Arhgap25 (NM_175476) Mouse Tagged ORF Clone

CAT#: MR218966

Arhgap25 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 25 (Arhgap25), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap25
• Synonyms: A130039I20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218966 representing NM_175476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGGGAGCAGATGGCGGCCTTTCACCCACCCACCACTCCCAACCCCCTGGAAAGGCCAATTAAGG
  TGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAAAACTGGCAGCAGCGGTACTTTGTGTTGAGAGCTCA
  GCAACTCTACTACTACAAGGACGAAGAGGACTCAAAGCCACAGGGCTGCATGTATCTGCCAGGCAGTACA
  GTCAAAGAAATTGCCACAAACCCCGAAGAAGCTGGGAAATTTGTCTTTGAAGTTATTCCAGCCTCAAGTG
  ACCAGAACCGCATTGGACAAGACTCCTACGTCCTCATGGCCAGCTCCCAGGTAGAGATGGAGGAGTGGGT
  TAAGTTCCTCAGGAGAGTTGCTGGCACGCCCTCTGGAGCGGTGTTTGGCCAGCGTCTGGATGAGACTGTG
  GCCTATGAGCAGAAGTTTGGCCCTCACCTGGTGCCCATCTTGGTGGAGAAGTGCGCTGAGTTCATCCTGG
  AACATGGTGTGAGTGAAGAGGGCATCTTCCGCCTGCCCGGGCAGGACAACCTTGTGAAGCAGCTGAGAGA
  TGCTTTCGATGCGGGGGAGCGGCCCTCCTTTGACAGGGACACAGATGTGCACACGGTGGCATCTCTACTA
  AAGCTCTACCTCCGAGACCTGCCAGAGCCTGTGGTTCCTTGGAGTCAGTATGAAGGGTTCCTGCTCTGTG
  GGCAGCTCATGAACGCAGATGAGGCAAAGGCTCAGCAGGAGTTGGTGAAGCAGCTTTCTACCCTTCCCCG
  AGACAACTACAACCTCCTGAGCTACATCTGCAGATTTCTGCATGAAATCCAGCTGAACTGTGCCGTCAAC
  AAGATGAGCGTGGACAACCTGGCCACTGTGATCGGGGTGAACCTCATCAGGTCGAAGGTTGAAGACCCAG
  CTGTGATTATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACCATGATGATCAGAGACCACGAAGTCCT
  CTTCCCCAAGTCTAAGGATGCACCGATCTCACCCCCTGCCCAGAAAAACGATGCCAAGAAGGCTCCAGTG
  CCGCGAAGCTCTGTGGGGTGGGATGCCACGGAAGACCCACCCCTTTCTAGGACAGACAGCTTCAGTAACA
  CAGCAAGTAGTCCTGATGCCACCAGTCCCACTGGACCACTGCCCAGTGACCAGCATCAGGAAGACAGCGG
  AAAAGCCCCCAGGGAAAACCCAGGAGACTGGAAGATGCAATCCCGTAAAAGGACTCAAACGCTTCCCAAC
  CGGAAGTGTTTCCTGACGTCCGCATTCCAAGGCACCACCAGCAGTAAACTGGAAATCTTTAAAAATGAGT
  TCTGGTCTCCATCTTCAGAGGCTAAGGCAGGAGAAGGGCACAGGCGAACTATGTCCCAAGACTTGCGCCA
  CCTTTCCAATGACCAGCGGACTTCTACCTACGATAATGTCCCCACCTCACCACAGTCCCAAGGGAACCCA
  GCAGGTGCACTCTCTCCCCCTGCCAGTGACTCCAAGAGAGATGCTCTTGTTAGCACAGACTCTGAAATGG
  AGGCTGGAAGCAAGAACTCTGGCGAGGATGACCTTGATTCTCTGCAGAGGACAGTCCAGAGCCTACAGAA
  GGAAATAGAAACCCAGAAGCAGGTCTATGAGGAACAGATTAAAAACCTGGAGAAGGAAAATTACGATGTC
  TGGGCTAAGGTGGTGAGGCTCAATGAAGAACTCGAGAGGGAGAGGAAGAAATTCGCGGCCCTGGAAATCA
  GCCTTCGAAATGTGGAGCGCTCCCGGGAGGACGTTGAGAAGAGGAACAGAGTCTTGGAAGAAGAAGTCAA
  GGAGTTTGTGAAGTCGATGGAGAAGCCCAAGACAAAGACGGATCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218966 representing NM_175476
  Red=Cloning site Green=Tags(s)
  
  MTGEQMAAFHPPTTPNPLERPIKVGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDSKPQGCMYLPGST
  VKEIATNPEEAGKFVFEVIPASSDQNRIGQDSYVLMASSQVEMEEWVKFLRRVAGTPSGAVFGQRLDETV
  AYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLL
  KLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVN
  KMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPV
  PRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPN
  RKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNP
  AGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDV
  WAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175476
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175476.3, NM_175476.4, NP_780685.2
• RefSeq Size: 2499
• RefSeq ORF: 1869
• Locus ID: 232201
• MW: 70.8 kDa