Brwd1 (NM_001103179) Mouse Tagged ORF Clone

CAT#: MR218689

Brwd1 (Myc-DDK-tagged) - Mouse bromodomain and WD repeat domain containing 1 (Brwd1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brwd1
• Synonyms: 5330419I02Rik; D530019K20Rik; G1-403-16; repro5; Wdr9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218689 representing NM_001103179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCTTCGCCGGCCCGACGTCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTTCTCATCGCCC
  GCTACCTCTCAGCTGGCCCGTGTCGCCGAGCGGCGCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGCT
  GTTGCCGAAGAGGTTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAATTGGTCTTGTCCAAT
  AAACATGTGGCTCCTGACCACCTACTACAGATATGCCAGCGCATCGGTCCGATGCTGGATAAGGAGGTCC
  CGCCCAGTATCTCAAGAGTCACTTCTTTGCTTGGTGCAGGAAGGCAGTCTTTGCTACGAACAGCAAAAGA
  CTGCAGGCACACAGTGTGGAAGGGCTCTGCCTTTGCTGCTCTTCACAGAGGAAGACCTCCTGAAATGCCC
  GTCAATTATGGCCCCCCACCGAGTCTTGTGGAGATACACCGAGGAAGGCAGCTCACAGGGTGTTCCACTT
  TTAGCACAGCGTTCCCAGGAACTATGTACCAGCATATAAAGATGCACAGGAGGATTCTTGGACACCTGTC
  TGCAGTGTACTGTGTAGCATTTGATAGGACAGGACATAGAATCTTTACAGGTTCAGATGATTGCTTGGTA
  AAGATTTGGTCAACACATAATGGCCGCTTGTTGTCTACGTTAAGAGGCCATTCTGCAGAGATTTCAGACA
  TGGCTGTGAACTACGAGAACACGCTGATTGCTGCAGGAAGCTGTGATAAGATCATTAGGGTGTGGTGCTT
  GAGGACCTGCGCCCCAGTTGCTGTGCTGCAAGGACACACGGGGTCAATCACATCTCTGCAGTTTAGCCCG
  ATGGCCAAAGGACCTCAGCGGTACATGGTTTCTACGGGCGCTGATGGCACTGTTTGCTTCTGGCAGTGGG
  ATTTAGAGTCCTTGAAGTTCAGTCCACGACCCCTGAAGTTCACGGAAAAGCCAAGGCCTGGTGTTCAGAT
  GCTGTGTTCTTCATTTAGCGTTGGTGGTATGTTTTTAGCTACAGGTAGTACTGATCATGTAATCAGAATG
  TATTTTTTGGGATTCGACGCACCTGAAAAAATCGCCGAGCTTGAAAGCCACACTGACAAAGTTGATAGTA
  TCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTT
  TGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAA
  GAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAG
  CCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACA
  TGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGAT
  GGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGAC
  AAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCA
  CGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTT
  CATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCAC
  CTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATT
  AGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGAT
  GGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCG
  TCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGAC
  TGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATT
  CAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACG
  CTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGC
  ACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTAT
  GTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGT
  CTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATC
  TTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCT
  TCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCAT
  CTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCG
  TCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGA
  AGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTG
  CAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCC
  CTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTC
  AGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAG
  AACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATT
  TATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTT
  ATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGAT
  TTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTA
  TCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGG
  GACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAG
  ATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTG
  TGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTG
  GATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGA
  GGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGC
  AAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAG
  TTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACA
  GTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAA
  AAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTC
  ATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGATTACCGAG
  ATATTATAGATACGCCAATGGATTTTGGAACAGTAAGGGAAACTCTAGAAGCTGGGAATTACGATAGCCC
  TGTGGAGTTTTGCAAAGACATTCGATTGATATTTAGCAATGCAAAGGCATATACACCGAACAAAAGATCA
  AAGATTTATAGTATGACCTTGAGATTGTCTGCTTTGTTTGAAGAAAAAATGAAGAAAATCTCCTTTGATT
  TTAAAATTGGTCAAAAATTCAAGGAGAAACTTCGTAGAAGCCAAAGATTCAAGCAACGACAAAACTGTAA
  CGGTGCTGTCCCTGGTGACAGGCGCAGGAGAAATGTCAAGCAGAAGCAGTTCAAATCTCAGACAAAAGTA
  ATTCCACAGTTGATGTGTCCTCCTTCTCAGTCTACCTCAAGTAAGGTACCTTTGTCTGCAACCCGCAAGA
  CAAGTGCTGGTGTCTCTTCAGGCTTTACTTCTGGGGACTCTTCAGACTCTGCAGGATCTTTAGAAAGAGT
  CAGAAGACAGAGGCCTGAAGTACTCAGGAGTGGTTCTGTGCTGTTTGGAAGTGAAATGGAAGATTTCTTA
  GCTACCTCTTCATCTTCTTCAGCTTCAAACAGTTCAGAGGAAAGCAAAGCGAGTCCTGGAGCTCGAGAAT
  CTTCCTTGCGCAGTGGTGTGTTGAGAGGCAGCAACCTCGGGGTGACCAGAACAAGAGCTGCTCGGAGGAA
  AGCTGGTTCTGTTTCTTTAGAAAATGGGTGTGGTAGAAAAGCCACTCGGAAGAGAGTCTATTTAAGTGAT
  TCTGATAACAATTCAGTGGAAACAGATGAAAATCTGAAAAATAGAAAATGTGGAAGTAGCCGGAAAGTTC
  TGCGGAAGTGTGCTGCTGTTGCTGCCAGCAAGATAAAGCTGATGAGTGATGCAGAGGATTCGAGCTCAGA
  GAGCCCCTGCTCTGGGCGGAAGCTGCCACATCGAAACGCTTCAGCTGTGGCCAGGAAGAAGTTGCTACAC
  AACTCTGATGATCAGAGCCTTAAGTCAGAGACAGAAGAACTGAAAGACCAGAACCAATCACTGTTGATAT
  CCGGTCCCCATTCTGTCCACAACAGCATCAGTGACTCAGAGTCAGACAGTGACTTGCGAGCAACCCGGAA
  GACCTGGAATGCTAACGGCTGCACATCCCACACTGCAGCAACATGCAAAACCAAGTCACGTCCAATAGAG
  TCTTCTGAGGAAGATTCCAGATGTCATGGATCTGATCATGGGCCCAGCAGCACTGGGGACCCTTCAACAT
  CTGGGCAGAAACTCAGGGCAGATAGCATCTCTGAGGAAGCTGACTCTGAACCAGAAAGCAGTGTACTATG
  CAAGAATACACATCTGTGTAAGAAAGCCAAGATCTTGAGTGACTCAGAAGACTGTGAGGAAAAGTGTGGA
  GAAAGGAGAGGTCCTGAAGTGGAAGGAAGCCCAGTTTCTGAAGCCTTGAGGGAAGCCATTCTTGCCCCCC
  AGTGTCTCTCACATCGTGGATCTGAGACTGATGTGGATTCTGACGGCGGTGCAGTGAGAGAGAAATCATA
  CAGCAATGAAAACGGATCTGTATCACTAGAGAATGGACAAAGAAGAAAAGTTTCCCGAAAAAGTTCCAGT
  GACAAGGAAAGTAATCTACAGGTAACCCAGAAGTCACCCAAAGACAGAAGCAGCCCCTCGAGGATTACTC
  AGCGTGCTAGTGTGGCTACTGATAAAATGAAGCTCACAAGTGATGCAGAAGACCTCAGCCTAGAGAGCGT
  GTGTACTCGAAGCAAAAGGAGAAGGAAAAAGCCTGCTCGATTTGCTTGTACCCCAGCAAAGACTGCATTG
  AGTAGTGAGGAGAAACACGCACATTGTGAAGTGCCTGAGGCACAGCCTGCCTGCAGGAACAAGCTCCCTG
  AGCCTGAACACCAAGATAGTGCAGAGAATCCCAGTCAGGCTGCAAGTGCAGACTTGAATTCTGGAGGGGG
  TTCCAGTTTTGAACAAAGAAAAAGCATACAGAGCAGGCAAATGGGCGCTGTCTGCGTAAGGCCACCTCCT
  AAAACCCAAAGCTCCTCTGCTGGGCTTTCTCAGGAAAACGCCAGAAGCCAAACCCTAGATAGTGAGACTA
  GCTTGCCTTCTGAGTCGGTTTTGACCCAGAAAGCTACTGTAGAGAGTAACTTTGAAGAAGAACTGAACTA
  TGGATTAAGAAGGTGGAATGGCAGAAGGCTCAGGACATACGGGAAGGCCCCACTCAGTAGGACAGCACAA
  GTTACTCCCAGTTTACAGGCCTCAGCAGAGGTCGGGGTGAAGAGGAGGAGAATGCATCCTGAGGTAGATG
  GTGAGGACGTGCCCGGACAAATGGGGAGTTCAGGATGCGGACCTGACACCAGTCCCAAGGCATCAGACTT
  GGGGTCAGTAACTGACTCTGATGTTGACTGTACCGATAACACACAAACCCAAAGGAAGAAGAAAAGGAAA
  GGGAAAGCAAGAGTGCTTAGCAAAGGTAAACCCTCCTCATCCAACTCACCTAAAACTGCAAGACATCCAA
  GGCAGAGTAAAGGTCCTAGGTTAAATGTGGATGACAATGACTGGGAGGATTTGGACTATGCAAAAGCTAA
  AGGAGTTGTCCGGCTCTCAAAAATCCAAACGAGAAATCAGGGTAGGAGAACGGTGAGATACCACGATGGG
  GAAGATGATAGAAGCATAGAGAGCGCACTAGAGCTCACTGACCGCACCTTACGCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218689 representing NM_001103179
  Red=Cloning site Green=Tags(s)
  
  MAEPSPARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSN
  KHVAPDHLLQICQRIGPMLDKEVPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMP
  VNYGPPPSLVEIHRGRQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLV
  KIWSTHNGRLLSTLRGHSAEISDMAVNYENTLIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSP
  MAKGPQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRM
  YFLGFDAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMSARISGDTSSE
  EERFMKPKVTMIAWNQDDSTVVTAVNDHVLKVWSSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHD
  GSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFF
  HTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSD
  GEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDI
  QSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLY
  VIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRHETSYDQSEGSCS
  SEDDEWRNDRRSGSDSDSSSDSSSQYSDWTADTGINLQPPLRMSCRRRVTRFCSTSEDEMSMENISPPKR
  RRKRRKESKPKRESLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVMYFRQGHEAYIEAV
  RRNNIYELNPHKEPWRKMDLRDQELVKIVGLRYEVGPPTLCCLKLAFIDPATGRLTDKSFSIRYHDMPDV
  IDFLVLRQFYDEARQRNWQPCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTETEKLSPW
  DMEPIPDNVDPPEELGASISVTSDELEKLLYKPQEGEWGQRSRDEECDRIISGIDQLLNLDIAAAFAGPV
  DLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALIWEVRYIEHNARTFNEPESVIARSAKKITDQLLK
  FIKNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRKVHNWKRRSRASGCIESDWRRQCKALLIL
  IFQCEDSEPFRQPVDLDEYPDYRDIIDTPMDFGTVRETLEAGNYDSPVEFCKDIRLIFSNAKAYTPNKRS
  KIYSMTLRLSALFEEKMKKISFDFKIGQKFKEKLRRSQRFKQRQNCNGAVPGDRRRRNVKQKQFKSQTKV
  IPQLMCPPSQSTSSKVPLSATRKTSAGVSSGFTSGDSSDSAGSLERVRRQRPEVLRSGSVLFGSEMEDFL
  ATSSSSSASNSSEESKASPGARESSLRSGVLRGSNLGVTRTRAARRKAGSVSLENGCGRKATRKRVYLSD
  SDNNSVETDENLKNRKCGSSRKVLRKCAAVAASKIKLMSDAEDSSSESPCSGRKLPHRNASAVARKKLLH
  NSDDQSLKSETEELKDQNQSLLISGPHSVHNSISDSESDSDLRATRKTWNANGCTSHTAATCKTKSRPIE
  SSEEDSRCHGSDHGPSSTGDPSTSGQKLRADSISEEADSEPESSVLCKNTHLCKKAKILSDSEDCEEKCG
  ERRGPEVEGSPVSEALREAILAPQCLSHRGSETDVDSDGGAVREKSYSNENGSVSLENGQRRKVSRKSSS
  DKESNLQVTQKSPKDRSSPSRITQRASVATDKMKLTSDAEDLSLESVCTRSKRRRKKPARFACTPAKTAL
  SSEEKHAHCEVPEAQPACRNKLPEPEHQDSAENPSQAASADLNSGGGSSFEQRKSIQSRQMGAVCVRPPP
  KTQSSSAGLSQENARSQTLDSETSLPSESVLTQKATVESNFEEELNYGLRRWNGRRLRTYGKAPLSRTAQ
  VTPSLQASAEVGVKRRRMHPEVDGEDVPGQMGSSGCGPDTSPKASDLGSVTDSDVDCTDNTQTQRKKKRK
  GKARVLSKGKPSSSNSPKTARHPRQSKGPRLNVDDNDWEDLDYAKAKGVVRLSKIQTRNQGRRTVRYHDG
  EDDRSIESALELTDRTLRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001103179
• ORF Size: 6777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001103179.1, NP_001096649.1
• RefSeq Size: 7547
• RefSeq ORF: 6780
• Locus ID: 93871
• MW: 254 kDa