Pum1 (NM_001159605) Mouse Tagged ORF Clone

CAT#: MR218109

Pum1 (Myc-DDK-tagged) - Mouse pumilio 1 (Drosophila) (Pum1), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pum1
• Synonyms: AA517475; mKIAA0099; Pumm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218109 representing NM_001159605
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC
  ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA
  GCCACAGCCAGCTGCAAATCAGGCTCTTGCAGCTGGAACACACTCCAGCCCTGTCCCAGGATCCATAGGA
  GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
  GGGGAGGAGGAAGTGGTGGAGGCGGCTATAATACTAGCAAACATCGATGGCCTACAGGGGATAACATTCA
  TGCAGAACACCAGGTACGCTCTATGGATGAATTGAATCATGATTTTCAAGCACTTGCTCTGGAAGGGAGA
  GCTATGGGCGAGGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAACAAGGGTA
  CGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGAGATGTGATGGACAAGACCAATGGTTTGCC
  AGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGAACTCTGCT
  AATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAGCACCAATG
  GTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATGGAACATGT
  GGGCATGGAGCCTCTGCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCTGCAGCAGCAACTGTG
  GGACTTTTTGACTACAATTCTCAACAACAGCTATTCCAAAGACCCAATGCACTTGCTGTTCAGCAGTTGA
  CAGCTGCCCAGCAGCAGCAGTATGCACTTGCAGCAGCCCACCAGCCTCACATCGGTTTAGCTCCCGCTGC
  GTTTGTCCCCAACCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTGGATTGGCT
  GCAGCAGCGACACTCGGCCCAGCTGTGGTCCCTCACCAATATTATGGAGTCACGCCATGGGGTGTCTACC
  CTGCCAGTCTCTTCCAGCAGCAGGCAGCTGCAGCTGCAGCAGCTACAAACTCTGCTACTCAGCAGAGCGC
  TCCGCAGGCCCAGCAGGGACAGCAGCAGGTTCTTCGTGGAGGAGCTAGCCAACGTCCTTTGACCCCAAAT
  CAGAACCAGCAGGGACAACAAACAGATCCCCTTGTAGCAGCTGCAGCAGTGAATTCTGCTCTTGCCTTTG
  GACAAGGATTGGCTGCAGGTATGCCAGGTTATCCAGTCTTGGCACCTGCTGCTTACTATGACCAAACTGG
  TGCCCTTGTGGTGAATGCAGGGGCAAGAAATGGCCTCGGAGCTCCAGTCCGGCTTGTTGCTCCAGCCCCA
  GTCATCATTAGCTCCTCAGCTGCACAAGCAGCTGTTGCAGCGGCTGCAGCGTCAGCAAACGGAGCAGCTG
  GTGGTCTTGCCGGAACTACAAATGGACCATTTCGACCTTTAGGCACCCAGCAGCCCCAGCCCCAGCCTCA
  GCAGCAGCCCAGTAACAATCTGGCTTCCAGCTCTTTCTATGGCAACAACTCTCTGAGCAGTAACTCCCAG
  AGCAGCTCCCTCTTCTCTCAGGGCTCTGCACAGCCTGCCAACACGTCCTTGGGATTCGGAAGCAGCAGTT
  CCCTTGGTGCCACCCTGGGATCGGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAACACTGGCAG
  TGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCTTGGCCCCC
  ATTGGACACAGTTTTTATAGCAGCCTTAGCTATTCCTCCTCTCCTGGACCCGTGGGCATGCCTCTCCCTA
  GTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCAAGCTTAAA
  CCTGGGAGGACTCACAAACGGCAGTGGAAGATACATCTCTGCTGCTCCCGGTGCCGAAGCCAAGTACCGC
  AGTGCAAGCAGTGCCTCCAGCCTTTTCAGCCCCAGCAGCACCCTTTTTTCTTCCTCTCGTTTGCGATATG
  GAATGTCTGATGTTATGCCCTCTGGCAGGAGCAGACTTCTGGAAGATTTTCGAAACAACCGGTACCCTAA
  TTTACAACTGCGGGAGATTGCTGGGCATATAATGGAATTTTCTCAAGACCAGCATGGCTCTAGATTCATT
  CAGCTGAAACTAGAGCGTGCCACAGCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCAGGCTGCGT
  ACCAACTTATGGTGGATGTGTTTGGAAATTACGTCATTCAGAAGTTTTTTGAATTTGGCAGTCATGAACA
  GAAGCTGGCTCTGGCTGAACGGATTCGAGGCCATGTCCTATCATTGGCACTGCAGATGTACGGCTGCCGA
  GTCATCCAGAAAGCTTTGGAGTTTATTCCCTCAGACCAGCAGGTAATTAACGAGATGGTGCGGGAGCTAG
  ATGGCCATGTCCTGAAGTGTGTGAAAGACCAGAACGGCAACCATGTGGTTCAGAAGTGCATTGAGTGTGT
  ACAGCCCCAGTCTTTGCAGTTTATCATCGATGCATTCAAGGGGCAGGTGTTTGCTTTGTCTACGCACCCG
  TACGGCTGTAGGGTGATCCAGAGGATCCTAGAGCACTGTCTCCCTGACCAGACCCTCCCCATCCTGGAGG
  AGCTTCACCAGCACACAGAGCAGCTTGTGCAGGATCAGTATGGAAATTATGTAATCCAACATGTACTGGA
  GCATGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAGATCCGAGGCAATGTACTTGTATTGAGTCAG
  CACAAGTTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACAGAGCGTGCTGTGCTCA
  TTGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGATCAGTATGC
  TAACTATGTGGTCCAGAAGATGATCGATGTGGCTGAGCCGGGTCAGCGGAAGATTGTCATGCACAAGATC
  CGGCCACACATTGCGACGCTTCGAAAGTACACCTATGGCAAGCACATCCTGGCCAAGCTCGAGAAGTACT
  ACATGAAGAATGGCGTGGACTTGGGGCCCATTTGTGGTCCCCCTAATGGTATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218109 representing NM_001159605
  Red=Cloning site Green=Tags(s)
  
  MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIG
  VAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNTSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGR
  AMGEGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSA
  NEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATV
  GLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLA
  AAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSATQQSAPQAQQGQQQVLRGGASQRPLTPN
  QNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLGAPVRLVAPAP
  VIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPSNNLASSSFYGNNSLSSNSQ
  SSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLAP
  IGHSFYSSLSYSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYR
  SASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFI
  QLKLERATAAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSHEQKLALAERIRGHVLSLALQMYGCR
  VIQKALEFIPSDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHP
  YGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQ
  HKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKI
  RPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159605
• ORF Size: 3276 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159605.1, NP_001153077.1
• RefSeq Size: 5079
• RefSeq ORF: 3279
• Locus ID: 80912
• MW: 116.6 kDa