Dars (NM_145507) Mouse Tagged ORF Clone

CAT#: MR217423

Dars (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dars
• Synonyms: 5730439G15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217423 representing NM_145507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCACCAACGCCAGCCGTAAGGGTCAGGAGAAGCCGCGGGAGATCGTGGACGCGGCGGAAGATT
  ATGCTAAAGAGAGATATGGGATATCTTCTATGATACAATCACAAGAAAAGCCAGATAGAGTTTTGGTTCG
  AGTTAAGGACCTGACAGTTCAAAAAGCTGATGATGTTGTTTGGGTCCGTGCAAGAGTTCATACAAGCAGA
  GCAAAAGACTGGGAACCTACCGACCGTTTGTTCTTTCTCTTTTTTAACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217423 representing NM_145507
  Red=Cloning site Green=Tags(s)
  
  MPSTNASRKGQEKPREIVDAAEDYAKERYGISSMIQSQEKPDRVLVRVKDLTVQKADDVVWVRARVHTSR
  AKDWEPTDRLFFLFFNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145507
• ORF Size: 261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145507.2, NP_663482.2
• RefSeq Size: 702
• RefSeq ORF: 264
• Locus ID: 226414
• MW: 10.7 kDa
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. [UniProtKB/Swiss-Prot Function]