Gja8 (NM_008123) Mouse Tagged ORF Clone

CAT#: MR218273

Gja8 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 8 (Gja8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gja8
• Synonyms: Aey5; Cnx50; Cx50; Lop10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218273 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGTTTCCTGGGAAACATCTTGGAAGAGGTGAATGAGCACTCCACTGTCATCGGCAGAG
  TCTGGCTCACAGTGCTCTTCATCTTCCGCATCCTCATCCTCGGGACAGCAGCGGAGTTTGTGTGGGGCGA
  TGAGCAATCTGATTTTGTATGCAACACCCAGCAGCCAGGCTGTGAGAATGTCTGCTACGATGAGGCCTTT
  CCCATCTCACACATCCGCCTCTGGGTGCTGCAGATCATCTTCGTCTCCACTCCATCGCTGATGTACGTGG
  GGCACGCGGTACACCACGTTCGCATGGAGGAGAAGCGAAAGGACCGTGAAGCTGAGGAGCTCTGTCAGCA
  GTCGCGCAGCAACGGGGGTGAGAGGGTACCAATCGCCCCAGACCAGGCCAGCATCCGGAAGAGCAGCAGC
  AGTAGCAAAGGCACCAAGAAGTTCCGGCTGGAGGGCACACTGCTAAGGACCTATGTCTGCCACATCATCT
  TCAAGACCCTCTTTGAGGTGGGCTTCATCGTGGGCCATTACTTCCTGTATGGTTTCCGCATCCTGCCCCT
  CTATCGCTGCAGCCGGTGGCCCTGCCCCAATGTGGTAGACTGCTTTGTATCCCGGCCTACTGAGAAGACC
  ATCTTCATCCTCTTCATGTTATCAGTCGCTTTTGTGTCACTCTTCCTCAACATCATGGAGATGAGCCACC
  TGGGCATGAAAGGAATCCGGTCTGCCTTCAAGAGGCCTGTAGAGCAACCACTGGGGGAGATTGCTGAGAA
  GTCCCTCCACTCCATTGCAGTTTCCTCCATCCAGAAAGCCAAGGGCTACCAGCTTCTAGAAGAAGAGAAG
  ATCGTATCACACTATTTCCCTTTGACAGAGGTTGGAATGGTGGAGACCAGCCCTCTTTCGGCCAAGCCTT
  TTAGTCAGTTTGAGGAGAAGATCGGCACAGGACCCCTGGCAGATATGTCACGGAGTTACCAAGAAACCCT
  GCCTTCTTATGCTCAGGTGGGGGTCCAGGAAGTGGAGCGGGAAGAGCCGCCTATAGAAGAGGCTGTGGAA
  CCGGAAGTGGGAGAGAAGAAGCAAGAAGCAGAGAAGGTGGCCCCAGAAGGGCAGGAGACAGTTGCAGTGC
  CAGACAGGGAGAGAGTAGAGACCCCTGGAGTGGGGAAGGAGGATGAGAAAGAAGAGCTGCAAGCTGAAAA
  GGTAACCAAGCAAGGGCTGTCTGCTGAGAAGGCACCCTCACTCTGTCCGGAGCTGACAACCGATGACAAT
  CGGCCCTTGAGCAGGCTGAGTAAAGCCAGCAGCAGGGCCAGGTCAGATGATCTCACCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218273 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGCENVCYDEAF
  PISHIRLWVLQIIFVSTPSLMYVGHAVHHVRMEEKRKDREAEELCQQSRSNGGERVPIAPDQASIRKSSS
  SSKGTKKFRLEGTLLRTYVCHIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKT
  IFILFMLSVAFVSLFLNIMEMSHLGMKGIRSAFKRPVEQPLGEIAEKSLHSIAVSSIQKAKGYQLLEEEK
  IVSHYFPLTEVGMVETSPLSAKPFSQFEEKIGTGPLADMSRSYQETLPSYAQVGVQEVEREEPPIEEAVE
  PEVGEKKQEAEKVAPEGQETVAVPDRERVETPGVGKEDEKEELQAEKVTKQGLSAEKAPSLCPELTTDDN
  RPLSRLSKASSRARSDDLTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008123
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008123.1, NM_008123.2, NM_008123.3, NP_032149.1
• RefSeq Size: 6989
• RefSeq ORF: 1323
• Locus ID: 14616
• MW: 49.6 kDa
• Gene Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProtKB/Swiss-Prot Function]