Uba1 (NM_009457) Mouse Tagged ORF Clone

CAT#: MR219278L4

Lenti ORF clone of Uba1 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: mGFP
• Symbol: Uba1
• Synonyms: A1S9; Sbx; Ube-1; Ube1x
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR219278).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_009457
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009457.3, NP_033483.1
• RefSeq Size: 4068
• RefSeq ORF: 3357
• Locus ID: 22201
• Gene Summary: This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]