Idh1 (NM_010497) Mouse Tagged ORF Clone

CAT#: MR217871

Idh1 (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 1 (NADP+), soluble (Idh1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Idh1
• Synonyms: AI314845; AI788952; E030024J03Rik; Id-1; Idh-1; Idpc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217871 representing NM_010497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGAAAAATCCAAGGAGGTTCTGTGGTGGAGATGCAAGGAGATGAAATGACACGAATCATTTGGG
  AATTGATTAAGGAAAAACTTATTCTTCCCTATGTGGAACTGGATCTGCATAGCTATGATTTAGGCATAGA
  GAATCGTGATGCCACCAATGACCAGGTCACCAAAGATGCTGCAGAGGCTATAAAGAAATACAACGTGGGC
  GTCAAGTGTGCTACCATCACCCCCGATGAGAAGAGGGTTGAAGAATTCAAGTTGAAACAAATGTGGAAAT
  CCCCAAATGGCACCATCCGAAACATTCTGGGTGGCACTGTCTTCAGGGAAGCTATTATCTGCAAAAATAT
  CCCCCGGCTAGTGACAGGCTGGGTAAAACCCATCATCATTGGCCGACATGCATATGGGGACCAATACAGA
  GCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAAAGATGGAACTCAGA
  AGGTGACATACATGGTACATGACTTTGAAGAAGGTGGTGGTGTTGCCATGGGCATGTACAACCAGGATAA
  GTCAATTGAAGACTTTGCACACAGTTCCTTCCAAATGGCTCTGTCCAAGGGCTGGCCTTTGTATCTCAGC
  ACCAAGAACACTATTCTGAAGAAGTATGATGGGCGTTTCAAAGACATCTTCCAGGAGATCTATGACAAGA
  AATACAAGTCCCAGTTTGAAGCTCAGAAGATCTGCTATGAACACAGGCTCATAGATGACATGGTGGCCCA
  AGCTATGAAGTCCGAGGGAGGCTTCATCTGGGCCTGTAAGAATTACGATGGGGATGTGCAGTCAGACTCA
  GTCGCCCAAGGTTATGGCTCCCTTGGCATGATGACCAGTGTGCTGATTTGTCCAGATGGTAAGACGGTAG
  AAGCAGAGGCTGCCCATGGCACTGTCACACGTCACTACCGCATGTACCAGAAAGGGCAAGAGACGTCCAC
  CAACCCCATTGCTTCCATTTTTGCCTGGTCCCGAGGGTTAGCCCACAGAGCAAAGCTTGATAACAATACT
  GAGCTCAGCTTCTTCGCAAAGGCTTTGGAAGACGTCTGCATTGAGACCATTGAGGCTGGCTTTATGACTA
  AGGACTTGGCTGCTTGCATTAAAGGCTTACCCAATGTACAACGTTCTGACTACTTGAATACATTTGAGTT
  TATGGACAAACTTGGAGAAAACTTGAAGGCCAAATTAGCTCAGGCCAAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217871 representing NM_010497
  Red=Cloning site Green=Tags(s)
  
  MSRKIQGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVG
  VKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYR
  ATDFVVPGPGKVEITYTPKDGTQKVTYMVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLS
  TKNTILKKYDGRFKDIFQEIYDKKYKSQFEAQKICYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS
  VAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNT
  ELSFFAKALEDVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010497
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010497.1, NM_010497.2, NM_010497.3, NP_034627.3
• RefSeq Size: 2270
• RefSeq ORF: 1245
• Locus ID: 15926
• MW: 46.7 kDa