Frk (NM_010237) Mouse Tagged ORF Clone

CAT#: MR216926

Frk (Myc-DDK-tagged) - Mouse fyn-related kinase (Frk), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Frk
• Synonyms: BSK; BSK/IYK; C85044; GTK; RAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216926 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCGTCTGTGTGAGACTCTGGGCATACCTGCAGCCTTTTCTCCCGTGCTGGTCTCAAGAGGCAG
  ACAAGTCAGTAGTAATTGAGAATCCAGGGGCCTTCTGTCCCCCAGAGGCTCCCAGGTCACAAGAGCCCGA
  GAGAAGCCATGGCCAGTATTTTGTGGCTCTGTTTGATTACCAAGCACGTACTGCAGAGGACCTGAGCTTC
  CGTGCCGGCGACAAACTCCAAGTCTTGGACACTTCGCATGAGGGCTGGTGGTTGGCCAGACATTTGGAGA
  AGAAGGGAACCGGCTTAGGTCAGCAGCTACAGGGCTACATTCCTTCCAATTACGTGGCGGAGGACCGGAG
  TCTCCAGGCAGAGCCGTGGTTTTTTGGAGCAATCAAAAGAGCAGATGCAGAAAAACAACTTCTGTATTCA
  GAAAACCAGACGGGCGCCTTTCTAATCAGAGAGAGTGAGAGCCAGAAGGGTGACTTTTCCCTCTCAGTTT
  TAGATGAAGGTGTTGTAAAACACTACAGAATAAGAAGGTTGGATGAAGGTGGCTTCTTCCTCACCAGGAG
  GAAAGTCTTTTCAACCCTGAATGAATTCGTGAACTACTACACCACAACAAGTGACGGGCTGTGTGTCAAG
  CTGGAGAAGCCATGCTTAAAGATCCAGGTACCAACCCCTTTTGATTTGTCATATAAAACTGCAGACCAGT
  GGGAGATAGACCGCAACTCCATACAGCTTTTGAAGCGACTGGGATCTGGTCAGTTTGGAGAAGTTTGGGA
  AGGTCTGTGGAATAATACCACTCCAGTGGCCGTAAAAACGTTAAAACCAGGTTCAATGGATCCAAATGAC
  TTCCTGAGGGAGGCACAGATAATGAAGAGCCTAAGACACCCAAAACTCATCCAGCTCTATGCTGTTTGCA
  CTTTAGAAGATCCCATTTATATTATTACAGAGTTGATGAGACATGGAAGCCTGCAAGAATATCTCCAAAA
  TGATGGTGGGTCAAAAATCCATTTGATTCAACAGGTAGACATGGCGGCACAGGTGGCTTCTGGAATGGCC
  TATCTTGAGTCGCAGAACTATATTCACAGAGATCTGGCTGCAAGAAATGTCCTTGTTGGTGAACATAATA
  TCTACAAAGTAGCAGATTTTGGACTTGCAAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAA
  ACACGAAATAAAGCTGCCAGTGAAGTGGACTGCACCCGAAGCCATTCGTACTAATAAATTCAGCATTAAG
  TCTGATGTGTGGTCTTTTGGAATCCTGCTCTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTA
  TGACAGGTGCTCAAGTAATTCAAATGTTGAGTCAAAACTACAGACTTCCACAGCCATCTAACTGCCCACA
  GCAATTCTACAGCATCATGCTAGAGTGCTGGAATGTTGAGCCTAAGCAACGGCCAACATTTGAGACCCTG
  CATTGGAAACTTGAAGACTACTTTGAAACAGACTGTTCCTATTCAGATACAAATAACTTCATAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216926 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSVCVRLWAYLQPFLPCWSQEADKSVVIENPGAFCPPEAPRSQEPERSHGQYFVALFDYQARTAEDLSF
  RAGDKLQVLDTSHEGWWLARHLEKKGTGLGQQLQGYIPSNYVAEDRSLQAEPWFFGAIKRADAEKQLLYS
  ENQTGAFLIRESESQKGDFSLSVLDEGVVKHYRIRRLDEGGFFLTRRKVFSTLNEFVNYYTTTSDGLCVK
  LEKPCLKIQVPTPFDLSYKTADQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPND
  FLREAQIMKSLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDGGSKIHLIQQVDMAAQVASGMA
  YLESQNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESKHEIKLPVKWTAPEAIRTNKFSIK
  SDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLSQNYRLPQPSNCPQQFYSIMLECWNVEPKQRPTFETL
  HWKLEDYFETDCSYSDTNNFIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010237
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010237.1, NM_010237.2, NM_010237.3, NP_034367.2
• RefSeq Size: 4669 bp
• RefSeq ORF: 1539 bp
• Locus ID: 14302
• MW: 58.8 kDa