Acsl6 (NM_001033597) Mouse Tagged ORF Clone

CAT#: MR219239

Acsl6 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase long-chain family member 6 (Acsl6), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acsl6
• Synonyms: A330035H04Rik; AW050338; Facl6; LACS; Lacsl; mKIAA0837
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219239 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCTTCTTCTTGGTGTCGGGGGGTTCTCTCTGGCTGTTTGCCGAGATTGCTCTCTCACTTCTGG
  AGAAGATGCAGACCCAGGAGATCCTGAGGATCCTGCGGCTGCCCGAGCTATCGGACTTGGGCCAGTTTTT
  CCGCAGCCTCTCAGCTACCACCCTCGTGAGTGTGGGTGCACTGGCTGCCGTCCTTGCCTACTGGCTCACT
  CACCGGCCAAAGGCCTTGCAACCACCATGCAACCTCCTGAAGCAGTCGGAAGAAGTGGAGGACGGTGGTG
  GAGCCCGGCGATCTGTGATTGGGGGTTGCACTCAATTGCTTACCCATTACTATGACGATGCCCGGACCAT
  GTACCAGGTGTTCCGCCGTGGGCTTAGCATCTCAGGGAATGGACCCTGTCTTGGCTTCCGGAAGCCAGAG
  CAACCTTACCAGTGGCTGTCCTACCAAGAGGTGGCCAAAAGGGCTGAATTTCTGGGGTCGGGGCTTCTCC
  AGCACGACTGTAAAGTGGGCACAGAGCAGTTCGTTGGTGTTTTTGCACAAAATCGGCCGGAGTGGATCAT
  TGCCGAGCTGGCCTGCTACACGTATTCCATGGTGGTGGTACCGCTCTACGACACCCTGGGCCCAGGGTCT
  ATCAGCTACATCATCAATACTGCGGACATTTGCACGGTAATCGTCGATAAACCCCACAAGGCAACACTTC
  TGCTGGAACACGTGGAGAGGAAGGAGACTCCGGGCCTCAAGCTGGTCATCCTCATGGAGCCGTTTGAGGA
  CGCCCTGAGAGAGAGAGGAAAGAAGTGCGGGGTGGACATCAAGTCCATGCAGGCTATAGAGGACTGTGGC
  CGAGAGAATCATCATGCCCCCGTGCCCCCACGGCCTGATGACCTCTCCATCGTGTGTTTCACAAGTGGTA
  CAACAGGGAACCCCAAAGGTGCAATGCTCACCCACGGGAACGTGGTGGCCGATTTCTCGGGCTTTCTGAA
  AGTGACAGAGAGTCAGTGGGCCCCGACCTGTGCGGACGTCCACTTTTCCTACTTGCCTTTAGCACACATG
  TTTGAGCGCATGGTGCAGTCTGTTGTCTACTGCCACGGAGGGCGCGTGGGCTTCTTCCAGGGAGACATCC
  GCCTCCTCTCAGATGACATGAAGGCTCTCCGCCCTACCATCTTCCCTGTGGTCCCACGGCTGCTGAATCG
  GATGTATGACAAGATCTTCCACCAGGCAGACACCTCACTAAAGCGCTGGCTCCTGGAGTTTGCAGCAAAG
  CGCAAGCAGGCAGAGGTCCGGAGCGGAATCATCAGAAACAATAGTATCTGGGATGAACTCTTCTTTAATA
  AGATTCAGGCCAGTCTTGGTGGGCATGTGAGGATGATTGTCACTGGAGCAGCCCCCGCGTCACCAACGGT
  TCTGGGATTCCTACGAGCAGCTCTGGGGTGCCAGGTCTATGAAGGTTATGGGCAAACTGAATGCACAGCT
  GGGTGTACCTTCACAACGCCAGGGGACTGGACATCAGGGCATGTAGGGGCACCTCTGCCCTGCAACCACA
  TCAAGCTGGTCGATGCAGAGGAACTCAACTACTGGACCTGCAAAGGAGAAGGAGAGATATGTGTGAAAGG
  ACCAAATGTGTTCAAAGGCTACTTAAAAGACGAGGACAGGACAAAGGAGGCCCTGGACAGCGACGGCTGG
  CTTCACACTGGAGACATTGGGAAATGGCTGCCGGAGGGAACACTCAAAATCATTGATCGGAAAAAGCACA
  TATTTAAACTTGCTCAGGGGGAATATGTTGCGCCAGAGAAGATCGAGAACATCTACATCCGGAGTGAGCC
  TGTGGCACAAATCTACGTCCACGGGGACAGCTTAAAGGCCTTTTTGGTTGGCATTGTCGTGCCTGACCCT
  GAAGTCATGCCTTCCTGGGCTCAGAAGAAAGGAATCGAAGGGACCTATCAGGAACTCTGCATGAAAAAGG
  AATTGAAGAAAGCCATTCTGGATGACATGGTGATGCTGGGGAAAGAAAGCGGACTGCATTCTTTTGAACA
  GGTTAAAGCCATTTACATCCATTGTGACATGTTCTCTGTTCAAAATGGTCTGCTGACACCAACACTAAAG
  GCTAAGAGACCGGAGCTGAGAGAGTACTTCAAAAAGCAAATAGAAGAGCTTTACTTAGTCTCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219239 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLTFFLVSGGSLWLFAEIALSLLEKMQTQEILRILRLPELSDLGQFFRSLSATTLVSVGALAAVLAYWLT
  HRPKALQPPCNLLKQSEEVEDGGGARRSVIGGCTQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPE
  QPYQWLSYQEVAKRAEFLGSGLLQHDCKVGTEQFVGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGS
  ISYIINTADICTVIVDKPHKATLLLEHVERKETPGLKLVILMEPFEDALRERGKKCGVDIKSMQAIEDCG
  RENHHAPVPPRPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTESQWAPTCADVHFSYLPLAHM
  FERMVQSVVYCHGGRVGFFQGDIRLLSDDMKALRPTIFPVVPRLLNRMYDKIFHQADTSLKRWLLEFAAK
  RKQAEVRSGIIRNNSIWDELFFNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTA
  GCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTCKGEGEICVKGPNVFKGYLKDEDRTKEALDSDGW
  LHTGDIGKWLPEGTLKIIDRKKHIFKLAQGEYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDP
  EVMPSWAQKKGIEGTYQELCMKKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLK
  AKRPELREYFKKQIEELYLVSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033597
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033597.1, NP_001028769.1
• RefSeq Size: 2592 bp
• RefSeq ORF: 2169 bp
• Locus ID: 216739
• Cytogenetics: 11 32.13 cM
• MW: 80.7 kDa