCAGGAAAGGCAGCCCTTGCTGGAGGCATGTGACA
  CACTCCGACAGGACCTCGTCACCCATGGCATCAATGTTAAGGACAGAGGCAGTGCAGCCTCCACATGGGA
  ACTGCTGGATCCAAGGACAAGACACCAGAAACCTGGGGACCGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216919 representing NM_024248
  Red=Cloning site Green=Tags(s)
  
  MLRARGPGPGALLLRAALGLGRRGRSWQRPQGQDTGVQVHNSLTGRKEPLIVARSDAVSWYSCGPTVYDH
  AHLGHACSYVRFDIIRRILTRVFGCNVVMAMSITDVDDKIIKRANEMNVTPASLASLFEEEFKQDMAALK
  VLPPTVYLRVTENIPQIIAFIEGIIAHGHAYSTATGSVYFDLHARGDKYGKLVNTVPSATAEPAGDSDKR
  HSSDFALWKAAKPQEVFWASPWGDGRPGWHIECSTMASEVFGSHLDIHTGGIDLAFPHHENEIAQSEVFH
  QCQQWGNYFLHSGHLHVKGTEEKMSKSLKNYITIKDFLKTFSPDVFRLFCLRTNYRSAIEYSDSTLVEAK
  HLLLGLASFVEDARAYVKGQLTCGPVEEDVLWERLTSTKKAVKAALANDFDTPRAVNTILDLVHHANRQL
  RAVSKEASGPRSPTVFGAIISYVEQFFETVGISLANRQCVSGDSSTVTLRCVVDELVRFRLKVRQYALDT
  PGAAGEARKRQLQERQPLLEACDTLRQDLVTHGINVKDRGSAASTWELLDPRTRHQKPGDRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024248
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024248.1, NP_077210.1
• RefSeq Size: 1867
• RefSeq ORF: 1659
• Locus ID: 71941
• MW: 61.3 kDa