Cerk (NM_145475) Mouse Tagged ORF Clone

CAT#: MR218633

Cerk (Myc-DDK-tagged) - Mouse ceramide kinase (Cerk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cerk
• Synonyms: AI848610; D330016D08Rik; mKIAA1646
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218633 representing NM_145475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCAATGGGGGCGGCGGAGCCGCTGCACTCCGTGCTGTGGGTGAAACGGCGACGCTGTGCCGTCA
  GCCTGGAGCCCGCGCGGGCTCTGCTACGCTGGTGGCGGAGCCCGGAGCCCGGGCCCTCCGCGCCGGGTGC
  CGATGCCCGCTCGGTACTGGTGTCGGAGATCATCGCTGTTGAGGAAAAAGACGACTGCGAGAAACACGCC
  TCCAGTGGCCGATGGCATAAAATGGAAAATCCGTTCGCATTCACAGTCCACCGTGTGAAGCGAGTACGAC
  ATCACCGCTGGAAGTGGGCACGGGTGACCTTCTGGAGCGCCGACGAGCAGCTGTGTCACCTGTGGCTGCA
  GACCCTCCGTGGGCTGCTGGAGAGCCTGACTTCAAGACCGAAGCACTTGCTGGTATTCATCAACCCTTTC
  GGAGGGAAAGGTCAGGGCAAGCGCATCTATGAAAAAACAGTGGCGCCTCTGTTTACCTTGGCTTCCATCA
  CTACGGAGATCATCATTACAGAGCATGCCAACCAAGCCAAGGAGACTTTATACGAGATCAACACAGACAG
  CTATGATGGCATCGTGTGCGTAGGTGGGGACGGCATGTTCAGCGAGGTGCTGCATGGGGTGATTGGGAGG
  ACGCAGCAGAGCGCTGGTATCGACCCCAATCACCCCCGAGCCGTGCTGGTGCCCAGTACCCTCAGGATCG
  GCATCATACCCGCAGGGTCCACAGATTGTGTGTGTTACTCAACAGTGGGCACAAACGACGCAGAGACATC
  GGCTTTGCACATCATTATTGGGGACTCACTGGCAATAGACGTGTCCTCTGTGCACTACCATAACACGCTG
  CTGCGGTACTCGGTTTCTCTGCTGGGCTACGGTTTCTACGGGGACTTAATCAAGGACAGTGAAAAGAAAC
  GGTGGATGGGCCTCGTCCGGTATGATTTCTCAGGGTTGAAGACCTTTCTCTCTCATCAGTACTATGAAGG
  GACACTGTCCTTCCTCCCAGCACAGCACACGGTGGGATCTCCACGGGACAATAAACCCTGCCGGGCTGGG
  TGCTTCGTGTGCAGGCAGAGCAAGCAACAGCTGGAAGAAGAAGAGAAGAAAGCCCTGTATGGCCTGGAGA
  ACGCCGAGGAAATGGAAGAGTGGCAAGTGACATGTGGGAAGTTCCTGGCCATCAATGCCACCAACATGTC
  CTGTGCTTGTCCTCGGAGCCCTGGGGGCCTGTCCCCATTTGCCCATCTGGGAGATGGGTCTTCTGACCTC
  ATCCTTATCCGGAAGTGCTCCAGGTTCAACTTCCTGAGATTCCTCATCCGGCACACGAACCAGGAGGACC
  AGTTCGACTTCACTTTCGTTGAAGTTTATCGAGTCAAGAAATTCCACTTCACGTCGAAGCACGTGGAAGA
  CGAGGACAATGACTCGAAGGAACAAGAGAAGCAGAAGTTTGGGAAGATCTGCAAGGACAGACCCTCTTGC
  ACTTGCTCAGCCTCCAGAAGCTCCTGGAACTGCGATGGCGAAGTCATGCACAGCCCGGCCATTGAGGTCA
  GGGTCCACTGCCAGCTGGTGCGCCTCTTTGCTCGGGGAATCGAGGAAGAGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218633 representing NM_145475
  Red=Cloning site Green=Tags(s)
  
  MGAMGAAEPLHSVLWVKRRRCAVSLEPARALLRWWRSPEPGPSAPGADARSVLVSEIIAVEEKDDCEKHA
  SSGRWHKMENPFAFTVHRVKRVRHHRWKWARVTFWSADEQLCHLWLQTLRGLLESLTSRPKHLLVFINPF
  GGKGQGKRIYEKTVAPLFTLASITTEIIITEHANQAKETLYEINTDSYDGIVCVGGDGMFSEVLHGVIGR
  TQQSAGIDPNHPRAVLVPSTLRIGIIPAGSTDCVCYSTVGTNDAETSALHIIIGDSLAIDVSSVHYHNTL
  LRYSVSLLGYGFYGDLIKDSEKKRWMGLVRYDFSGLKTFLSHQYYEGTLSFLPAQHTVGSPRDNKPCRAG
  CFVCRQSKQQLEEEEKKALYGLENAEEMEEWQVTCGKFLAINATNMSCACPRSPGGLSPFAHLGDGSSDL
  ILIRKCSRFNFLRFLIRHTNQEDQFDFTFVEVYRVKKFHFTSKHVEDEDNDSKEQEKQKFGKICKDRPSC
  TCSASRSSWNCDGEVMHSPAIEVRVHCQLVRLFARGIEEES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145475
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145475.1, NM_145475.2, NM_145475.3, NM_145475.4, NP_663450.3
• RefSeq Size: 4562
• RefSeq ORF: 1596
• Locus ID: 223753
• MW: 59.8 kDa