Uba1 (NM_009457) Mouse Untagged Clone

CAT#: MC223393

Uba1 (untagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba1
• Synonyms: A1S9; Sbx; Ube-1; Ube1x
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223393 representing NM_009457
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCTCTATTCAGGCGTCTATGGTATACCCCAGACCCGGCCCTCTAAGGCCCCCGCGCTTCCGAGCT
  CCGCGCAAACTCTGGTTCTTTTTGCACGATCGAGGTGGTTTGCTCTTCCGTTGCCCTGTGGCTTCGGCTT
  GTCTCCAGAAGGAAGGCGAGGCTTCCGCCCAGCACTGGGGATGTCCAGCTCGCCGCTGTCCAAGAAACGT
  CGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCACAGTCTGCGCTGTCCGAAGTGT
  CCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGAAGCAGACATAGACGAGAGCCTTTACTCCCG
  GCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTCCAGACATCCAGCGTCCTTGTCTCAGGCTTG
  CGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTCACCCTACATGACC
  AAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTACCTTCGGGAGGAGGACATTGGTAAAAATCG
  AGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGCTATGTACCTGTCACTGCCTACACTGGGCCT
  CTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCCTCACCAACAGCCCCCTGGAAGCCCAGCTGC
  GAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGTGGTGGCAGATACAAGAGGCCTGTTTGGGCA
  ACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATG
  GTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCTGCCTTGATGAGGCCCGACATGGCTTTGAGA
  CTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGATCCAACTCAATGGATGTCAGCCCATGGAGAT
  CAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACTTCCAACTTCTCTGACTACATCCGTGGAGGC
  ATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTAAATCCTTGCCAGCATCACTGGTAGAGCCTG
  ACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGCCCAACTGCACATTGGCTTCCAAGCTCTGCA
  CCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGAAATGAGGAAGATGCAACAGAGCTGGTGGGC
  CTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAAAACAGAACAGCTTGGATGAAGACCTTATTC
  GGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCATAAATGCTTTCATTGGGGGCCTTGCTGCCCA
  GGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATCATGCAGTGGTTGTACTTTGATGCTCTTGAA
  TGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGTGCCTCCCACGTCAGAACCGTTACGATGGGC
  AGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAGCAAGCAAAAGTACTTCCTGGTGGGTGCAGG
  GGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATTGGGCTGGGTTGTGGAGAGGGTGGAGAGGTC
  GTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGAACCGACAGTTTCTCTTCCGGCCCTGGGATG
  TCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCGCCAGATGAATCCTTACATCCAGGTGACAAG
  CCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTATGATGATGATTTCTTCCAAAATTTGGATGGT
  GTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACATGGATCGCCGATGTGTGTACTATCGGAAGC
  CACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGTGCAGGTGGTAATCCCCTTCCTGACAGAATC
  CTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCCATTTGTACCCTGAAAAACTTTCCCAATGCC
  ATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAGGCCTTTTCAAGCAGCCAGCAGAAAATGTTA
  ATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATTGCGGCTGGCTGGTACCCAGCCATTGGAGGT
  GCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCACAGACTTGGGGAGACTGTGTGACCTGGGCC
  TGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGCAACTGCTGCACAACTTTCCTCCTGACCAGC
  TCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACGCTGTCCACACCCACTTACTTTTGATGTTAA
  CAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAACCTTTTTGCCCAGACCTATGGGTTGACTGGA
  TCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAGTACAAGTCCCAGAGTTCACCCCCAAGTCTG
  GTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGCCAATGCCTCTGTTGATGACAGCCGTCTTGA
  GGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCCGGATTTAAGATGTACCCCATTGATTTTGAG
  AAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGGCTGCATCCAATCTTCGGGCCGAAAACTATG
  ATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACAAC
  CACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTACAAGGTAGTTCAAGGGCACCAACAGCTCGAT
  TCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCTTTGGGTTTTCTGAACCTCTTGCTGCACCTC
  GTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCGCTTTGAAGTACAAGGGCTGCAGCCTAATGG
  TGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAGACAGAGCACAAATTGGAGATCACCATGCTG
  TCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAGCTGCTAAGCTCAAGGAACGATTGGATCAGC
  CGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCTGGGCCGCCATGTGCGGGCACTGGTGCTTGA
  GCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTCCCTTATGTCCGATATACCATTCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009457
• ORF Size: 3357 bp
• Insert Size: 3357
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_009457.4, NP_033483.2
• RefSeq Size: 4068
• RefSeq ORF: 3357
• Locus ID: 22201
• Gene Summary: This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) uses an alternate 5' exon structure and an upstream start codon, compared to variant 3. The encoded isoform (1) has a longer N-terminus, compared to isoform 2.