Pak6 (NM_001033254) Mouse Tagged ORF Clone

CAT#: MR218274

Pak6 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pak6
• Synonyms: 4732456M09
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218274 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCAAGAAAAAGAAGAAACGTCCTGAGATATCAGCCCCGCAGAACTTCCAGCATCGTGTCCACA
  CCTCCTTTGACCCCAAGGAAGGCAAGTTTGTGGGTCTGCCCCCACAATGGCAGAACATTCTGGATACTCT
  GAGGCGGCCCAAGCCTGTGGTGGATCCTTCTCGAATCACACGGGTGCAGTTGCAGCCCATGAAGACAGTC
  GTACGGGGCAGCTCAGTGCCCACGGAGGGCTACATCTCGGGGCTGCTCAACGACATTCAGAAGTTGTCAG
  TTATCAGCTCCAACACCCTGCGTGGACGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT
  GGGAGATGATCAGTGGGCTGCTGACCCCGATATGTACCTCCAAAGCCCCCAGTCGGAGCACACTGATCCC
  CATGGCCTCTACCTCAGCTGCAATGGGGGCACACCAGCAGGTCACAGGCAGGTGCCCTGGCCAGAACCAC
  AGAGCCCGCAGGCCCTGCCCAATGGGATGGCTGCCAAGGCGCAGTCACTGGGCCCTGCTGAGTTCCAGGG
  TGCCTCACAACGCTGCCTGCAGCAGCTGGGTGCCTGCCTGCAGAGTTCCCCACCCGGTACCTCACTCCCT
  ATGGCCACAGGGAGGCGTGGGGTGAAGGTTGCCAAGCATAGCTCCGAAGAGGCCCGGCCACAGTCCTGCC
  TGGTGGGCTCGGCCATTGGCAGGCCAGGTGGAGAAGGCAGCCCTAGCCCTAAGAACCAGGAAAGCAGCCT
  CAAGCACCGGCTCTTCCGAAGCATGTTCCTGTCCACTCCTGCCACAGGCGCGGCAAGCAGCAGCAAGCCG
  GTCCCTCTGCCGCAGAACAAGCCCAATTCTGCTTTCCGACCACCACAAAAAGACTCCTCTTCAAACCTGG
  TAGCCAAGGCCCAGTCCTTGCCCTCAGAGCAGCCCATGGGGACCTTCAGTCCTCTGACCACCTCAGATAC
  CAGCAGCCCCCAGAAGTCCCTCCGCACAGTTCCAGCCGCTGGTCCACTTCCAGGCCGGTCTTCTCCAGCA
  GGCTCCCCCCGTACCCGGCATGCTCAGATCAGCACCAGCAATCTCTACCTGCCCCAGGACCCCACAGTGG
  CCAAGGGGGCCCTGGGTGGCGAAGACACGGGCATTGTGACCCATGAACAGTTCAAGGCTGCGCTCAGGAT
  GGTGGTGGACCAGGGTGACCCTCGGCTGCTGCTGGACAGCTACGTGAAGATTGGGGAGGGCTCCACGGGC
  ATTGTGTGTCTGGCCCGGGAGAAGCACTCAGGTCGCCAGGTAGCAGTCAAGATGATGGACCTCAGGAAGC
  AGCAACGCAGGGAGCTGCTCTTTAATGAGGTGGTGATCATGCGTGACTACCAGCACCTCAACGTGGTGGA
  GATGTACAAGAGTTACCTGGTAGGAGAGGAGCTGTGGGTGTTGATGGAGTTCCTGCAGGGCGGGGCCCTC
  ACAGACATCATCTCCCAAGTCAGACTGAATGAGGAGCAGATTGCCACTGTGTGTGAAGCTGTGCTTCAGG
  CCTTGGCTTACCTGCACGCCCAGGGTGTCATCCACCGAGATATCAAGAGTGATTCCATCCTGCTGACCCT
  TGATGGCAGGGTGAAGCTCTCAGACTTTGGGTTCTGTGCTCAGATCAGCAAAGATGTTCCCAAGAGAAAG
  TCCCTGGTAGGAACTCCATACTGGATGGCTCCTGAAGTGATCTCCAGATCCCTGTACGCTACTGAGGTGG
  ATATCTGGTCTCTGGGCATCATGGTGATTGAGATGGTGGATGGAGAACCCCCCTACTTCAGTGACTCCCC
  AGTGCAAGCCATGAAGAGGCTGCGGGACAGCGCCCCACCCAAGCTAAAGAACTCTTACAAGGTCTCTCCA
  GTGCTGCGAGACTTCCTGGATCGGATGCTGGTACGGGAACCCCAAGAGCGAGCCACGGCCCAGGAGCTCC
  TGGATCACCCGTTTCTGCTGCAGACGGGGCTGCCTGAGTGTCTGGTGCCTCTGATACAGCTCTACCGCAA
  GCAGACCTCCACCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218274 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTV
  VRGSSVPTEGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDDQWAADPDMYLQSPQSEHTDP
  HGLYLSCNGGTPAGHRQVPWPEPQSPQALPNGMAAKAQSLGPAEFQGASQRCLQQLGACLQSSPPGTSLP
  MATGRRGVKVAKHSSEEARPQSCLVGSAIGRPGGEGSPSPKNQESSLKHRLFRSMFLSTPATGAASSSKP
  VPLPQNKPNSAFRPPQKDSSSNLVAKAQSLPSEQPMGTFSPLTTSDTSSPQKSLRTVPAAGPLPGRSSPA
  GSPRTRHAQISTSNLYLPQDPTVAKGALGGEDTGIVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTG
  IVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHLNVVEMYKSYLVGEELWVLMEFLQGGAL
  TDIISQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRK
  SLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSAPPKLKNSYKVSP
  VLRDFLDRMLVREPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033254
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033254.1, NM_001033254.2, NM_001033254.3, NP_001028426.2
• RefSeq Size: 4137 bp
• RefSeq ORF: 2049 bp
• Locus ID: 214230
• Cytogenetics: 2 E5
• MW: 74.9 kDa