Uba1 (NM_009457) Mouse Untagged Clone

CAT#: MC223393

Uba1 (untagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, (10ug)


  "NM_009457" in other vectors (5)

Reconstitution Protocol

USD 1,430.00

6 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Uba1
Synonyms A1S9; Sbx; Ube-1; Ube1x
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223393 representing NM_009457
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCTCTATTCAGGCGTCTATGGTATACCCCAGACCCGGCCCTCTAAGGCCCCCGCGCTTCCGAGCT
CCGCGCAAACTCTGGTTCTTTTTGCACGATCGAGGTGGTTTGCTCTTCCGTTGCCCTGTGGCTTCGGCTT
GTCTCCAGAAGGAAGGCGAGGCTTCCGCCCAGCACTGGGGATGTCCAGCTCGCCGCTGTCCAAGAAACGT
CGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCACAGTCTGCGCTGTCCGAAGTGT
CCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGAAGCAGACATAGACGAGAGCCTTTACTCCCG
GCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTCCAGACATCCAGCGTCCTTGTCTCAGGCTTG
CGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTCACCCTACATGACC
AAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTACCTTCGGGAGGAGGACATTGGTAAAAATCG
AGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGCTATGTACCTGTCACTGCCTACACTGGGCCT
CTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCCTCACCAACAGCCCCCTGGAAGCCCAGCTGC
GAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGTGGTGGCAGATACAAGAGGCCTGTTTGGGCA
ACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATG
GTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCTGCCTTGATGAGGCCCGACATGGCTTTGAGA
CTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGATCCAACTCAATGGATGTCAGCCCATGGAGAT
CAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACTTCCAACTTCTCTGACTACATCCGTGGAGGC
ATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTAAATCCTTGCCAGCATCACTGGTAGAGCCTG
ACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGCCCAACTGCACATTGGCTTCCAAGCTCTGCA
CCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGAAATGAGGAAGATGCAACAGAGCTGGTGGGC
CTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAAAACAGAACAGCTTGGATGAAGACCTTATTC
GGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCATAAATGCTTTCATTGGGGGCCTTGCTGCCCA
GGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATCATGCAGTGGTTGTACTTTGATGCTCTTGAA
TGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGTGCCTCCCACGTCAGAACCGTTACGATGGGC
AGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAGCAAGCAAAAGTACTTCCTGGTGGGTGCAGG
GGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATTGGGCTGGGTTGTGGAGAGGGTGGAGAGGTC
GTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGAACCGACAGTTTCTCTTCCGGCCCTGGGATG
TCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCGCCAGATGAATCCTTACATCCAGGTGACAAG
CCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTATGATGATGATTTCTTCCAAAATTTGGATGGT
GTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACATGGATCGCCGATGTGTGTACTATCGGAAGC
CACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGTGCAGGTGGTAATCCCCTTCCTGACAGAATC
CTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCCATTTGTACCCTGAAAAACTTTCCCAATGCC
ATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAGGCCTTTTCAAGCAGCCAGCAGAAAATGTTA
ATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATTGCGGCTGGCTGGTACCCAGCCATTGGAGGT
GCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCACAGACTTGGGGAGACTGTGTGACCTGGGCC
TGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGCAACTGCTGCACAACTTTCCTCCTGACCAGC
TCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACGCTGTCCACACCCACTTACTTTTGATGTTAA
CAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAACCTTTTTGCCCAGACCTATGGGTTGACTGGA
TCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAGTACAAGTCCCAGAGTTCACCCCCAAGTCTG
GTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGCCAATGCCTCTGTTGATGACAGCCGTCTTGA
GGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCCGGATTTAAGATGTACCCCATTGATTTTGAG
AAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGGCTGCATCCAATCTTCGGGCCGAAAACTATG
ATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACAAC
CACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTACAAGGTAGTTCAAGGGCACCAACAGCTCGAT
TCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCTTTGGGTTTTCTGAACCTCTTGCTGCACCTC
GTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCGCTTTGAAGTACAAGGGCTGCAGCCTAATGG
TGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAGACAGAGCACAAATTGGAGATCACCATGCTG
TCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAGCTGCTAAGCTCAAGGAACGATTGGATCAGC
CGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCTGGGCCGCCATGTGCGGGCACTGGTGCTTGA
GCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTCCCTTATGTCCGATATACCATTCGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009457
ORF Size 3357 bp
Insert Size 3357
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq NM_009457.4, NP_033483.2
RefSeq Size 4068
RefSeq ORF 3357
Locus ID 22201
Gene Summary This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Transcript Variant: This variant (1) uses an alternate 5' exon structure and an upstream start codon, compared to variant 3. The encoded isoform (1) has a longer N-terminus, compared to isoform 2.

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