Spire1 (NM_176832) Mouse Tagged ORF Clone

CAT#: MR217344

Spire1 (Myc-DDK-tagged) - Mouse spire homolog 1 (Drosophila) (Spire1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spire1
• Synonyms: 6030430B19Rik; AI415299; AU022898; AW550622; Spir-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217344 representing NM_176832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGCATACATTTCTGACCAAAATTAAGAGTGCAAAGGAGAACCTTAAGAAGATTCAAGAAATGG
  AAAAGGGTGATGAATCTAGCACAGATCTGGAGGACCTGAAAAATGCAGACTGGGCCCGGTTCTGGGTACA
  AGTGATGAGGGATTTGCGAAATGGGGTAAAACTTAAGAAAGTCCAGCAGCGGCAGTACAACCCTCTGCCC
  ATTGAGTACCAACTGACCCCTTATGAGATGCTCATGGACGACATTCGGTGCAAAAGATACACCTTGAGAA
  AAGTAATGGTAAATGGTGACGTCCCCCCTCGGTTGAAAAAGAGTGCTCATGAGGTCATCCTTGACTTTAT
  CAGATCAAGACCCCCTTTAAATCCAGTTTCAGCCAGAAAACTGAAACCAACCCCACCACGGCCACGGAGC
  CTCCATGAAAGAATATTAGAAGAAATTAAAGCAGAAAGAAAGCTTCGACCTGTGTCACCAGAAGAAATTA
  GACGGAGCAGACTAGATGTCACTACGCCAGAATCTCCAAAGAATGTTGGAGAATCATCTATGGTGAATGG
  AGGCTTAACATCTCAAACAAAAGAAAATGGGCTGAGCGCTGCCCAGCAGGGGTCTGCGCAGAGGAAGAGG
  CTCCTCAAAGCCCCAACCCTGGCTGAACTGGACAGCTCTGACTCTGAGGAGGAAAAAAGTCTGCACAAGT
  CAACCAGCAGCAGCAGTGCGTCTCCCTCCCTCTATGAGGATCCAGTACTGGAGGCCATGTGCTCCAGGAA
  GAAGCCTCCAAAATTCTTGCCCATCTCATCAACACCCCAGCCAGAGAGAAGGCAGCCACCGCAGAGGCGC
  CACTCCATTGAGAAGGAAACACCTACGAACGTGAGGCAGTTTCTGCCACCATCCAGGCAGAGCTCACGGT
  CTCTTGAGGAATTCTGCTACCCAGTGGAGTGCCTCGCTCTCACTGTGGAGGAAGTGATGCACATCCGTCA
  GGTCCTGGTGAAAGCAGAGCTGGAAAAGTACCAACAGTATAAGGATGTCTACACTGCCCTGAAAAAAGGA
  AAGCTCTGCTTCTGTTGCCGAACCCGGAGGTTTTCCTTCTTCACCTGGTCTTACACCTGTCAGTTCTGTA
  AGAGGCCCGTGTGCTCACAGTGTTGCAAAAAGATGCGGCTGCCTTCCAAGCCATACTCTACTCTACCTAT
  CTTCTCTTTGGGACCTTCTGCCTTGCAAAGGGGGGAAAGCTGTTCGAGGTCAGAAAAACCCTCCACTAGT
  CACCATCGGCCTCTTCGAAGTATTGCCAGGTTCTCCACAAAATCCAGGTCTGTGGACAAGTCCGATGAGG
  AGCTCCAGTTTCCCAAAGAGCTCATGGAGGACTGGAGCACGATGGAGGTGTGTGTGGACTGCAAAAAGTT
  CATCTCCGAGATCATCAGCTCAAGCCGGCGCAGCCTGGTGCTGGCTAACAAAAGAGCCAGGTTGAAAAGG
  AAGACACAGTCTTTCTACATGTCCTCGGCGGGCCCCTCGGAGTACTGCCCTTCAGAACGGACTATCAACG
  AGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217344 representing NM_176832
  Red=Cloning site Green=Tags(s)
  
  MELHTFLTKIKSAKENLKKIQEMEKGDESSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLP
  IEYQLTPYEMLMDDIRCKRYTLRKVMVNGDVPPRLKKSAHEVILDFIRSRPPLNPVSARKLKPTPPRPRS
  LHERILEEIKAERKLRPVSPEEIRRSRLDVTTPESPKNVGESSMVNGGLTSQTKENGLSAAQQGSAQRKR
  LLKAPTLAELDSSDSEEEKSLHKSTSSSSASPSLYEDPVLEAMCSRKKPPKFLPISSTPQPERRQPPQRR
  HSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKG
  KLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTS
  HHRPLRSIARFSTKSRSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKR
  KTQSFYMSSAGPSEYCPSERTINEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176832
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_176832.1, NM_176832.2, NP_789802.1
• RefSeq Size: 4761
• RefSeq ORF: 1548
• Locus ID: 68166
• MW: 59.8 kDa