Gcnt2 (NM_008105) Mouse Tagged ORF Clone

CAT#: MR219343

Gcnt2 (Myc-DDK-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gcnt2
• Synonyms: 5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219343 representing NM_008105
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCGTCCGTGCGTTACTTCTTCATAGTGTCTGTGACTACTGTCATCGTTTTTATTGTGCTCTACG
  TGTTAAGTTTTGGAGGAGATCAAAGCTACCAGAAGCTGAACATCTCAGACTCGGTGATGCTGGCTCAAGT
  GTGCTCATCTTTCATCGACGGGAAAAGCCGGTTCCTGTGGAGAAACAAACTAATGATTCACGAGAAGCCT
  TCTTGCACAGAATATGTGACCCAAAGCCACTATATCACTGCCCCTTTATCTCAGGAAGAGGTCGATTTTC
  CTTTGGCGTATGTCATGGTCATCCATCACAATTTTGACACCTTTGCAAGGCTCTTCAGGGCAATCTTCAT
  GCCTCAAAATATCTACTGTGTTCACGTGGATGAAAAGGCAACAGCTGAATTCAAAGGTGCCGTGGAACAG
  TTAGTGAGCTGCTTCCCCAATGCCTTTCTGGCTTCTAAGATGGAGCCGGTGGTCTATGGTGGAATCTCCC
  GGCTCCAGGCTGACCTGAACTGCATCAAAGACCTGTCCACCTCCGAGGTCCCCTGGAAGTACGCCATCAA
  CACCTGTGGACAAGACTTCCCCCTGAAAACCAACAAGGAGATAGTTCAGTACCTGAAAGGGCTTAAGGGG
  AAGAACCTCACTCCCGGGGTGCTGCCTCCAGCCCACGCCATTGGAAGGACCAGGTACGTCCACCGGGAAC
  ACCTAAGCAAAGAGCTTTCCTACGTGATCAGAACCACGGCTCTGAAGCCCCCACCTCCCCACAACCTCAC
  CATTTATTTTGGCTCTGCCTATGTCGCCCTGTCGAGAGAGTTTGCTAACTTTGTTCTCCGTGACCCACGG
  GCGGTTGATTTGCTCCATTGGTCCAAAGATACTTTCAGTCCCGATGAGCATTTCTGGGTGACGCTCAATA
  GGATTCCAGGAGTCCCTGGCTCCATGCCACCAAACGCATCCTGGACGGGTAACCTCAGAGCTGTGAAGTG
  GATGGACATGGAAGCGAAGCATGGAGGCTGCCATGGTCACTACGTCCATGGCATTTGTATCTATGGAAAC
  GGAGACTTGCAGTGGCTGATTAATTCGCAAAGCCTGTTTGCTAACAAATTTGAACTCAACACATACCCTC
  TTACCGTGGAATGCCTGGAACTGAGGCTTCGAGAAAGAACACTCAACCAGAGTGAGATCGCCATACAGCC
  GAGCTGGTATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219343 representing NM_008105
  Red=Cloning site Green=Tags(s)
  
  MPPSVRYFFIVSVTTVIVFIVLYVLSFGGDQSYQKLNISDSVMLAQVCSSFIDGKSRFLWRNKLMIHEKP
  SCTEYVTQSHYITAPLSQEEVDFPLAYVMVIHHNFDTFARLFRAIFMPQNIYCVHVDEKATAEFKGAVEQ
  LVSCFPNAFLASKMEPVVYGGISRLQADLNCIKDLSTSEVPWKYAINTCGQDFPLKTNKEIVQYLKGLKG
  KNLTPGVLPPAHAIGRTRYVHREHLSKELSYVIRTTALKPPPPHNLTIYFGSAYVALSREFANFVLRDPR
  AVDLLHWSKDTFSPDEHFWVTLNRIPGVPGSMPPNASWTGNLRAVKWMDMEAKHGGCHGHYVHGICIYGN
  GDLQWLINSQSLFANKFELNTYPLTVECLELRLRERTLNQSEIAIQPSWYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008105
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008105.2, NM_008105.3, NP_032131.2
• RefSeq Size: 3973
• RefSeq ORF: 1206
• Locus ID: 14538
• MW: 46.1 kDa
• Gene Summary: Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells. [UniProtKB/Swiss-Prot Function]