Gsdme (NM_018769) Mouse Tagged ORF Clone

CAT#: MR218826

Dfna5 (Myc-DDK-tagged) - Mouse deafness, autosomal dominant 5 (human) (Dfna5)

Product Images

Coomassie blue staining of purified Gsdme protein (Cat# TP518826). The protein was produced from HEK293T cells transfected with Gsdme cDNA clone (Cat# MR218826) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gsdme
• Synonyms: 2310037D07Rik; 4932441K13Rik; Dfna5; Dfna5h; EG14210; Fin15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218826 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCCAAAGCAACTCGAAATTTTCTTAAAGAAGTTGATGCTGGAGGAGACCTGATTTCAGTCTCAC
  ACTTGAACGACTCTGACAAGCTGCAACTTCTAAGTCTGGTGACCAAAAAGAAGAGATACTGGTGCTGGCA
  GAGACCCAAGTACCAGATTTTATCTGCCACCCTGGAAGATGTACTCACAGAAGGGCACTGTCTCAGTCCA
  GTGGTTGTGGAGTCAGACTTCGTGAAATACGAGAGCAAGTGTGAGAACCATAAGAGCGGGGCTATTGGGA
  CAGTCGTGGGGAAGGTCAAGCTGAACGTTGGTGGCAAAGGCGTGGTGGAGAGTCACTCTTCGTTTGGAAC
  CCTGAGGAAGCAGGAGGTGGACGTGCAGCAGCTCATCCAGGATGCCGTCAAGAGAACAGTTAATATGGAC
  AACCTGGTACTTCAGCAGGTGCTAGAGAGCAGGAACGAGGTCCTGTGTGTGCTGACGCAGAAGATCATGA
  CCACGCAGAAGTGCGTGATTTCTGAGCATGTGCAGTCGGAGGAGACGTGTGGAGGCATGGTGGGGATCCA
  GACCAAGACTATACAGGTGTCAGCAACGGAGGATGGGACTGTCACCACAGACACCAATGTAGTGCTGGAG
  ATCCCTGCTGCCACCACCATTGCCTATGGCATCATGGAGCTGTTTGTGAAACAAGATGGCCAGTTTGAAT
  TCTGCCTCCTCCAAGGGAAACATGGTGGCTTCGAGCATGAGAGGAAACTAGACTCTGTCTACTTGGACCC
  CCTGGCCTACAGAGAGTTCGCCTTTCTGGACATGCCGGATGGGGGTCAAGGGATCTCTTCGCAGGACGGG
  CCCCTACGAGTTGTAAAACAAGCAACCCTGCACCTAGAGAGGAGTTTCCACCCCTTTGCGGTGTTACCTG
  CCCAGCAGCAGAGGGCGCTGTTCTGTGTCCTGCAGAAAATCCTGTTTGATGAAGAACTCCTTCGGGCCCT
  GGAGCAAGTGTGTGATGATGTGGCTGGTGGCCTCTGGTCCTCACAGGCTGTATTGGCAATGGAGGAGCTG
  ACCGACAGTCAGCAGCAGGACCTCACAGCCTTCTTGCAGCTGGTGGGATACAGGATACAAGGAGAGCATC
  CTGGCCCACAGGATGAGGTCAGCAACCAGAAGCTCTTTGCAACAGCCTACTTCCTGGTCAGCGCACTAGC
  AGAAATGCCTGATAATGCCACAGTTTTCCTGGGGACTTGCTGCAAACTCCATGTTATTTCTTCGCTGTGC
  TGCTTGCTCCATGCTCTGTCTGATGACAGCGTGTGTGATTTTCACAACCCCACCTTGGCTCCTCTGAGAG
  ACACAGAGAGGTTTGGCATTGTGCAGCGATTGTTTGCCTCTGCTGACATTGCCCTGGAGAGGATGCAGTT
  TTCTGCGAAAGCCACCATCCTGAAGGACTCTTGCATCTTCCCGCTAATTCTTCACATCACTCTAAGTGGG
  CTCAGCACTCTAAGCAAAGAGCATGAGGAAGAGCTTTGTCAGTCAGGACATGCTACAGGTCAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218826 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAKATRNFLKEVDAGGDLISVSHLNDSDKLQLLSLVTKKKRYWCWQRPKYQILSATLEDVLTEGHCLSP
  VVVESDFVKYESKCENHKSGAIGTVVGKVKLNVGGKGVVESHSSFGTLRKQEVDVQQLIQDAVKRTVNMD
  NLVLQQVLESRNEVLCVLTQKIMTTQKCVISEHVQSEETCGGMVGIQTKTIQVSATEDGTVTTDTNVVLE
  IPAATTIAYGIMELFVKQDGQFEFCLLQGKHGGFEHERKLDSVYLDPLAYREFAFLDMPDGGQGISSQDG
  PLRVVKQATLHLERSFHPFAVLPAQQQRALFCVLQKILFDEELLRALEQVCDDVAGGLWSSQAVLAMEEL
  TDSQQQDLTAFLQLVGYRIQGEHPGPQDEVSNQKLFATAYFLVSALAEMPDNATVFLGTCCKLHVISSLC
  CLLHALSDDSVCDFHNPTLAPLRDTERFGIVQRLFASADIALERMQFSAKATILKDSCIFPLILHITLSG
  LSTLSKEHEEELCQSGHATGQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018769
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018769.1, NM_018769.2, NM_018769.3, NP_061239.1
• RefSeq Size: 2133
• RefSeq ORF: 1539
• Locus ID: 54722
• MW: 56.6 kDa